318 METHODS AND FORMULAS DS 11.44 



WORKING solution: water 100, stock 1 

 method: as DS 11.44 Langeron 1934 



note: When used after DS 13.12 Wright, or similar stains, the term panoptic is some- 

 times applied. 



11.44 Manwell 1945 11284,30:1078 



formula: water 100, methylene blue 0.1, 1% sulfuric acid 0.6, potassium dichromate 



0.1, 1% potassium hydroxide 2 

 preparation: Dissolve dye in water. Add acid and dichromate. Autoclave 2 hours at 



3 pounds or till solution is blue. Add alkali drop by drop shaking till ppt. dissolved. 



Leave 48 hours. Filter. 

 NOTE : Manwell (loc. cit.) states this method to be a modification of that of Singh, Jaswant 



and Bhattacharji 1944 (9943, 79:102), and refers to it as the "JBS" {sic) method. 



11.44 Martinotti 1910 23632, 27:24 



formula: water 75, glycerol 20, 95% alcohol 5, toluidine blue 1, lithium carbonate 0.5 



11.44 Michaelis 1901 23684, 29:763 



formula: water 90, methylene blue 1, 0.4% sodium hydroxide 5, 0.5% sulfuric acid 5 

 preparation: Add the alkali to the solution of the dye, boil 15 minutes. Cool. Add acid, 



filter. 



11.44 Moschkowsky test. 1946 Roskin Roskin 1946, 287 



formula: water 100, sodium borate 2, methylene blue 1 



11.44 MuUer and Chermock 1945 11284, 30:169 



formula: water 70, 95% ale. 30, potassium hydroxide 0.0007, methylene blue 0.44 



11.44 Nicolle 1871 23730, 9 



formula: sat. sol. thionine in 50% ale. 10, 1% phenol 90 



note: Gatenby and Cowdry 1928, p. 187 use 50% ale. and increase the phenol solution 

 to a 6: 1 ratio. Langeron 1942, 539 takes 20 thionin solution to 80 2% phenol. Conn 

 1936 cites "Thionin, carbol-, Nicolle's" {sic) in the index but not in the text. 



11.44 Proescher and Drueger 11284, 10:153 



formula: a. water 100, methylene 1, sodium peroxide 0.025, hydrochloric acid q.s. 

 preparation: Boil dye and peroxide for 15 minutes. Cool. Adjust to pH 7 with hydro- 

 chloric acid. 



11.44 Roques and Jude 1940 test. 1949 Langeron Langeron 1949, 621 



preparation: Grind 1 methylene blue with 10 anhydrous potassium sulfate. Add 100 

 95% ale. and shake at intervals for some hours. Filter and evaporate filtrate to 

 dryness. 



11.44 Stoughton 1930 1025, 17:162 



formula: water 95, phenol 5, thionin 0.1 ^ 



11.44 Terry 1922 11284, 8:157 



formula: water 100, methylene blue 0.2, potassium carbonate 0.2 

 preparation: Boil 23^ minutes. Cool. 



11.44 Raadt 1912 see DS 13.12 Raadt 1912 



11.44 Sahli 1885 23632,2:14 



reagents required: A. water 70, sodium borate 30, sat. sol. (arc. 4%) methylene blue 1 

 method: [sections of chrome-fixed or mordanted material] —> water—* A, 1-3 hrs. — > 

 abs. ale. till differentiated — > balsam, via usual reagents 



11.44 Singh, Jaswant, and Bhattacharji 1944 see DS Manwell 1945 (note) 



11.44 Stevenel 1918 5310, 11:870 



formula: a. water 100, methylene blue 1.3, potassium permanganate 2.0 

 preparation: Dissolve the dye and permanganate each in 50 water. Mix and heat on 



water bath tiU ppt. redissolved. Cool. Filter. 

 method: see DS 13.13 Boy6 1940 



