DS 13.11-DS 13.12 DYE STAINS OF GENERAL APPLICATION 345 



13.11 Lim 1919 17510, 63:542 



REAGENTS REQUIRED: A. 1% cthyl eosin in abs. ale; B. \% methylene blue 



method: [sections or smears] — > abs. ale. — > ^1, on slide, 1 min. -^ water, thorough wash 



—* B, on slide, 1 min. -^ blot — + abs. ale., flooded on slide, 2-3 sees. -^ benzene — > 



neutral mountant or — » dry 



13.11 May-Griinwald 1902 23720, 11:265 



PREPARATION OF DRY EosiNATE: Mlx cqual parts of 0.5% eosin Y and 0.5% methylene 

 blue. Filter. Dry filtrate. Wasli dried filtrate and redry. 



WORKING SOLUTION: methanol 100, dry powder from above to sat. 



method: [air-dried smear] —> stain, on slide, 3 mins. ^ add equal volume distilled 

 water, leave 1 min. — » wash — > dry 



note: See note under Jenner 1899 with which this technique is nearly, but not quite, 

 identical. Zieler {Icsl. Schmorl 1928, 2-16) stains paraffin sections 2-3 minutes and de- 

 hydrates in acetone; for another application of this stain to sections see DS 13.11 

 Assmann 1906b above. 



13.11 Michaelis 1901 7276,27:127 



formula: water 40, abs. ale. 25, acetone 35, methylene blue 0.25, eosin 0.15 

 preparation: Dissolve the methylene blue in 25 water, 25 ale. Dissolve the eosin in 15 

 water, 35 acetone. Mix. Filter. 



13.11 Miiller test. 1928 Schmorl Schmorl 1928, 239 



reagents required: A. 0.5% eosin Y in 70% ale.; B. A 60, 0.25% methylene blue 30 

 method: [blood smear fixed 3 mins. in methanol]-^ A, 3-5 mins. — > distilled water, 

 wash -^ blot — > B, J2-I min. -^ distilled water, wash -^ dry 



13.11 Nocht test. 1903 Ehrlich Ehrlich, Krauso, et al. 1903, 784 



formula: water 65, acetone 17, 0.1% thiazin 10, 0.1% eosin 10, buffer pH 4.6 

 note: This is not a specific technique, though it is often quoted as such; it is a general 

 direction for experiments with any thiazins and any eosin. 



13.11 Sabrazes 1911 6630,70:247 



reagents required: A. 30% eosin Y in 95% ale.; B. 0.2% methylene blue 

 method : [smear] — > place 1 drop A on smear and 1 drop B on coverslip — > seal with wax 

 for temporary examination -^ or water, quick rinse — * dry 



13.11 Willebrand 1901 7276, 27 :57 



formula: water 65, abs. ale. 35, eosin 0.25, methylene blue 0.5, 1% acetic acid q.s. 

 preparation: Dissolve the eosin in 35 ale, 15 water. Dissolve the methylene blue in 50 



water. Mix the solutions and add acid drop by drop till solution turns red. Filter. 

 method: [fresh smear] -^ stain 5 mins. -^ rinse -^ dry 



13.11 Zieler see DS 13.11 May-Griinwald 1902 (note) 



13.12 polychrome methylene blue-eo.sinates 



13.12 Brice 1930 see D. DS 22.8 Brice 1930 



13.12 Diercks and Tibbs 1947 11056, 53:479 



REAGENTS REQUIRED: A. Water 100, DS 13.13 MacNcal 1922 6 



method: [moist smear] —^ methanol 3 to 5 mins. -^ A, 15-20 mins. — > acetone, till no 

 more color comes away -^ blot — > dry 



13.12 Gordon 1939 11284,24:405 



reagents required: .1. 10% ammonia; B. ADS 12.2 Gordon 1939; C. water 100, eosin 



Y 0.25, phloxine 0.75; DS D. 11.44 Loffler 1890 

 method: [sections of formaldehyde material]-^/!, 1 min. -^ wash -^ /?, 3 mins.—* 

 wash -* C, 2 mins. -^ wash — > b, 4 mins., 37°C. — >• wash — > abs. ale, till ditTerentiated 

 — > balsam, via xylene 



13.12 JSB stain see DS 11.44 Manwell 1945 (note) 



13.12 Langeron 1934 see DS 11.44 Langeron 1934 



