286 METHODS AND FORMULAS DS 11.121-DS 11.122 



11.121 Morel and Bassal 1909 11024, 45:632 



STOCK solutions: I. 95% ale. 100, hematoxylin 1; II. water 100, ferric chloride 2, copper 



acetate 0.04, hydrochloric acid, 1 

 woKKiNG solution: stock I 50, stock II 50 



11.121 Olivecrona 1917 see DS 21.21 Olivecrona 1917 



11.121 Paquin and Goddard 1947 4349, 27:198 



formula: water 75, 95% ale. 25, glycerol 13, ferric alum 5, ammonium sulfate 0.7, 



hematoxylin 0.8 

 preparation: Dissolve the dye in the glycerol and ale. with gentle heat. Cool and add, 



with constant agitation, the other ingredients dissolved in the water. Leave 24 hours. 



11.121 Rozas 1935 23632, 52:1 



reagents required: A. water 74, 95% ale. 6, glycerol 20, ferric alum 1, aluminum 



chloride 1.2, hematoxylin 0.6; B. 20% ferric alum 

 method: [sections] -> water -^ yl, 12-24 hrs. — > fi, till differentiated —> balsam, via 



usual reagents 



11.121 Seidelin 1911 see DS 11.121 Weigert 1904 (note) 



11.121 Thomas 1943 4285a, 20:212 



formula: water 60, dioxane 40, acetic acid 6, hematoxylin 2.5, ferrous chloride 6, 



ferric chloride 1.5, ferric alum 3, hj'drogen peroxide 1 

 preparation: Dissolve the hematoxylin in the dioxane and add the hydrogen peroxide. 



Dissolve the salts in the water and acid. Filter into the hematoxylin solution. 



11.121 Verhoeff 1908 see DS 21.13 Verhoeff 1908 



11.121 Weigert 1903 test. 1910 ips. Ehrlieh, Krause, et al. 1910, 1:231 



stock solutions: I. 0.4% ferric chloride; II. 1% hematoxylin in 95% alcohol 

 reagents required: A. stock I 50, stock II 50; B. ADS 21.1 Masson 1942 

 method: [distilled water] -^ A, 1-2 hrs. —>■ distilled water, rinse —^ B, till differentiated 

 —^ tap water, till blue — » balsam, via usual reagents 



11.121 Weigert 1904 23632,21:1 



stock solutions: I. water 95, ferric chloride 0.6, hydrochloric acid 0.75; II. 1% hema- 

 toxylin in 95% ale. 



REAGENTS REQUIRED: A. StOCk I 50, StOck II 50 



METHOD : [distilled water] — » stain, till sufficiently colored — > distilled water, wash — > 

 tap water, till blue -^ balsam, via usual reagents 



note: See Chapter 21, DS 21.212 for use of these reagents in nerve staining. Weigert 

 diluted his B stock from a 10% stock just before use and specified a German pharma- 

 ceutical preparation, here reduced to terms of laboratory reagents, for his solution A. 

 Seidelin 1911 (16035, 4:94) used 3 stock A to 2 stock B in preparing solution .4. 



11.121 Yasvoyn test. 1946 Roskin Roskin 1946, 150 

 STOCK solutions: I. 0.1% hematoxylin; II. 2.5% ferric alum 



WORKING solutions: add II with constant stirring to 20 drops I until solution just 



remains blue 

 method: [sections] —> stain, 2-5 mins. —♦70% ale. if differentiation required — * balsam, 



via usual reagents 



11.122 Formulas Incorporating Alum Mordants 



11.122 Belloni 1939 lest. 1943 Cowdry Cowdry 1943, 35 



formula: water 100, potassium alum 3, hematoxylin 0.15, chloral hydrate 0.1, potas- 

 sium hydroxide 0.01 



11.122 Bohmer 1868 1780,4:345 



STOCK solutions: I. 3.5% hematoxylin in abs. ale.; II. 0.3% potassium alum 

 WORKING formula: stock I 10, stock II 90 



