290 METHODS AND FORMULAS DS 11.12a-DS 11.124 



11.123 LiUie 1941 20540b, 16 :5 



formula: water 70, glycerol 30, acetic acid 20, hematoxylin 0.5, sodium iodate 0.1 

 preparation: Add the iodate to the dye dissolved in the water. Leave overnight. Add 



other ingredients. 

 note: This formula was republished, without any reference to its previous publication, 



by Lillie 1942 (20540b, 17:90). 



11.123 Mallory 1938 see DS 11.122 Harris 1900 (note) 



11.123 Mann 1892 ksl. 1934 Langeron Langeron 1934, 475 



formula: water 35, 95% ale. 32, glycerol 25, acetic acid 3, hematin 0.6, potassium 



alum 3.5 

 preparation: Dissolve the dye in the acid. Add mixed ale. and glycerol. Then add alum 



dissolved in water. 



11.123 Masson test. 1934 Langeron Langeron 1934, 475 



formula: water 100, acetic acid 2, hematin 2, potassium alum 6 

 preparation: Dissolve the alum in boiling water. Add dye, cool, filter. Add acid to 



filtrate. 

 note: a detailed description of the use of this stain before a complex contrast is given 

 under DS 12.30 below. 



11.123 Mayer 1891 Maj/er's acid hemalum — auct. see DS 11.122 Mayer 1891 (note) 



11.123 Mayer test. 1924 Langeron Langeron 1942, 525 



note: This is DS 11.122 Mayer 1896 with the addition of acetic acid. Mayer, however, 

 recommended this addition only to his 1891 formula. The present solution is, there- 

 fore, Langeron's variant. 



11.123 Sass 1929 20540b, 4:127 



formula: water 100, ammonium alum to sat., hematoxylin 1, sodium iodate 1, acetic 

 acid 3 



11.123 Watson 1943 11360, 63:20 



formula: water 32, abs. ale. 32, glycerol 32, acetic acid 3, ammonium alum 0.064, 



hematoxylin 0.64, potassium permanganate 0.032 

 preparation: Dissolve the alum and permanganate in water. Add dye dissolved in ale. 



and then other ingredients. 

 note: This formula stains as well as DS 11.123 Ehrlich 1886, from which Watson de- 

 veloped it, and does not require ripening. 



11.134 Formulas Incorporating Other Mordants 



11.124 Alzheimer 1910 lithium-heinatoxylin Nissl and Alzheimer 1910, 411 

 formula: water 90, 95% ale. 10, hematoxylin 1, Hthium carbonate 0.03 

 preparation: Add the alkali dissolved in water to the dye dissolved in ale. 



11.124 Bacsich 1937 lithium-hematoxylin 11025,72:163 



formula: water 100, hematoxylin 1, lithium carbonate 0.1 



11.124 Clara 1933 molybdic-hematoxylin 23632,60:73 



stock solution: 1% hematoxylin 50, 10% ammonium molybdate 50, molybdic acid to 



excess (circ. 1) , 



working solution: stock 0.1, water 100 



11.124 Cook test. 1883 Hogg copper-hematoxylin Hogg 1883, 237 



formula: water 100, "extract of logwood" 15, copper sulfate 2.5, potassium alum 15 

 preparation: Grind the dry powders in a mortar. Add enough water to make a paste. 



Leave two days; add rest of water. Leave 12 hours. Filter. 

 note: The "extract of logwood" is the result of evaporating an aqueous extract of log- 

 wood to dryness; in addition to hematoxylin and hematin (about 70% of the whole) 

 it contains tannin, glucosides, and resins. 



