DS 11.44 DS 12 



DYE STAINS OF GENERAL APPLICATION 



319 



11.44 Unna 1892 23632,7:483 



PREPARATION OF DRY STOCK: Water 100, 95% ale. 20, methylene blue 1, potassium 



carbonate 1. 

 preparation: Simmer ingredients till volume reduced to 100. Leave 24 hours. Filter. 



Evaporate to dryness. 



11.44 Volkonsky 1933 test. 1942 Langeron Langeron 1942, 1105 



formula: water 50, glycerol 50, methylene violet 0.4, azur II 0.1, potassium carbonate 

 0.1 



11.45 CRYSTAL VIOLET 



11.45 Atkins 1920 1105G, 5:321 

 formula: water 75, 95% ale. 25, aniline sulfate 0.75, crystal violet 3.8 



11.45 Brown and Brenn 1931 10919, 48:69 



formula: water 100, phenol 0.1, sodium bicarbonate 1.25, crystal violet 0.75 



11.45 Huntoon 1931 591b, 1:317 



formula: water 60, glycerol 25, 95% ale. 15, crystal violet 0.45 



11.45 Johansen 1932 20540b, 7:17 



REAGENTS REQUIRED: A. water 100, 95% ale. 20, crystal violet 1; B. 1% picric acid in 



95% alcohol 

 method: [sections, perfectly freed from fixative] —> water —> ^4, 5 mins. — > water, rinse 



-^ B, till no more color comes away —* balsam, via usual reagents 

 note: The author strongly recommends this for nuclei in heavily yolked material. 



11.45 LaCour 1931 11360, 51:124 



reagents required: A. 1% gentian violet; B. ADS 12.2 Lugol (1905); C. 1% chromic 



acid 

 method: [sections or smears] — > water -^ A, 10 mins. — > water, quick rinse — » abs. ale, 



2 sec. — » 5, 2 mins. -^ abs. ale, 2 sec. —> C, 15 sec. — > abs. ale, 5 sec. — > C, 15 sec. — » 

 abs. ale, 15 sec. -^ clove oil, till clear — » xylene, till clove oil removed — + balsam 



11.45 Nastikow test. 1904 Besson Besson 1904, 155 



formula: water 100, gentian violet 0.2, mercuric chloride 0.05 



11.45 Newton 1927 11295,47:346 



reagents required: A. 1% gentian violet 100; B. ADS 12.2 Lugol (1905) 

 method: [sections or smears] — > water -^ A, 3-10 mins. — * water, rinse — > B, 30 sees. — > 

 95% ale, quick rinse — > abs. ale, 5-10 sees. — > clove oil, till differentiated —> xylene, 



3 changes, till clove oil removed — > balsam 



11.45 Stirling 1890 test. Mallory 1938 Mallory 1938, 90 

 formula: water 88, 95% ale 10, aniline 2, gentian violet 5 



11.46 OTHER SYNTHETIC NUCLEAR STAINS 



11.46 Lendrum 1945 11431, 57:267 

 formula: water 90, 95% ale 10, dextrin 0.5, phenol 0.4, acid fuchsin 1 

 preparation: Mix the dye with the molten phenol and add the ale. Add to this the 



dextrin dissolved at 80°C. 



12 PLASMA STAINING TECHNIQUES 



Plasma staining techniques serve a dual 

 role. Originally intended only to act as a 

 contrast to the nuclear staining of the 

 color selected, they may also be adapted 

 to a wide range of differential tissue stain- 

 ing. Indeed, there is a very poor case to be 

 made for their use unless they are so em- 

 ployed, for a good hematoxylin or oxazine 



nuclear stain requires no contrast which 

 cannot be more simply and readil}^ pro- 

 vided by a color screen between the lamp 

 and the microscope than by staining the 

 section examined. The techniques here 

 given are divided into two main divisions: 

 first, those which give only a single color 

 irrespective of the material on which they 



