326 METHODS AND FORMULAS DS 12.211 



12.211 Grosso 1914 picro-methyl green 8545,18:71 



PREPARATION OF DRY STOCK: Add a sat. aq. sol. picric acid to a sat. aq. sol. methyl green 



till no further ppt. is formed. Wash and dry ppt. 

 PREPARATION OF STOCK SOLUTION: methanol 100, dry stock 0.5 

 PREPARATION OF WORKING SOLUTION: stock solution 15, Water 65 



method: [sections with red nuclei] -* stain, 5-10 mins. —>• water, quick rinse —> abs, 

 ale, till red color clouds cease -^ balsam, via xylene 



12.211 Grosso 1914 see DS 21.3 Grosso 1914 



12.211 Hruby 1933 see DS 22.12 Hruby 1933 



12.211 Krause 1911 test. 1948 Romeis picro-indigo-carmine 



Romeis 1948, 169 

 formula: water 100, picric acid 1, indigo-carmine 0.3 



12.211 Lillie 1945 picro-blue 11571b, 25:1 



formula: water 100, picric acid to sat., methyl blue 0.1 or anilin blue 0.1 

 note: Lillie 1948 p. 191 recommends this as a contrast for blue nuclei. 



12.211 Lillie 1948 picro-naphthol black Lillie 1948, 191 



formula: water 100, picric acid q.s. to sat., naphthol blue black 0.02 to 0.04 



12.211 Masson test. 1934 Langeron picro-indigo-carmine 



Langeron 1934, 552 

 REAGENTS REQUIRED: A. 1% acetic acid; B. sat. aq. sol. picric acid 100, indigo-carmine 



0.25; C. 0.2% acetic acid 

 method: [nuclei red, preferably by some DS 11.43 method] -^ A, thorough rinse —> B, 

 10 mins. — » water, quick rinse -^ C, till connective tissue clear blue, about 2 mins. — > 

 abs. ale. shortest time to complete dehydration — > balsam, via xylene 

 result: very like Cajal 1895 but with a greater range of shades. 



12.211 Minchin test. 1928 Goodrich picro-light green Gatenby and Cowdry 1928, 432 

 formula: 90% ale. 100, picric acid 5, light green 1 

 method: [red nuclei] —> 90% ale. — » stain, 10 mins. — > abs. ale. tUl differentiated—* 



balsam, via xylene 

 result: similar to Smith 1912. 



12.211 Neubert 1922 picro-thiazin red 23418(1), 66:424 



reagents required: A. water 100, 95% ale. 10, thiazin red 0.15, picric acid 0.03; 



B. sat. 95% ale. sol. picric acid 

 method: [sections with hematoxylin-stained nuclei]-^ water —> A, till collagen deeply 



stained — > rinse -^ B, thorough wash -^ balsam, via usual reagents 



12.211 Pfitzer 1883 picro-nigrosin 2626, 1 :44 



formula: sat. sol. picric acid 100, nigrosin 0.2 



12.211 Pol 1908 test. 1948 Romeis picro-indigo-carmine 



Romeis 1948, 169 

 formula: water 100, picric acid 1, indigo-carmine 0.4 



12.211 Roskin 1946 see DS 13.43 Roskin 1946 



12.211 Shumway 1926 picro-indigo-carmine see DS 13.5 Shumway 1926 



12.211 Smith 1912 picro-spiril blue 11373,23:94 



formula: sat. ale. sol. {circ. 1%) spirit blue 100, picric acid 1 

 method: [sections of material bulk stained in carmine] — ♦ abs. ale. -^ stain, 2 mins. — > 



abs. ale, till differentiated — > balsam, via xylene 

 result: nuclei, red; yolk, yellow green; yolk-free cytoplasm, clear blue. 



12.211 White test. 1905 Hall and Herxheimer picro-erythrosin 



Hall and Herxheimer 1905, 63 

 formula: water 100, sat. ale. sol. erythrosin 4, picric acid 0.6, calcium carbonate to 

 excess 



