DS 11. 42-DS 11.431 DYE STAINS OF GENERAL APPLICATION 315 



method: [sections] -^^ 70% ale. —> stain, 30 niins. to 2 days —» absolute alcohol, till 

 differentiated — > balsam, via xylene 



11.42 Zwaademaker 1887 23632,4:212 



KEAGENTS REQUIRED: A. 95% alcohol 50, safranin 1,5, sat. aq. sol. aniline 50; B. absolute 



ale. 

 PREPARATION OP A: Dissolve dye in ale., then add aniline. 



method: [sections of F 1600.0010 fixed material] -^ 95% ale. -^ A, overnight-^ B, till 

 differentiated -^ balsam, via xylene 



11.43 MAGENTA 



11431 Magenta as Dye 



11.431 Albrecht test. 1943 Cowdry Cowdry 1943, 17 



formula: water 100, 95% ale. 20, phenol 8, magenta 4 

 preparation: Dissolve the dye in phenol and ale, then add water. 



11.431 Augusta 1932 see DS 11.431 Ziehl 1882 (note) 



11.431 Biot 1901 see DS 11.431 Gallego 1919 (note) 



11.431 Davalos test. 1894 Kahlden and Laurent Kahlden and Laurent 1894, 89 



formula: water 100, abs. ale. 1, phenol 5, magenta 10 



preparation: Grind the dye with the ale, wash out mortar with 10 successive portions 

 of 5% phenol. 



11.431 Dupres 1935 11425,46:77 



STOCK formula: water 80, 95% ale. 20, magenta 0.75 

 preparation OF stock: Digest 48 hours at 40°C. Cool. Filter. 

 reagents required: ^4. sat. aq. sol. aniline 100, stock 5, acetic acid 1.5; B. ADS 22.1 



Dupres 1936 

 method: [sections] -^ water —> A, 1-10 mins. — > B, till differentiated, 5-10 mins. — » 



counterstain -^ balsam, via usual reagents 



11.431 Gallego 1919 21344, 17:95 



REAGENTS REQUIRED: A. DS 11.43 Ziehl 2, water 98; B. 0.5% formaldehyde 

 method: [sections of formaldehyde material]-^ A, 5 mins. — » wash -^ B, 5 mins. -^ 



wash -^ balsam, via usual reagents 

 results: nuclei, dark violet, cytoplasmic structures, polychrome 

 NOTE : This method of converting magenta to a blue-black stain was originally proposed 



for bacteriological purposes by Biot 1910 (6593, Congres de Lyon, 234). 



11.431 Goodpasture and Burnett 1919 14975, 13:177 



formula: water 80, 95% ale. 20, phenol 1, aniline 1, magenta 0.5 

 preparation: Dissolve the dye in the mixture of ale. phenol and aniline. Add water. 

 note: This solution was republished by Goodpasture 1925 (608b, 1 :550) to which paper 

 and date reference are frequently made. See also DS 23.12 Hertig and Wolbach 1924. 



11.431 Henneguy 1891 11024, 27:397 



reagents required: A. 1% potassium permanganate; B. 1% magenta 



method: [sections or smears from F 1600.0010 Flemming 1882 fixed material] — » water 



— > A, 5 mins. -^ water, wash—* B, 5 mins. -^> water, rinse—* 95% ale., till partly 



differentiated — >• clove oil, till differentiation complete -^ balsam, via xylene 

 note: Schneider 1922, 113 applies this method to any section by mordanting in 1% 



chromic acid. A description of the use of this stain for chromosomes is given under 



DS 11.40 above. 



11.431 Huntoon 1931 591b, 1:317 



formula: water 75, glycerol 25, phenol 3.75, magenta 0.6 



11.431 Kinyoun test. 1946 Conn et al. Conn et al. 1946, 1 V, 6 



formula: water 100, 95% ale. 20, phenol 8, magenta 4 



