DS 11.124 DYE STAINS OF GENERAL APPLICATION 291 



11.124 Cretin 1925 ferricyanide-hematoxylin LeMans, 93 



REAGENTS REQUIRED: A. Water 100, ferrous sulfate 4; B. water 100, potassium fcrro- 



cyanide 2, potassium fcrricj'anide 1; C. water 100, hematoxylin 0.5; D. water 100, 



ferric alum 5 

 method: Distilled water — > A, 24 hrs. -^ running tap water, overnight —^ B, 3-6 hrs. — » 



distilled water, rinse -^ C, overnight -^ D, till differentiated 

 results: nuclei dense, opaque black 



11.124 Donaggio 1904 lin-hernatoxylin 1006,22:192 



formula: water 100, hematoxylin 0.5, stannic chloride diammine 10 

 preparation: Dissolve the dry salts each in 50 water. Mix solutions. 

 note: Donaggio 1940 (18208, 22:171) substitutes stannic chloride for the diaminine 

 complex. 



11.124 Gomori 1941 chrome-hemaloxylin 608b, 17, 395 



formula: water 100, sulfuric acid 0.1, potassium dichromate 0.1, chrome alum 1.5, 



hematoxylin 0.5 

 preparation: Dissolve the alum and dye each in 50. Mix. Add 2 5% potassium di- 

 chromate and 2 5% sulfuric acid. Ripen 2 days. 



11.124 Hansen 1905 chrome-hemaioxylin 23G32, 22 :64 



formula: water 100, sulfuric acid 0.2, hematoxylin 0.3, chrome alum 3, potassium 



dichromate 0.2 

 preparation: Boil the chrome alum in 85 water until green. Dissolve hematoxylin in 



5 water. Add to alum solution. Then add successively, acid in 2 water and dichromate 



in 7 water with constant stirring. Filter. 



11.124 Harris 1900 mercuric-hematoxylin see DS 11.122 Harris 



11.124 Heidenhain test. 1907 Bohm and Oppel vanadium-hematoxTjlin 



Bohm and Oppel 1907, 105 

 formula: 0.5% hematoxylin GO, 0.25% ammonium vanadate 30 



11.124 Held test. 1937 Gatenby and Painter phosphottwlybdic hematoxylin 



Gatenby and Painter 1937, 374 

 formula: water 30, 95% ale. 70 hematoxylin 1, phosphomolybdic acid 15 

 preparation: Dissolve hematoxylin in solvents. Add acid. Leave 1 month. Decant. 



11.124 Hornyold 1915 test. Gatenby and Painter 1937 iodine-he matoxylin 



Gatenby and Painter 1937, 158 

 reagents required: A. a])s. ale. 25, water 75, hematoxyhn 0.8, ammonium alum 0.5, 



tincture of iodine USP (see note) 0.5; B. 0.1% acetic acid in 70% ale. 

 preparation of a : Add the alum dissolved in the water to the hematoxylin dissolved in 



the ale. Ripen some days. Add iodine. 

 method: [sections] —> water -^ /I, 5-10 mins. -^ rinse -^ B, till blue —> balsam, via 



usual reagents 

 note: The tincture of iodine mentioned in the original formula is the British, which 



contains 2li % each of I; and KI in 90% ale. The official American tincture is double 



this concentration and should be used in the preparation above. 



11.124 Hueter 1911 see DS 11.124 Schueninoff 1908 (note) and Mallory 1891 (note) 



11.124 Kleinenberg 1876 and 1879 see DS 11.124 Squire 1892 (note) 



11.124 Liengme 1930 iron-copper-hematoxylin 4285a, 7:233 



REAGENTS REQUIRED: A. DS 11.122 Bohmcr 1868 50, ADS 12.1 Morel & Bassal 1909 50; 



B. 0.1% hydrochloric acid; C. sat. sol. lithium carbonate in 70% ale. 



method: 70% alcohol -^^ A, 1-4 days ^ B, till differentiated -» 70% alcohol, wash -> 



C, till blued — » balsam, via usual reagents 



11.124 Loyez test. 1938 Carleton and Leach lithium hematoxylin 



Carleton and Leach 1938, 259 

 STOCK i: abs. ale. 100, hematoxylin 10; stock ii: water 100, sat. aq. sol. lithium carbon- 

 ate 4 

 WORKING solution: stock I 10, stock II 90 



