DS 11.25 DS 11.27 DYE STAINS OF GENERAL APPLICATION 305 



11.25 Peter 1904 23632, 21:314 



REAGENTS required: A. watcr 100, cochineal G, liydrocliloric acid 0.3; li. 2.5% ferric 



alum 

 PREPARATION OF a: Boll cochiiieal in 150 water till reduced to 30. Dilute with hot water 



to 100. Cool. Filter. Add acid. 

 method: [whole objects to be sectioned, or sections] — > A, 48 hrs. at 37°C. — » rinse —<■ B, 



some hrs. -^ balsam, or paraffin, via usual reagents 

 result: Said by Lee {Lov 1905, 171) to stain yolk granules red on a gray background. 



11.25 Spuler 1901 test. 1910 ips. Ehrlich, Krause, et. al. 1910, 1:240 



PREPARATION OF STOCK SOLUTION: Boil 20 cochineal 1 hour in 100 water. Filter, saving 

 both residue and filtrate. Boil residue in 100 water 1 hour. Filter, lleject residue. Mix 

 filtrates and reduce to 100. Add 95% ale. till ppt. appears. Filter. Reduce filtrate to 

 100. Filter. 

 REAGENTS required: A. stock 35, 50% alcohol 65; B. 0.75% ferric alum 

 method: 70% alcohol -^ A, 48 hrs. at 37°C. -^ wash -* B, 24 hrs. 



11.25 Wellheim 1898 23632, 15:123 



REAGENTS REQUIRED: A. 0.01% ferric chloride in 50% ale; B. 0.5% carminic acid in 50% 



ale; C. 0.1% hydrochloric acid in 70% ale. 

 method: [sections] -^^ 50% ale. — + A, overnight -^ rinse, 50% ale. -+ B, 1-2 hrs. — > 



wash, 50% ale. -^ C, till differentiated — > balsam, via usual reagents 



11.25 Zacharias 1894 see DS 11.23 Zacharias 1894 



11.26 AMMONIA CARMINES 



11.26 Beale 1857 test. 1880 Beale Beale 1880, 125 

 formula: carmine 0.5, ammonia 1.5, water 44, 95% ale. 12, glycerol 44 

 preparation: Dissolve carmine in ammonia. Add other ingredients. 



11.26 Cole 1903 Cross and Cole 1903, 169 



formula: water 100, carmine 1, ammonium hydroxide 2, sodium borate 6 

 preparation: As Beale 1857. 



11.26 Frey 1877 Frey 1877, 94 



formula: water 40, glycerol 40, 95% ale. 20, carmine 4, ammonia q.s. to dissolve 

 preparation: As Beale 1857. 



11.26 Gerlach 1858 tent. 1892 Squire Stpiire 1892, 31 



formula: water 100, ammonia 1, carmine 1 



note: Squire (loc. at.) and Lee (Lee 1905, 172) recommended that the solution be 

 allowed to grow mold, evaporated to dryness, and then redissolved in distilled water, 



11.26 Hoyer test. 1900 Pollack Pollack 1900, 78 



preparation of DRY STAIN : Dissolvc 2 carmine in 4 ammonia and IG water. Boil till 



smell of ammonia not apparent. Cool. Add 80 95% ale. Filter. 

 working solution: water 100, powder from above 0.5 



11.26 Meriwether 1935 4349, 14 :64 



formula: water 75, ammonia 25, potassium chloride 6.25, potassium carbonate 1.9. 



carmine 2.5 

 preparation: Boil the salts and the dye in the water 2 minutes. Cool and add ammonia. 



11.26 Smith test. 1903 Cross and Cole Cross and Cole 1903, 171 



formula: water 50, glycerol 25, 95% ale. 25, carmine 1, ammonia 1.5, sodium borate 1 

 preparation: As Beale 1857. 



11.26 Squire 1892 Squire 1892, 31 

 formula: water 40, 95% ale. 20, glycerol 40, carmine 2.5, ammonia 5 

 preparation: As Beale 1857. 



11.27 hydrochloric carmines 



11.27 Hollande 1916 Chlorcarmin—compl. script. 6630, 79 :662 



reagents required: A. water 90, 95% ale. 10, carmine 7, hydrochloric acid 2.5 water 

 q.s. to bring filtrate to 90, 70% ale. 10; B. 3% ferric alum; C. 1% pyridine 



