31G METHODS AND FORMULAS DS 11. 431-DS 11.44 



11.431 Krajian 1943 11284,28:1602 



formula: xylene 65, creosote 35, 95% ale. 5, magenta 0.3 

 preparation: Dissolve dye in ale. Add to other solvents. 



11.431 Manevall928 20540b, 4:21 



formula: water 90, aniline 2.5, 95% ale. 7.5, acetic acid 0.2, magenta 1 



11.431 Muller and Chermock 1945 11284,30:169 



formula: water 100, 95% ale. 20, phenol 8, magenta 4, w'etting agent 0.1 

 note: The original specifies "Tergitol 7" as the wetting agent. 



11.431 Pottenger 1942 test. Farber 1942 20540b, 17:183 



formula: water 80, 95% ale. 15, phenol 5, magenta 1.0 



11.431 Schneider 1922 see DS 11.431 Henneguy 1891 



11.431 Tilden and Tanaka 1945 Tech. Bull., 9:95 



formula: water 90, methanol 10, phenol 5, magenta III 0.5 



11.431 Ziehl 1882 7276, 8:451 and 1890 23632, 7:39 

 reagents required: A. water 100, 90% ale. 10, magenta 1, phenol 5; B. 1% acetic acid 

 preparation of a : Grind the dye with phenol in a mortar. When dissolved add the ale. 



in 10 successive lots while grinding. Then use water in 10 successive lots to wash out 



mortar. Filter accunmlated washings. 

 method: [sections or smears] -^ water -^ .1, 10-20 mins. -^ B, till differentiated —> 



counterstain -^ balsam, via usual reagents 

 note: For the use of this reagent for bacteria see DS 23.212 Neelsen 1883, and DS 23.211 



Ziehl. A detailed description of the former use is given under DS 23.20 (Chapter 21). 



The solution of Augusta 1932 (6630, 111:719) contains 2 magenta but is otherwise 



identical. 



1 1 .432 Magenta as Leucobase 



11.432 Coleman 1928 20540b, 13:123 



formula: water 100, magenta 1, A^-hydrochloric acid 4, potassium metabisulfite 1, 



activated charcoal 0.25 

 method: Dissolve dye in water. Add metabisulfite and then acid. Leave 24 hrs. in dark. 



Add charcoal, shake 1 min. Filter. 



11.432 Feulgen and Rossenbeck 1924 23543, 135:203 



formula: water 100, magenta 0.5, N-1 hydrochloric acid 20, 10% sodium bisulfite 5 

 preparation: Raise water to boil. Add dye. Cool to 50°C. and filter. Add acid to filtrate 



and cool to room temperature. Add bisulfite solution, leave 24 hours in dark. 

 method: see DS 22.1 Feulgen and Rossenbeck 1924 



note: The widely quoted method of de Tomasi 1936 (20540b, 11:137) differs only in 

 adding 0.5 dry potassium metabisulfite in place of 5 10% sodium bisulfite. See com- 

 ments on sodium bisulfite in note to DS 11.113 Knower 1930. 



11.432 Lillie 1948 Lillie 1948, 143 



note: This differs from Coleman 1928 only in the substitution of sodium metabisulfite 

 for potassium metabisulfite. 



11.432 SchifE 1886 825,140:93 



formula: water 100, magenta 0.025, sulfur dioxide q.s. to decolorize solution 



11.432 de Tomasi 1936 see DS 11.432 Feulgen and Rossenbeck 1924 



11.44 THIAZINS 



11.44 Anonymous 1946 4349,26:13 



formula: water 75, glycerol 20, abs. ale. 5, toluidine blue 1, lithium carbonate 0.5 

 preparation: Dissolve dye and alkali in water, incubate 24 hours at 37°C. and return 

 volume to 75. Add other ingredients. 



11.44 Borrell see DS 11.44 Langeron 1908 



