DS 13.13 DYE STAINS OF GENERAL APPLICATION 349 



13.13 Groat 1936 11281, 21:978 



PREPARATION OF DRY STOCK! Dissolve 1.2 eosin y in 100 water. Dissolve 1 methylene 



blue, 0.2 methyl violet 2 B, 0.04 thionine in 10 water. Mix solutions, heat to 50°C. and 



hold at 37°C., 24 hours. Filter. Wash and dry filtrate. 

 FORMULA OF WORKING SOLUTION: methanol 100, dry stock 0.5 

 method: [blood smears] — > stain, on slide, 5 mins. — > plunge in distilled water till smear 



rosy-pink — ♦ dry 



13.13 Kingsley 1937 11284, 22:524 



stock solutions: I. water 80, phosphate buffer pH 6.920, methylene blue 0.070, methyl- 

 ene azur A 0.025, II. acetone 70, methanol 20, glycerol 10, methylene violet 0.018, 

 eosin Y 0.065 

 WORKING solution: stock I 50, stock II 50 



13.13 Kuhn 1933 19764d, 7 :758 



PREPARATION OF DRY STOCK: Mix 9 ammonia with 6 2% copper sulfate. Add this to 200 

 0.5% meth3dene blue and leave 24 hours at 18°-20°C. Then add, in small amounts and 

 with continuous agitation, 40 2% eosin Y. Filter, wash ppt. with 4% ammonia, and 

 dry. 

 stock solution: methanol 60, glycerol 30, dry stock 0.36 

 w^ORKiXG solution: stock solution 25, methanol 75 



13.13 Lillie 1948 azur-eosin Lillie 1948, 82 



PREPARATION OF DRY STAIN: Dissolve 1 azur A or C in 60 water. Add 0.8 eosin B or Y dis- 

 solved in 10 water. Mix solutions. Filter. Wash and dry ppt. 

 PREPARATION OF STOCK SOLUTION: glycerol 50, methanol 50, dry stock 1 

 PREPARATION OF STOCK BUFFERS: I. Water 75, methanol 25, sodium phosphate, dibasic 



2.84; II. water 75, methanol 25, citric acid 9.12 

 PREPARATION OF WORKING SOLUTION: stock stain 0.5, acetone 5, mixed buffers I and II 2, 



water to make 40 

 method: [sections] —» water —> stain, 1 hr. —> rinse -^ acetone, tiU dehydrated —» 



balsam, via xjdene 

 note: The ratio of buffers I and II in the working stain must be adjusted to suit the 

 fi.xative used and the color balance desired. Lillie, loc. cit., recommends from 0.71: 1.3 

 II to 1:1. This is a slight modification of Lillie 1941 (20540b, 16:1). 



13.13 MacNeal 1922 azur I -methylene violet-eosin Y 11006,78:1122 

 formula: methanol 100, eosin Y 1, azur I 0.6, methylene violet 0.2 

 method: [air-dried smears] — * flood with stain, 2 mins. — > add water, drop by drop, till 



green scum forms on surface -^ leave 1 min. -^ water, thorough wash — » neutral 



mountant, via acetone 

 note: The dry dyes, mixed in the proportion indicated, have appeared in commerce 



under the name MacNeaVs Tetrachrome. 



13.13 Maximow 1909 see DS 12.37 Maximow 1909 



13.13 Maximow 1924 azur-eosin 11250,34:549 



reagents required: A. water 100, DS 11.122 Delafield 1885 0.1; B. 0.1% eosin Y 10, 



water 100, 0.1% azur II 10 

 method: [sections from F 3700.1010 fixed material]—* water -^ A, 24 hrs. -^ water—* 



B, 24 hrs. — > 95% ale, till differentiated -^ balsam, via xylene 



13.13 Medalia, Kahaner, and Singer 1944 see DS 23.33 Medalia et al. 1944 



13.13 McNamara 1933 11284, 18:752 



REAGENTS REQUIRED: A. water 90, ADS 12.2 Lugol (1905) 10; B. 0.5% sodium thio- 



sulfate; C. water 100, DS 13.13 Slider and Downey 1929 10, acetone 10, methyl ale. 10, 



0.5% sodium carbonate 2; D. ADS 22.1 Wolbach 1911 

 method: [4 M sections of F 3700.0010 Zenker 1894 material] — » A, 30 mins. — > 95% ale. 



wash — * water, wash — * B, 10 mins. —^ wash — > C, 15 mins. — » D, till differentiated —* 



balsam, via acetone and xylene 



13.13 Pappenheim 1908 panoptic stain — compl. script. 



13172, 4:1244 

 REAGENTS REQUIRED: A. DS 13.11 May-Grimwald 1902; B. water 97, DS 13.13 Giemsa 

 1902 3 



