DS 11.44 DYE STAINS OF GENERAL APPLICATION 317 



11.44 Cobin 1946 Tech. Bull, 7 :92 



formula: water 100, sodium phosphate, dibasic 2.94, potassium dihydrogen phosphate 



3.68, methylene blue 0.76 

 prepar.\tion: Heat the dye and sodium pliosphate, dibasic in 30 water on a water bath 

 for 30 minutes. Cool and add the acid phosphate dissolved in 70 water. 



11.44 Gabbett 1887 see DS 23.213 Gabbett 1887 



11.44 Gatenby and Cowdry 1928 see DS 11.44 Nicolle 1871 (note) 



11.44 Goodpasture test. Langeron 1934 Laugeron 1934, 498 



formula: water 100, methylene l^lue 0.25, potassium carbonate 0.25, acetic acid 0.75 

 prepar.\tion: Boil the dye with the carbonate 30 minutes under reflux. Cool. Add acid, 

 shake to dissolve ppt. Boil 15 minutes. Cool. Filter. 



11.44 Jadassohn Ust. 1928 Schmorl Schmorl 1928, 150 



formula: water 100, methylene blue 1, sodium borate 1 



11.44 Kingsbury and Johannsen 1927 Kingsbury and Johannsenl927, 45 



formula: water 90, abs. ale. 10, methylene blue 0.2, potassium hydroxide 0.005 



11.44 Kuhne test. 1904a Besson Besson 1904, 155 



formula: water 100, 95% ale. 30, sodium carbonate 1, methylene blue 0.5 



11.44 Kuhne test. 1904b Besson Besson 1904, 154 



formula: water 100, 95% ale 10, methylene blue 2, phenol 2 



11.44 Langeron 1908 1886. 12:135 



preparation: To 100 0.5% silver nitrate add 3% sodium hydroxide till no further ppt. 



is produced. \\'ash ppt. thoroughly by decantation and add to it 100 1% methylene 



blue. Boil 5 minutes. Cool. Filter. 

 use: see DS 13.13 Shortt 1918. 

 note: This is "Borrel's Blue." The method of preparation as stated by Langeron 1942, 



545, differs little from that of Laveran 1900 (6630, 52:549). 



11.44 Langeron 1934 Langeron 1934, 496 



stock solutio.\: water 100, phenol 0.5, azur II 1 

 WORKING solution: water 90, stock 10, 1% potassium carbonate 2 

 method: [fixed smear] -^ stain, 1 min. -^ water, rinse — > dry -^ neutral mountant 

 note: This is also used as a mordant for some DS 13.12 techniques. 



11.44 Langeron 1942 Langeron 1942, 610 



reagents required: A. water 100, polychrome methylene blue 1; B. glyceric ether 25, 



water 75 

 method: [water] — > A, 5 min. to 12 hrs. -^ B, till differentiated -^ water, thorough wash 



— > abs. ale. least possible time -^ balsam, via cedar oil 

 note: Langeron {loc. cit.) also recommends Beauverie and Hollande 1916 ADS 22.1 in a 



1% dilution for B, above. 



11.44 Largret and Aubertin 1938 see 23.12 Largret and Aubertin 1938 



11.44 Laveran 1900 see DS 11.44 Langeron 1908 



11.44 Lbffler 1890 23684, 7:625 



formula: water 80, 95% ale. 20, methylene blue 0.3, potassium carbonate 0.8 

 preparation: Dissolve dye in ale. Add carbonate solution. 

 method: see DS 11.423 SahU 1885 



note: a description of a use for this stain in bacteriology is given under DS 23.20 in 

 Chapter 21. 



11.44 Manson test. 1929 Wenrich McClung 1929, 408 



formula of stock solution: water 100, sodium borate 5, methylene blue 2 

 PREPARATION OF STOCK SOLUTION: Stir the dye into the boiling borax solution. Cool. 

 FUter. 



