DS 11.2^DS 11.24 DYE STAINS OF GENERAL APPLICATION 303 



11.23 Zirkle 1940 see M 11.1, M 12.1, M.13.1, M 22.1 and M.31.1 Zirkle 1940 



11.24 PICRO-CARMINES 



11.24 Arcangeli test. circ. 1890 Francotte Francotte, 217 

 formula: water 100, picric acid 1, carmine 0.5 

 prepar.\tion: Boil 10 minutes. Cool. Filter. 



11.24 Bizzozero test. 1889 Friedlander Friedlander 1889, 89 



formula: water 100, 95% ale. 20, picric acid 1, carmine 1, ammonia 6 

 preparation: Dissolve carmine in ammonia. Add 100 water. Dissolve picric in 100 

 water. Add to carmine and evaporate to 100. Cool; filter. Add ale. to filtrate. 



11.24 Francotte circ. 1890 Francotte, 216 



formula: water 100, carmine 1, ammonia 5, picric acid 1, chloral hydrate 1 

 preparation: Dissolve carmine in ammonia. Dissolve picric acid in 50 hot water and 

 add to carmine. Dilute to 100. Add chloral hydrate. 



11.24 Friedlander 1889 Friedlander 1889, 88 



formula: water 50, carmine 1, ammonia 1, sat. sol. picric acid 100 

 preparation: Dissolve carmine in ammonia. Add water and picric solution. 



11.24 Gage 1880 (est. circ. 1890 Francotte Francotte, 213 



formula: water 100, carmine 1, ammonia 50, picric acid 1 



prepakation: Dissolve carmine in ammonia. Add picric dissolved in water. Leave 1 day. 

 Filter. Evaporate to dryness. Dissolve residue in 100 water. 



11.24 Guyer 1906 test. 1930 ips. Guyer 1930, 239 



formula: water 50, sat. aq. sol. picric acid 50, ammonia 5, carmine 1 

 preparation: Dissolve carmine in ammonia. Add water and picric solution. Leave 2 

 days. Filter. 



11.24 Jensen 1937 23684, 139:333 



STOCK solutions: I. water 100, 0.1, carmine 0.5; II. water 100, picric acid 0.5, mag- 

 nesium oxide 2 

 WORKING solution: stock I 80, stock II 20 



11.24 Legal 1884 14555, 8:353 



bormula: DS 11.21 Grenacher 1897 90, sat. aq. sol picric acid 10 



11.24 Lowenthal 1892 see DS 13.7 Lowenthal 1892 



11.24 Lowenthal test. 1900 Pollack Pollack 1900, 79 



formula: water 100, sodium hydroxide 0.5, carmine 2, picric acid q.s. 

 preparation: Dissolve carmine and alkali in 50 boiling water. Boil 15 minutes. Dilute 

 to 100 and cool. Add picric acid in excess of saturation. 



11.24 Malassez test. 1877 Frey Frey 1877, 96 



formula for dry stock: water 100, ammonia 2, carmine 0.5, picric acid 2.5 

 preparation for dry stock: Dissolve carmine in ammonia and water. Add picric. 



Shake; allow to settle; and decant. Evaporate supernatant to dryness. 

 FORMULA FOR WORKING SOLUTION: water 100, dry stock 2 

 preparation OF WORKING SOLUTION: Mix. Leave 1 week. Filter. 



11.24 Mayer 1897 23632, 14:23 



formula: DS 11.28 Mayer 1897 10, 0.6% magnesium picrate 90 



11.24 Neuman test. 1928 Schmorl Schmorl 1928, 120 



reagents required: A. DS 11.21 Grenacher 1879 100, picric acid 1.25; B. 2% hydro- 

 chloric acid in glycerol; C. anhydrous glycerol 

 method: [sections] — > A, 5-10 mins. — * B, 10 mins. -^ C 



note: To prepare balsam mounts, substitute a sat. sol. picric acid in abs. ale. for C above 

 and clear in clove oil. 



11.24 Oppler test. 1928 Schmorl ^- Schmorl 1928, 341 



formula: carmine 0.5, ammonia 0.5, picric acid 0.005, water 100 



