DS 11.121 DYE STAINS OF GENERAL APPLICATION 285 



satisfactory results. The phosphomolybdic and phosphotungstic hematoxylins of Mallory, 

 though originally intended for staining nervous structures, are useful for a more general 

 purpose. Mayer's 1891 "haemacalcium" was originally intended for staining wholemounts 

 of small marine invertebrates and is admirable for the purpose. 



11.121 Formulas Incorporating Iron Mordants 



11.121 Anderson 1929 Anderson 1929, 129 



STOCK solutions: I. 0.5% hematoxylin in 50% ale. 100, 2% calcium hypochlorite 5; II. 



water 100, ferric alum 3, sulfuric acid 2.5 

 WORKING solution: stock solution I 60, stock solution II 30 



note: Overstaining of sections rarely occurs but may be corrected with 0.1% hydro- 

 chloric acid in 70% alcohol. 



11.121 Barrett 1932 see DS 22.11 Barrett 1932 



11.121 Faure 1924 6639, 90:87 



reagents required: .4. 90% alcohol 100, hematoxylin 3.2; B. ADS 12.1 Faure 1924; 



C. 1% hydrochloric acid; D, sat. aq. sol. lithium carbonate 

 method: [distilled water] —> A + B, (equal parts), 5 sees. —> wash, tap water—* C, 



quick rinse -^ wash, tap water — » D, till blue — > wash -^ [counterstain, if desired] — > 



balsam, via usual reagents 



11.121 Hansen 1905 23632, 22:55 



formula: water 100, ferric alum 4.5, hematoxylin 0.75 



preparation: Dissolve the alum in 65 water. Dissolve the dye in 35 water. Mix. Boil. 

 Cool. Filter. 



11.121 Held test. 1937 Gatenby and Painter Gatenby and Painter 1937, 374 



reagents required: A. 5% ferric alum; B. water 95, DS 11.124 Held 1937 5 

 method: [water] — > A, 24 hrs. -^ B, 12-24 hrs. -* A, till differentiated — ♦ balsam, via 

 usual reagents 



11.121 Janssen 1897 6011,14:207 



formula: water 70, ferric alum 5, hematoxylin 1, abs. ale. 5, glycerol 15, methanol 15 

 preparation: Dissolve the alum in the water and the hematoxylin in the ale. Mix. 



Leave 1 week. Filter and add remaining ingredients. 

 note: This formula is recommended by Lillie and Earle 1939 (20540b, 14:53) as a sub- 

 stitute for Weigert 1904. 



11.121 Kefalas 1926 11360, 46:277 



formula: acetone 100, ferric chloride 1, hydrochloric acid 0.05, hematoxylin 1 



11.121 Krajian 1950 Krajian 1950, 196 



formula: water 50, 95% ale. 50, hematoxylin, ferric alum, ferric chloride, potassium 



iodide a.a. 6 

 preparation: Dissolve salts in water and dye in ale. Mix. 

 note: This formula, designed for bacteriology (see DS 23.222 Krajian 1950), is an 



excellent general-purpose hematoxylin. 



11.121 La Manna 1937 23632, 54:257 



formula: water 100, hematoxylin 1, ferric chloride 3 



11.121 LilUe and Earle 1939 608b, 15:765 



STOCK solutions: I. 95% ale. 50, glycerol 50, hematoxylin 1; II. water 100, ferric alum 



15, ferrous sulfate 15 

 working stain: stock I, 50, stock II 50 



11.121 Lillie 1940 1789a, 29:705 



formula: water 100, ferric chloride 1.2, hematoxylin 1, hydrochloric acid 1 



