DS 11.122 DYE STAINS OF GENERAL APPLICATION 287 



11.122 Bullard, in verb. Harpst 1951 



formitla: water 225, 95% ale. 'Mi, glycerol 'S'3, acetic acid 3.5, anunonium aliun 6, 

 mercuric oxide 0.8, hematoxylin 0.8 



I'KiorARATioN: Dissolve the dye in 15 of 50% ale. with 2 acetic acid. Heat and add 2 am- 

 nioniinu alum in 25 water. Boil, add mercuric oxide, and filter. Add remaining in- 

 gredients to cold filtrate. 



note; The author has not been able to discover any literature reference for this solution 

 which is widely used in pathological laboratories. 



11.122 Cajal and de Castro 1933 Cajal and de Castro 1933, 77 



PREPARATION OF STOCK: To 100 of a 5% solution of hematoxylin add 5 ammonia. 



Evaporate to dryness. 

 STAINING solution: 2% solution of above powder in 50% ale. 50; 5% ammonium alum 



50 



11.122 Carazzi 1911 23632, 28:273 



REAGENTS REQUIRED: A. water 80, potassium alum 5, hematoxylin 0.1, potassium iodate 

 0.02, glycerol 20; B. 0.1% hydrochloric acid in 70% 



METHOD FOR WHOLE OBJECTS: water -^^ lA + 10 water, 3 to 10 mins. — > distilled water 

 -+ B, if differentiation necessary — > tap water till blue -^ balsam, via usual reagents 



METHOD FOB SECTIONS: water -^ A, till sufficiently stained, 5 mins. to 12 hrs. -^ dis- 

 tilled water, wash — * tap water, till blue — > balsam, via usual reagents 



NOTE : A detailed description of the use of this stain in the preparation of a wholemount 

 is given under DS 11.10 above. 



11.122 Delafield test. 1885 Prudden 23632, 2 :288 



REAGENTS REQUIRED: A. Water 70, ammonium alum 3, hematoxylin 0.6, abs. ale. 4, 



glycerol 15, methanol 15 

 method: as Carazzi 1911 

 note: The original calls for preparation from sat. aq. sol. ammonia alum and 16% 



hematoxylin solution. 



11.122 Friedlander 1882 Friedlander 1882, 92 



formula: water 30, glycerol 30, 95% ale. 30, hematoxylin 0.6, potassium alum 0.6 



11.122 Gage 1892 test. 1896 ips. Gage 1896, 178 



formula: water 100, potassium alum 4, chloral hydrate 2, 95% ale. 2, hematoxylin 0.1 



11.122 de Groot 1912 23632,29:182 



FORMULA OF SOLVENT: 95% alc. 65, water 27, glycerol 8 



formula of stain: 95% alc. 65, water 27, glycerol 8, hydrogen peroxide 0.75, hema- 

 toxylin 0.2, calcium chloride 1.5, sodium bromide 0.75, ammonium alum 2.2, potassium 

 ferricyanide 0.08 



PREPARATION OF STAIN: Take 100 solvent. Mix 1.5 solvent with 0.75 hydrogen peroxide 

 and dissolve 0.2 hematoxylin in this. In 25 solvent dissolve 15 calcium chloride and 

 0.75 sodium bromide. Add this to the hematoxylin solution and dissolve 1.1 am- 

 monium alum in the mixture. In 40 solvent dissolve 0.08 potassium ferricyanide and 

 add this to mixture. In the remaining solvent dissolve 1.1 ammonium alum and add 

 to the mixture. 



11.122 Harris 1900 11032,3:777 



formula: 95% alc. 5, hematoxylin 0.5, potassium alum 10, water 100, mercuric oxide 



0.25 

 preparation : Dissolve the hematoxylin in alc. Dissolve alum in water and raise to boil- 

 ing. Pour hot solution on hematoxylin. Boil and throw mercuric oxide into boiling 

 solution. Cool rapidly. Filter. 

 note: Mallory 1938, 72 suggests the addition of 5% acetic acid. 



11.122 Harris and Power test. 1884 Cole Cole 1884b, 42 



formula: hematoxylin 20, alum 60, water 100, abs. alc. 6 



preparation: Grind the hematoxylin in a mortar with the alum, adding water in small 

 portions while grinding. Filter and add alcohol. 



