DS 11.122-DS 11.123 DYE STAINS OF GENERAL APPLICATION 289 



11.122 Sass 1929 20540b, 4:127 



formula: water 100, ammonium alum 5, hematoxylin 0.1, sodium iodate 0.1 

 note: See also DS 11.123 Sass 1929. 



11.122 Tribondeau, Fichet, and Dubreuil 1916 6630, 79:288 



FORMULA FOR STOCK SOLUTION: 95 alc. 100, Water 22, silver nitrate 2, sodium hydroxide 

 1, hematoxylin 5 



PREPARATION OF STOCK SOLUTION: Add the hydroxide dissolved in 20 water to the silver 

 nitrate dissolved in 2 water. Wash ppt. by decantation and transfer to flash with re- 

 flux condenser. Dissolve the hematoxylin in alc. and add to silver suspension. Raise 

 to boiling, cool, filter. 



WORKING solution: 5% potassium alum 100, stock 5 



11.12S Forrmilas Incorporating Acid Alum Mordants 



11.123 Anderson 1923 test. 1929 i-ps. Anderson 1929, 192 



REAGENTS REQUIRED: A. watcr 90, abs. alc. 5, hematoxylin 0.25, calcium hypochlorite 

 0.4, ammonium alum 2, acetic acid 5; B. 0.1% hydrochloric acid 



PREPARATION OF A : Add the hypochlorite to 20 water. Leave 4 hours. Filter. Add filtrate 

 to hematoxylin dissolved in water. Dissolve other ingredients in rest of water. Add 

 this to dye solution. 



method: water -> A, 2-3 mins. — > B, till differentiated -^ tap water, till blue 



11.123 Anderson 1929 Anderson 1929, 129 



formula: water 70, 95% alc. 5, acetic acid 5, calcium hypochlorite 4, ammonium alum 3, 



hematoxylin 0.5 

 preparation: as Anderson 1923. 



11.123 Apathy 1897 14246, 12:712 



formula: water 45, 95% alc. 25, glycerol 35, hematoxylin 0.3, acetic acid 1, salicylic 



acid 0.03, ammonium alum 3 

 preparation: Dissolve the hematoxylin in 10 water, 25 alc. Allow to ripen for some 



months. Dissolve the alum and acids in 35 water. Add to dye solution; then add 



glycerol. 



11.123 Cole 1903 Cross and Cole 1903, 170 



formula: water 32, 95% alc. 32, glycerol 29, acetic acid 7.5, hematoxylin 0.6, am- 

 monium alum 0.6 

 preparation: Add alum dissolved in water to hematoxylin dissolved in alc. Add other 

 ingredients to mixture. 



11.123 Conklin test. 1930 Guyer Guyer 1930, 232 



formula: water 80, DS 11.122 Delafield 1885 20, F 5000.0050 Kleinenberg 1879 4 



11.123 EhrUch 1886 23632, 3:150 



reagents required: A. water 30, 95% alc. 30, glycerol 30, acetic acid 3, hematoxylin 

 0.7, ammonium alum to excess; B. sat. 70% alc. sol. lithium carbonate. ^ 



preparation of a : Dissolve hematoxylin in the alc. and acid. Dissolve 1 ammonium 

 alum in water and glycerol. Mix with dye solution. Allow to ripen for some months. 

 Add excess {circ. 10) ammonium alum to ripened solution. 



method for sections: 90% alc. — ► A, Y^ to 2 mins. -+ 90% alc. applied from drop 

 bottle, till differentiated — > B, till blue -+ balsam, via usual reagents 



note: The passage of sections to water before staining, or the use of water to differ- 

 entiate, results in a diffuse stain. A detailed description of the use of this stain for 

 sections is given under DS 11.10 above. 



11.123 Harris 1900 see DS 11.122 Harris 1900 (note) 



11.123 Langeron 1942 Langeron 1942, 523 



reagents required: .1. DS 11.122 Mayer 1901 100, chloral hydrate 5, citric acid 0.1; 



B. 0.1% HClin70% alc. 

 method: [sections or whole objects]-^ distilled waters A, (sections) 10 mins. or A, 

 (whole objects) 24 to 48 hrs. -^ B, till differentiated -> tap water tiU bl.ue -» balsam, 

 yig, usual reagents 



