DS 11.41 DYE STAINS OF GENERAL APPLICATION 313 



11.41 SOLUBLE METALLIC LAKES OF THE OXAZINES 



11.41 Anonymous 1936 Catalogue of Vector Mfg. Co., 



London, n.d. (received 1936) 

 FORMUL.\: water 100, ferric alum 2.5, coelestin blue B 0.5, glycerol 14, sulfuric acid 2 

 preparation: Boil the dye 5 minutes in alum solution. Cool. Filter. Add other ingredients. 

 method: [sections]-^ water—* stain, 5 mins. to 1 hr. -^ water, wash -^ [counterstain] 



— > balsam, via usual reagents 

 note: a description of the use of this stain, before a complex contrast, is given under 

 DS 12.20 below. This is probably derived from DS 11.41 Lendrum 1935 {q.v.). 



11.41 Becher 1921a , Becher 1921, 46 



formula: water 100, ferric alum 5, napthopurpurin 0.5 

 preparation: Boil 5 minutes. Cool. Filter. 

 method: [sections] — ♦ water—* stain, 2 hrs. -^ water, wash — > [counterstain] — > balsam, 



via usual reagents 

 result: nuclei black 



11.41 Becher 1921b Becher 1921, 72 



formula: water 100, chrome alum 5, gallocyanin 0.5 

 preparation: as Becher 1921a. 



method: as Becher 1921a save that 24 hrs. staining is recommended 

 result: nuclei deep blue 



note: Buzaglo 1934 (4285a, 11:40) diminishes the gallocyanin to 0.1 and adds 1% 

 formaldehyde to the filtrate. 



11.41 Becher 1921c Becher 1921, 40 



formula: water 100, aluminum chloride 5, napthazarin 0.5 

 preparation: As Becher 1921a. 



method: as Becher 1921a save that 24 hrs. staining is recommended 

 result: nuclei, deep blue violet 



notes: Becher {loc. cit.) recommends also napthopurpurin (dark red nuclei), purpurin 

 (scarlet nuclei), and galloflavin (yellow nuclei) in solutions of aluminum chloride. 



11.41 Buzaglo 1934 see DS 11.41 Becher 1921b (note) 



11.41 Cole 1947 20540b, 22:103 



formula: water 100, chrome alum 5, gallocyanin 1.5 

 preparation: Boil the dye 5 minutes in the alum solution. 



11.41 Einarson 1932 and 1935 see DS 22.3 Einarson 1932 and 1935 



11.41 Lendrum 1935 11431,40:415 



formula: water 84, glycerol 14, sulfuric acid 2, ferric alum 4.2, celestin blue 0.42 

 preparation: Boil the dye in the alum 5 minutes. Cool. Filter. Add glycerol and acid. 



11.41 Lendrum and McFarlane 1940 sec DS 11.41 Proescher and Arkush 1928 (note) 



11.41 Petersen 1926 23632, 43:355 



formula: water 100, aluminum sulfate 10, gallocyanin 0.05 

 preparation: Boil 10 minutes. Cool. Filter. Make up to 100. 



11.41 Proescher and Arkush 1928 20540b, 3:36 



formula: water 100, ferric alum 5, coelestin blue B 0.5 



preparation: As Becher 1921a. 



method: [sections]—* water —^ stain, till nuclei deep blue black, 3 mins. to 2 hrs. — » 

 water, wash — * [counterstain] — * balsam, via usual reagents 



note: Proescher and Arkush {loc. cit.) also recommended gallocyanin blue and gallo- 

 cyanin in ferric alum .sohition; but for the latter see DS 13.5 Becher 1921. These solu- 

 tions are le.ss stable than coelestin blue B solutions. For coelestin blue B as a poly- 

 chrome stain see DS 21.16 Becher 1921. Lendrum and McFarlane 1940 (11431, 50:381) 

 add 14 glycerol to this solution. 



