338 METHODS AND FORMULAS DS 12.31 



12.31 Masson 1912b acid fuchsin-ponceau 2R-anilin blue 



4956, 87 :290 

 REAGENTS REQUIRED: A. water 100, acetic acid 1, acid fuchsin 0.35, ponceau 2R 0.65; 



B, C, D, E as Masson 1912a above 

 method: as Masson 1912a above 



12.31 Masson 1912c acid fuchsin-metanil yellow 4956, 87 :290 



REAGENTS REQUIRED: A. Water 1, acetic acid 1, acid fuchsin 100; B. 1% phosphomolyb- 



dic acid; C. sat. sol. {circ. 6%) metanil yellow; D. 1% acetic acid 

 method: [sections (original required prior staining in DS 11.111 Regaud 1910)] — >• water 

 -^ A, 5 mins. — > water, quick rinse -^ B, 5 mins. — > drain —> C, poured on .slide, 5 

 mins. -^ D, 5 mins. -^ salicylic balsam, via usual reagents 



12.31 Masson 1912 see cdso DS 12.32 Masson 1912 and DS 13.41 Masson 1912 



12.31 McFarlane 1944a picro-acid fuchsin-anilin blue 20540b, 19:29 



reagents required: A. water 98, acetic acid 2, picric acid 0.2, phosphotungstic acid 1, 

 acid fuchsin 1, anilin blue 2; B. 2% acetic acid; C. water 90, 95% ale. 10, picric acid 

 0.25, phosphotungstic acid 2.5 

 method: [sections with blue nuclei] -^ water ^ ^, 5 mins. — > B, rinse — > C, till differ- 

 entiated — > D, 1 min. -^ B, wash -^ balsam, via usual reagents 



12.31 McFarlane 1944b picro-acid fuchsin-anilin blue 20540b, 19 :23 



reagents required: A. water 98, acetic acid 2, acid fuchsin 0.8, picric acid 0.2; B.2% 

 acetic acid; C. water 60, 95% ale. 40, picric acid 1, phosphotungstic acid 10; D. water 

 97.5, acetic acid 2.5, anilin blue 2.5; E. water 90, 95% ale. 10, picric acid 0.25, phos- 

 photungstic acid 2.5 

 method: [sections with DS 11.121 stained nuclei] — » water — > A, 5 mins. — » B, rinse — * 



C, 5 mins. -^ rinse — > D, 5-10 mins. — > B, rinse — > £", 5 mins. -^ B, wash — » balsam, 

 via usual reagents 



12.31 McFarlane 1944c picro-orange G-acid fuchsin-ponceau 2R-anilin blue 



20540b, 19:23 



reagents required: A. water 20, 95% ale. 80, picric acid 1, orange G 0.25; B. water 99, 

 acetic acid 1, acid fuchsin 0.25, ponceau 2R 0.25; C. 2% acetic acid; D. water 20, 

 95% ale. 80, picric acid 1, phosphotungstic acid 10; E. water 97.5, acetic acid 2.5, 

 anilin blue 2.5; F. water 80, 95% ale. 20, picric acid 0.5, phosphotungstic acid 5 



method: [sections, stained but not differentiated, in DS 11.111 Regaud 1910] —> rinse 

 — » A, till nuclei differentiated -^ wash, till only erythrocytes yellow -^ B, 5-10 mins. 

 -^ C, rinse — > Z), 5 mins., till differentiated —>■ C, rinse — > E, 10 mins. — > C, rinse -^ F, 

 till differentiated -^ C, wash -^ balsam, via usual reagents 



12.31 Papanicolaou 1941 see DS 23.4 Papanicolaou 1941 



12.31 Pasini test. 1928 Hill eosin B-acid fuchsin-anilin blue-orcein 



Gatenby and Cowdry 1928, 280 

 reagents required: A. 2% phosphotungstic acid; B. 50% ale. 35, glycerol 40, eosin B 



0.7, DS 12.216 Unna (1928) 35, sat. aq. sol. acid fuchsin 4 

 preparation of b: Dissolve the eosin in 35 50% ale. Add, in order, the Unna's stain, 



fuchsin solution, and glycerol. 

 method: [sections with blue nuclei (original specifies DS 11.123 Ehrlich 1886)] —* water 



— > A, 10 mins. — *• water, quick rinse -^ B, 15-20 mins. — > 70% ale. quick rinse —> 



abs. ale, 20 sees. -^ A, 5 sees. -^ abs. ale. till differentiated -^ balsam, via xylene 

 result: coUagen, blue; elastic fibers, purple; erythrocytes, bright orange. 

 note: See also DS 13.42 Walter 1930. 



12.31 PoUak 1944 orange G-light green-ponceau 2R-acid fuchsin 



1887a, 37 :294 

 reagents required: A. water 50, 95% ale. 50, acetic acid 1, phosphotungstic acid 0.5, 

 phosphomolybdic acid 0.5, orange G 0.25, light green SF 0.15, ponceau 2R 0.33, acid 

 fuchsin 0.17; B. 0.2% acetic acid 



