314 METHODS AND FORMULAS DS 11.42 



11.42 SAFEANIN 



11.42 Babes 1887 23632, 4:470 



formula: water 100, aniline 2, safranin 7 



preparation: Heat to 60°C. for 1 hour stirring frequently. Cool. Filter. 



method: [sections of osmic fixed material] — > water -^ stain, till nuclei bright red, 1 hr. 

 to 10 days —> water, wash —^ balsam, via usual reagents 



note: This is what is usually meant by Babes Safranin even though Babes had pre- 

 viously (1883; 1780, 21:356) recommended both alcoholic and aqueous solutions. A 

 slight modification of this is given by Langeron 1934, (100) as "Babes-Langeron- 

 Dubosq." This method with a light green counterstain is sometimes called Benda's 

 stain. See also DS 12.15 Land (1915). 



11.42 Benda see DS 11.42 Babes (note) 



11.42 Bohm and Oppel 1907a Bohm and Oppel 1907, 209 



formula: water 72, ammonium carbonate 0.25, 40% formaldehyde 8, sat. ale. sol. 



safranin 

 preparation: Add the dye solution mixed with the formaldehyde to the carbonate 



solution. 

 method: [sections] —» water -^ stain, 24 hrs. — >95% ale, quick rinse —> water —* 



[counterstain] — > balsam, via usual reagents 



11.42 Bohm and Oppel 1907b Bohm and Oppel 1907, 113 



formula: water 85, 40% formaldehyde 5, 95% ale. 10, phenosafranin 1 



11.42 Chamberlain 1916 Chamberlain 1915, 51 



formula: water 100, 95% ale. 50, water soluble safranin 0.5, alcohol soluble safranin 0.5 

 preparation: Dissolve each dye in the appropriate solvent and mix the solutions. 

 note: For a rationalization of this procedure see Conn 1946, 109. 



11.42 "Dubosq" see DS 11.42 Babes (note) 



11.42 Johansen 1940 Johansen 1940, 62 



reagents required: A. 95% ale. 25, water 25, methyl cellosolve 50, safranin 0.1, 



sodium acetate 1, 40% formaldehyde 2; B. ADS 22.1 Masson (1942) 

 preparation op A: Dissolve the dye in the cellosolve. Add first ale, then water. Then 



add remaining ingredients. 

 method: [sections] — * water -^ A, 24 to 48 hrs. -^ B, till differentiated -^ [counterstain] 



— > balsam, via usual reagents 

 note: A description of the use of this stain is given under 11.40 above. 



11.42 Lowit 1891 see ADS 22.1 Lowit 1891 



note: "Lowit's method" means only the use of his differentiating solution for any 

 safranin-stained material. 



11.42 Pfitzner 1881 14555,7:289 



formula: water 60, 95% ale. 40, safranin 0.3 



11.42 Rawitz 1895 Rawitz 1895, 76 



reagents rp:quired: A. 20% tannin; B. 2% potassium antimony tartrate; C. sat. sol. 



safranin; D. 2.5% tannin 

 method: [sections of chrome-fixed or mordanted material] -^ water -^ A, 24 hrs. -^ B, 



24 hrs. — * rinse, D, 24 hrs. — > water, till no more color comes away — >• D, is further 



differentiation required — > balsam, via usual reagents 



11.42 Roskin 1946 Roskin 1946, 152 



STOCK solution: water 50, 95% ale. 50, safranin 3.3 

 preparation: Dissolve the dye in ale, mix with water. 

 working solution: stock 20, 50% ale. 80 



11.42 Semichon 1920 test. 1934 Langeron Langeron 1934, 500 



formula: wat(M- 50, 90% ale. 50, safniniii 0.5, 40% formaldehyde 1 

 preparation: Dissolve dye in ale. then add other ingredients. 



