DS 12.31-DS 12.32 DYE STAINS OF GENERAL APPLICATION 339 



PREPARATION OF A'. Mix Water, ale, and acetic acid. Divide into four portions. In first 

 dissolve ))h()si)honiolyb(lic acid with licat; in second dissolve pliosphotiingstic acid 

 and Orange G; in third dissolve light green; in fourth dissolve acid fuchsin and 

 ponceau 2R. Mix and filter. 



method: [sections with blue nuclei] -^ water -^ A, 3-7 mins. —> B, till differentiated — + 

 95% ale, till dehydrated — > balsam, via usual reagents 



12.31 Wallart and Honette 1934 (iri<l furhKin-Ja.sl ijcUow 



4285a, 10:404 

 REAGENTS REQUIRED! A. \% acid fuchsin in 1% acetic acid 30, 3% fast yellow in 1% 

 acetic acid 30, 1% phosphoniolybdic acid 30; B. 1% acetic acid; C. 1% acetic acid in 

 abs. ale. 

 method: [sections with nuclei stained in DS 11.121 Wcigcrt 1903] — * tap water—* A, 5 

 mins. — * quick rinse -^ B, 5 mins. — > C, dropped on from pii)et, 30 sees. — > abs. ale. —* 

 xylene — > balsam 

 result: black nuclei, red cytoplasm, yellow collagen, pink clastin. 



12.31 Weiss 1932 see DS 12.31 Brillmeyer 1929 (note) 



12.32 TECHNIQUES EMPLOYING THE PHOSPHOTUNGSTIC (-MOLYBDIC-) REACTION 



WITH OTHER DYES 



12.32 Dupres 1935 toluidine blue-orange G 14425,46:46 



REAGENTS REQUIRED: A. 1% phosphomolybdic acid; B. water 100, toluidine blue 0.25, 



orange G 4, oxalic acid 4 

 method: [red nuclei (original specifies DS 11.43 Dupres 1935)] -^ A, 10 mins. -^ water, 



thorough wash — > B, 2-5 mins. — > drain -^95% ale. till differentiated —^ balsam, via 



usual reagents 

 result: nuclei, red; collagens, blue; bone, dark green; muscle, light green; erythrocytes, 



bright orange; epidermis, blue; layer of Malpighi, orange; hair, etc., bright red. 

 note: Dupres 1935 {loc. cil.) also recommends methyl green in place of toluidine blue in 



B above. 



12.32 Gomori 1950 chromolrope-fast green Tech. Bull, 20:77 



reagents required: A. water 100, acetic acid 1, chromotrope 2R 0.6, fast green FCF 



0.3, phosphotungstic acid 0.6; B. 0.2 acetic acid 

 method: [smears, or sections not more than 5 /i thick, prior stained in hematoxylin] — > 



water — > A, 5-20 mins. —> B, rinse — > balsam, via usual reagents 

 note: Wheatley 1951 {Tech. Bull., 21:92) recommends this technique for protozoans in 



intestinal smears. 



12.32 Hollands 1912 magenta-orange G-lighi green 1915,10:62 



reagents required: ^4. 1% magenta in 70% ale; B. 0.1% hydrochloric acid in 70% 

 ale; C. 1% phosphomolybdic acid; D. sat. sol. {circ. 11%) orange G; E. 0.2% light 

 green 



method: [blue nuclei (Langeron 1942, 606 recommends DS 11.122 methods)] — » water — > 

 A, 6-12 hrs. — > water, 5 mins. -^ B, till color clouds cease, few seconds -^ water, 

 thorough wash -^ C, 5 mins. -^ water, rinse — > D, 5 mins. — > E, poured on slide 3*^ to 

 1 min. -^ 95% ale, few sees., till differentiated —* balsam, via amyl ale and benzene 



result: resting nuclei, blue; mitotic figures, red; cartilage, purple; fibrous tissue, light 

 green; erythrocytes and keratin, bright orange. 



12.32 Koneff 1936 see DS 13.7 Koneff 193G 

 12.32 Lillie 1940 see DS 12.31 Lillie 1940 



12.32 Masson 1912 ponceau 2R-anilin blue 4956, 87 :290 



reagents required: A. water 100, acetic acid 1, ponceau 2R 1; B; C; I); E, as DS 12.31 



Masson 1912a 

 method: as DS 12.31 Masson 1912a 



12.32 Patay 1934 ponceau 2R-light green 4285a, 11:408 



reagents required: A. 1% ponceau 2R; B. 1% phosphomolybdic acid; C. 0.5% liglit 

 green in 90% ale 



