DS 12.31 DYE STAINS OF GENERAL APPLICATION 337 



12.31 Goldner 1938 acid fuchsin-ponceau 3R-oran<je G-light green 



608b. 14:237 

 REAGENTS REQUIRED: A. Water 100, ponceau 2R 0.07, ackl fuchsin O.Oii, acc^tic acid 0.2; 



B. 1% acetic acid; C. water 100, phosphomolybdic acid 4, orange G 2; D. water 100, 



light green 0.2, acetic acid 0.2 

 method: [sections with nuclei stained by DS 11.121 Weigert 11)01] — > thorough wash — » 



A, 5 mins. -^ B, wash — > (!, till collagen decolorized -> H, rinse — ♦ D, 5 niins. —* H, 



5 mins. — > blot — > abs. ale, least possible time — ♦ balsam, via xylene 

 result: general cytoplasm, red; erythrocytes, orange; collagen, green. 

 note: Other color combinations may be obtained by substituting for A ai)ove either 



water 100, azophloxin 0.5, acetic acid 0.2 or water 100, acid fuchsin 0.025, ponceau 



2R 0.075, azophloxine 0.01, acetic acid 0.2. For a further modification see I^S 13.7 



Romcis 1948. 



12.31 Haythorne 1916 acid fuchsin-orange G-anilin blue 



4349, 6 :61 

 REAGENTS REQUIRED: A. water 100, hydrochloric acid 0.06, 95% ale. 4, orange G 0.8, 



ferric alum 5; B. 0.5% acid fuchsin; C. sat. aq. sol. phosphomolybdic acid 100, anilin 



blue 2.5, orange G 2.5 

 preparation of A: Dissolve the orange G in 70 water with the ale. and acid. Dissolve 



the alum in 25 water and add to the dye solution. Filter. 

 method: [sections of F 3700.0010 Zenker 1894 fixed material after 30 mins. staining in 



DS 11.122 Bohmer 1868] -> water — >• A, 2 mins. -^ water, 5 mins. -^ B, 3 mins. -^ 



blot —> C, 20 mins. —> blot — » 95% ale, quick rinse —> abs. ale, from drop bottle, till 



differentiated — > balsam, via xylene 

 result: nuclei, reddish black; cartilage, white fibrous tissue, blue; keratin, chitin, 



erythrocytes, bright orange; muscle, red. 



12.31 Laidlaw see DS 23.11 Laidlaw (1936) 



12.31 Lendrum and McFarlane 1940 picro-orange G-acid fuchsin-ponceau 2R-anilin blue 



11431, 50:381 

 reagents required: A. water 20, 95% ale. 80, picric acid 1, orange G 0.2; B. water 99, 

 acetic acid 1, acid fuchsin 0.5, ponceau 2R 0.5, sodium sulfate 0.25; C. 1% acetic 

 acid; D. 1% phosphomolybdic acid; E. water 99, acetic acid 1, anilin blue 2 

 method: [.sections with nuclei stained by DS 11.41 technique] — > water — * A, 2 mins. — » 

 overnight — > rinse — > B, 1-5 mins. — > C, rinse — > D, till collagen not quite decolorized 

 — > E, 2-10 mins. -^ C, rinse -^ balsam, via usual reagents 

 note: Fast green FCF may be substituted for anilin blue in E above. 



12.31 Lillie 1940 Biebrich scarlet-fast green 20540b, 15:21 



reagents required: A. water 99, acetic acid 1, Biebrich scarlet 1; B. water 100, phos- 



photungstic acid 2.5, phosphomolybdic acid 2.5; C. water 97.5, acetic acid 2.5, fast 



green FCF 2.5; D. 1% acetic acid 

 method: [sections with blue nuclei (original specifies DS 11.121 Weigert 1903)] — > water 



— > A, 2 mins. — > rinse — > B, 1 min. -» C, 2 mins. -» Z), 1 min. or till differentiated -^ 



balsam, via acetone and xylene 



12.31 Masson 1912a acid fuchsin-anilin blue 4956,87:290 



reagents required: A. water 100, acetic acid 0.5, acid fuchsin 0.5; B. 1% phospho- 

 molybdic acid; C. water 100, acetic acid 2.5, anilin blue to sat.; D. 1% acetic acid; E. 

 0.1% acetic acid in abs. ale. 



method: [sections (original required prior staining in DS 11.111 R6gaud 1910)] —^ water 

 —* A, 5 mins. -^ water, quick rinse — » B, 5 mins. -^ drain -^ C, poured on slide, 2-5 

 mins. -^ D, till differentiated 5-30 mins. — > E, till dehydrated -^ salicylic-xylene —>■ 

 salicylic balsam 



result: nuclei, black (if prior stained in Rdgaud) or deep red ; coUagens, light blue; bone, 

 dark blue; epithelia, muscle, some glands, light red; erythrocytes, orange; nervous 

 tissues, violet. 



note: a detailed description of the use of this stain is given under 12.30 above. 



