304 METHODS AND FORMULAS DS 11.24-DS 11.25 



11.24 Orth test. 1904 Besson Besson 1904, 255 



fokmula: DS 11.28 Orth (1892) 65, sat. aq. sol. picric acid 35 

 note: This same formula is given by Squire 1892 but without acknowledgment to Orth. 



11.24 Ranvier 1875 Ranvier 1875, 100 



PKEPARATiON OF DRY STOCK: Dissolve 5 Carmine in 50 ammonia. Add 500 sat. sq. sol. 



picric acid. Evaporate to 100. Cool 24 hours. Decant supernatant liquid which is 



evaporated to dryness. 

 WORKING solution: water 100, dry stock 5 



PREPARATION ot WORKING SOLUTION: Boil 10 minutes. Cool. Filter. 

 method: [anything, living or dead] -^ stain, till done — > 70% ale. till color clouds cease 



— > balsam, via usual reagents 

 note: The original formula called for a prolonged period of putrefaction in the course 



of manufacture. This stain, now practically unknown, should be placed in the hands 



of every beginner. A live Cyclops may be dropped into the solution and removed, fixed, 



and stained 10 minutes later; so may any other object. A description of a preparation 



utilizing this stain is given under DS 12.20 below. 



11.24 Rutherford test. 1878 Marsh Marsh 1878, 79 



formula: sat. sol. picric acid 100, carmine 1, ammonia 2, water 



preparation : Dissolve carmine in ammonia and 5 water. Raise picric solution to boiling 

 and add carmine solution. Evaporate to dryness. Dissolve residue in 100. 



11.24 Squire 1892a Squire 1892, 34 



formula: anmionia 1.5, carmine 0.5, water 2.5, sat. aq. sol. picric acid 100 

 preparation: Dissolve dye in ammonia. Add picric solution. 



11.24 Squire 1892b Squire 1892, 35 



formula: water 100, sodium hydroxide 0.05, carmine 0.5, picric acid q.s. 

 preparation: Dissolve the carmine in the boiling alkali sol. Add just enough sat. aq. sol. 

 picric acid to redissolve the ppt. first formed. 



11.24 Squire 1892c Squire 1892, 35 



formula: DS 11.28 Orth (1892) 25, sat. aq. sol. picric acid 75 

 note: See also Orth (1904) note. 



11.24 Vignal test. 1907 Bohm and Oppel cit. Henneguy Bohm and Oppel 1907, 118 

 formula: water 100, picric acid 2, carmine 1, ammonium hydroxide 5 

 PREP.\RATiON of DRY STOCK: Mix all ingredients. Leave 2-3 months in closed bottle. 

 Evaporate at room temperatures to 80. Decant and evaporate supernatant to dryness. 

 WORKING solution: water 100, dry stock 1 



11.24 Weigert 1881 22575,84:275 



formula: sat. aq. sol. picric acid 100, carmine 1, ammonia 2, acetic acid q.s. 

 preparation: Dissolve carmine in ammonia. Add picric solution and leave 24 hours. 

 Then add just enough acetic acid to produce permanent ppt. Filter. 



11.25 IRON carmines 



11.25 de Groot 1903 23632,20:21 



formula: water 100, hydrochloric acid 0.05, potassium alum 2.5, ferric alum 0.05, car- 



minic acid 0.5 

 preparation: Dissolve ferric alum in 10 water. Add dye and dilute to 100. Heat to 60°C. 



and add potassium alum. Cool. Filter. Add acid to cold filtrate. 



11.25 Hansen 1905 23632, 22:85 



formula: water 100, sulfuric acid 1, ferric alum 3, cochineal 3 

 preparation: Boil all ingredients 10 minutes. Cool. Filter. 



11.25 Lee 1902 test. 1905 ips. Lee 1905, 170 



reagents required: A. ADS 12.1 Benda 1893; B. 0.5% carminic acid in 50% alcohol 

 method: [sections] — > water -^ A, some hrs. — » rinse, 50% ale. -♦ B, 1-2 hrs. — » wash, 

 50% ale. — > balsam, via usual reagents 



