328 METHODS AND FORMULAS DS 12.221-DS 12.222 



method: [blue nuclei (DS 11.123 Ehrlich 1886 specified in original)]—* waters stain, 

 1-2 mins. -^ blot — > abs. ale, minimum time for dehydration -^ balsam, via usual 

 reagents 



result: muscle, red; other tissues, yellow and orange. Good for most heavily muscular- 

 ized tissues. 



12.221 Hansen 1898 picro-fuchsin 766,15:152 



REAGEXTs required: A. sat. sol. {circ. 1.2%) picric acid 100, acetic acid 0.3, acid 



fuchsin 0.1; 5. water 98, A. 2 

 method: PdIuc nuclei] — > water —* A, some hrs. — * B, wash -^ balsam, via xylene 

 result: selective red stain on white fibrous connective tissue. 



12.221 Lillie 1948 picro-fuchsin Lillie 1948 



formula: water 100, picric acid 1, acid fuchsin 0.1, hydrochloric acid 0.25 

 note: Lillie (loc. cit.) also recommends his DS 12.211 solution as a contrast for hema- 

 toxylin-stained nuclei. 



12.221 Ohlmacher 1897 picro-fuchsin 11189,2:675 



formula: sat. sol. {circ. 1.2%) picric 50, water 50, acid fuchsin 0.5 



12.221 Schaffer 1899 picro-fuchsin 23635,66:214 



formula: sat. aq. sol. picric acid, acid fuchsin 0.15, acetic acid 0.05 



12.221 Thompson 1945 see DS 21.3 Thompson 1945 



12.221 Unna test. Lillie 1948 picro-fuchsin Lillie 1948, 190 



formula: water 90, acid fuchsin 0.25, nitric acid 0.5, glycerol 10, picric acid q.s. to sat. 



12.221 Weigert 1904 picro-fuchsin 23632, 21 :3 



formul.^: sat. sol. {circ. 1.2%) picric acid 100, acid fuchsin 0.1 

 method: as van Gieson above 



12.221 Wilhelmini 1909 picro-fuchsin 8338,22:18 

 formula: water 90, 95% ale. 10, ammonium picrate 0.8, acid fuchsin 0.2 

 method: as van Gieson above 



12.322 Other Formulas 



12.222 Delephine test. circ. 1938 Wellings fuch sin-orange 



Wellings 1938, 104 

 formula: water 100, acid fuchsin 0.04, orange G 0.2 



12.222 Gray 1952 ponceau-orange Gray 1952, 24 



formula: water 100, orange II 0.6, ponceau 2R 0.4 



method: Palue nuclei] — > water — >■ stain, 1-2 mins. -^ blot -^ abs. ale, till differentiated 

 —* balsam, via usual reagents 



12.222 Gregg and Puckett 1943 eosin-orange 20540b, 18:179 



formula: 95% ale. 100, eosin 0.2, orange G 0.01 



12.222 Guyler 1932 indigo-carminc-eosin Y 11977,18:314 



formula: water 100, indigo-carmine 0.25, eosin Y 1, thymol trace 

 method: [blue nuclei (original specifies 11.122 Delafield 1885)]-^ water— > stain, over- 

 night — > water, quick rin-se — > abs. ale, till differentiated — >• balsam, via usual 

 reagent 



12.222 Hayem test. 1896 Kahlden and Laurent eosin-aurantia 



Kahlden and Laurent 1896, 117 

 formula: 1% eosin W, 1% aurantia, a.a. q.s. to give rose-colored solution 



12.222 Kingsbury and Johannsen 1927 orange-acid fuchsin 



Kingsbury and Johannsen 1927, 76 

 formula: water 100, glj'cerol 7, orange G 1, acid fuchsin 2 



12.222 Langeron 1942 see DS 12.222 Masson 1911 (note) 



