292 METHODS AND FORMULAS DS 11.124 



11.124 Mallory 1891 phosphomolybdic hematoxylin 766,7:375 



formula: water 100, hematoxylin 1, phosphomolybdic acid 1, chloral hydrate 7.5 

 preparation: Add the acid dissolved in 10 water to the dye dissolved in 90. Add chloral 



hydrate to mixture. 

 method: water —> stain, 10 mins. to 1 hr. — > 30% ale. till differentiated 

 note: Phenol may be substituted for chloral hydrate. Hueter {test. Schmorl 1928, 173) 



substitutes phosphotungstic for phosphomolybdic acid; but see note under Schuenin- 



off 1908. 



11.124 Mallory 1900 phosphotungstic hematoxylin 11189,5:19 



REAGENTS REQUIRED: A. water 100, hematoxylin 0.1, phosphotungstic acid 2, hydrogen 



peroxide 0.2 

 preparation: Add the acid dissolved in 20 water to the dye dissolved in 80. Add 



hydrogen peroxide to mixture. 

 method: [sections of material fixed in F 3700.0010 Zenker 1894 or similar fixative] -* 



water, thorough wash -^ A, 12-24 hrs. -* 95% alcohol, about one minute-^ abs. ale. 



till differentiation complete — > balsam, via xylene 

 note: This is a polychrome, general-purpose stain. 



11.124 Mallory test. McClung 1929 phosphotungstic hematoxylin 



McClung 1929, 288 

 reagents required: A. 0.25% potassium permanganate; B. 5% oxalic acid; C. water 



100, hematoxylin 0.1, phosphotungstic acid 2, potassium permanganate 0.025 

 method: [sections of material fixed in F 3700.0010 Zenker 1894 or similar fixative]-^ 

 A, 5-10 mins. -> water, thorough rinse -^ B, 10-20 mins. -^ C, 12-24 hrs. -^ 95% 

 ale, quick rinse -^ abs. ale, least possible time -^ balsam via xylene 

 note: This is a polychrome general-purpose stain, 



11.124 Mayer 1891 Haemacalcium—auct. 14246,10:182 



REAGENTS REQUIRED: A. water 30, ale. 70, acetic acid 1.5, hematoxylin 0.15, aluminum 



chloride 0.15, calcium chloride 7.5; B. 95% ale. 30, water 70, aluminum chloride 2 

 PREPARATION OF A : Grind the dye with the aluminum chloride in a beaker. Add solvents 



and warm to solution. Then add calcium chloride. 

 method: [whole objects] ^ 70% ale. -^ ^, till stained, usually overnight -+ B, till 



differentiated — > balsam, via usual reagents 

 note: Mayer 1910 " Haemastrontium" {test. Gatenby and Cowdry 1937, 160) differs 



from above only in substitution of strontium chloride for calcium chloride, and of 



0.1 citric acid for 1.5 acetic acid. 



11.124 Mayer 1910 Haemastrontium — auct. see DS 11.124 Mayer 1891 (note) 



11.124 Police 1909 phosphomolyhdic-hematoxylin 1949, 4:300 



formula: water 70, ale. 30, chloral hydrate 10, hematoxylin 0.35, phosphomolybdic acid 

 0.03 



11.124 Rawitz 1909 aluminum-hematoxylin 23632,25:391 



formula: water 50, glycerol 50, hematin 0.2, aluminum nitrate 2 



preparation: Add the aluminum nitrate dissolved in 25 water to the dye dissolved in 25 

 water. Then add glycerol. 



11.124 Schroder 1930 lilhium-hematoxylin 23430, 166, 588 



formula: water 100, hematoxylin 0.3, lithium carbonate 0.04 



11.124 Schueninofi 1908 phosphomolybdic hematoxylin 23681,18:6 



formula: water 100, hematoxylin 0.9, phenol 2.5, phosphomolybdic acid 0.5 

 note: Hueter 1911 (Romeis 1948, 351) differs from this only in the substitution of 

 phosphotungstic acid; but see note under Mallory 1891. 



11.124 Schweitzer 1942 /(.■?/. 1946 Roskin chrome-hematoxylin 



Roskin 1946, 200 

 formula: water 125, chrome alum 5, hematoxylin 0.5, 10% sulfuric acid 4, potassium 



dichromate 0.275 

 preparation: To the first three ingredients dissolved iu 90 water add the dichromate 

 dissolved in 10. 



