DS 12.15-DS 12.16 DYE STAINS OF GENERAL APPLICATION 321 



method: [sections, or smears, with desired prior staining] —» 95 % ale. ^ stain, usually 

 dropped on slide, till sufficiently colored —> clove oil, dropped on slide, till excess stain 

 removed -^ xylene, several changes — + balsam 



note: The reputation for rapid fading, which stains applied in this manner enjoy, is largely 

 due to imperfect removal of clove oil before mounting. 



12.15 PHENOL SOLUTIONS, compl. script, (but probably originated by Ziehl 1882 7276, 



8:451) 



formula: water 100, phenol 2, 95 f'^ ale. 10, cresyl violet 1 or instead of cresijl violet, either 

 gentian violet 1 (= "Gram's violet" — and.) or methylene blue 1.5, or magenta 1 (see 

 also DS 11.43 Ziehl 1890), or thionin 1.5 (see also DS 11.46 Nicolle), or isamine blue 1 

 (see DS 23.11 Nicolau 1939), or crystal violet 1 (see DS 23.213 Carpano 1910), or crystal 

 violet 2 (see DS 23.213 August 1932), or rose bengal (see DS 23.211 Conn 1928) 



prepar.\tion: Grind the dye with the phenol and ale. in a mortar. Wash out the mortar while 

 grinding with 10 successive portions of water. Filter the accumulated washings after 

 24 hours. 



12.16 OTHER SOLUTIONS 



12.16 Bensley 1916 590, 29:37 

 FOR.MUL.^: water 100, anilin blue 0.2, phosphomolybdic acid 1 



method: [sections with red nuclei]-* waters stain, 5 mins. — > water, quick rinse —> 



-^ abs. ale, till differentiated — >• balsam, via xylene 

 note: This was originally designed by Bensley {loc. cit.) to follow his DS 11.3 brazilin. 



12.16 Donaldson 1917 iodine-eosin 11995,192:571 



formula: ads 12.21 Lugol 1905, 50, sat. aq. sol. eosin Y 50 



method for protozoans in fece,s: Mix 10 parts stain with 1 feces — > place under cover- 

 slip -^ seal 

 note: This method is also applica])lc to many marine invertebrate larvae. 



12.16 Fraenkel test. 1948 Romeis tannin-fuchsin Romeis 1948, 519 



formula: water 60, acid fuchsin 0.12, tannin 10, ADS 22.1 Unna (1928) 33 



12.16 Jensen 1937 23684, 39:333 



formula: DS 11.24 Jensen 1937 (stock II) 100, phenol 0.5, eosin B 0.05 



12.16 Johansen 1940 fast green Johansen 1940, 59 



formula: methyl cellosolve 30, clove oil 30, abs. ale. 30, fast green FCF 0.5 

 note: Though intended for botanical histology (see DS 21.41 Johansen 1940) this solu- 

 tion is of wide application as a counterstain. 



12.16 Kofoid (1920) see DS 23.33 Kofoid (1920) 



12.16 Krajian 1938 eosinol—aud. 1887a, 25:376 



prep.\ration of dry stain: To 0.35 eosin B dissolved in 0.7 water add 0.7 acetic acid. 



Mix and incubate at 36°C. until dry. 

 working solution: xylene 75, phenol 25, all dry stain from above 



12.16 Maneval 1936 20540b, 11 :9 



formula: water 80, acetic acid 20, anilin blue 0.01 {or, instead of anilin blue, acid 

 fuchsin 0.1) ferric chloride 0.6 



12.16 McClean 1934a New Phytologist, 33 ilUi) 



PREPARATION OF DRY STOCK: To 50 1 % eosin add 1.75 10% hydrochloric acid. Leave 24 



hours, filter, dry ppt. 

 WORKING solution: xylene 100, all ppt. from above 

 note: Erythrosiii may be substituted for eosin. 



12.16 McClean 1934b New Phi/tologisl, 33 •:il(i 



preparation of dry stock: To 50 1% nile blue sulfate add 12.5 10% sodium hydroxide. 



Leave 24 hours, filter, dry ppt. 

 wOKKixc; solution: xylene 100, all ppt. fttim al)()ve 



12.16 Nuttall 1908 16035,1:162 



formula: xylene 100, picric acid to sat. {circ. 10%) 

 note: Originally intended for potash-cleared arthropod endoskeletons. 



