350 METHODS AND FORMULAS DS 13.13-DS 13.2 



method: [dry smear] — * A, flooded on slide, 3 mins. -^ add 10 drops distilled water, mix 

 well, leave 1 min. -^ drain -^ B, 10-15 mins. -^ wash off with jet of distiUed water -^ 

 wash, till differentiated — » drain, dry 



note: Agulhon and Chavannes 1919 (6630, 82:149) differentiate with 1% boric acid or 

 1% monosodiura phosphate. Otherwise the technique is identical. Wolbach 1911 

 (11006, 56:345) uses his resin alcohol (ADS 22.1, Wolbach 1911) for differentiation. 

 Langeron 1942, p. 751 refers to his "method for sections" as Wolbach's technique. 



13.13 Pappenheim 1912a 766,42:525 



REAGENTS REQUIRED: A. water 75, DS 13.11 May-Griinwald 1902 25; B. water 95, 



DS 13.13 Giemsa 1902; C. 0.2% acetic acid 

 method: [5 M paraffin sections of F 7000-1000 Orth 1896 or F 3700.1000 Helly 1903 



material, dewaxed and brought to water] — + A, 15 mins., 37°C.^^ B, 40 mins., 37°C. — > 



rinse — > rinse -^ C, till differentiated -^ balsam via acetone 



13.13 Pappenheim 1912b 8545, 13:340 



REAGENTS REQUIRED: A. Water 75, DS 13.11 May-Griinwald 1902; B. water 97, DS 13.11 



Pappenheim 1911 3; C. 0.1% picric acid 

 method: [water] ^ A, 15 mins. at 37°C. ^ B, 30 mins. at 37°C. -^ wash ^ C, till 

 differentiated-^ balsam, via graded acetone-xylene mixtures 



13.13 Shortt 1918 9940, 6:124 



REAGENTS required: A. DS 11.44 Langeron 1908 10, water 90; B. eosin Y 0.01, water 

 100 



method: [methanol-fixed smears] -^ 1 A mixed with 1, 2, or 3 S (proportion established 

 by trial) -^ quick rinse -^ abs. ale, till differentiated -^ water, to stop differentiation 

 — » dry — > balsam 



note: The original calls for a 0.1% solution of BorreVs blue. A above is identical in com- 

 position to such a solution. 



13.13 Slider and Downey 1929 azur-eosin iVIcClung 1929, 246 



formula: glycerol 50, azur II 0.16, azur Il-eosin 0.6, methanol 50 

 method: [smears, treated with methanol or DS 13.11 Jenner 1899 or DS 13.11 May- 

 Griinwald 1902] -^ equal parts stain and water (or pH 6.4 buffer), 15 mins. — > water, 

 till differentiated — + blot -^ dry 

 note: Gatenby and Cowdry 1928, 490 specify 75 methanol to 25 glycerol. 



13.13 Svihla 1924 11006,83:2093 



preparation of dry stock: To 50 water add 0.69 methj'lene blue, 0.44 silver oxide, 0.38 



sodium bicarbonate. Boil 1 hour and decant supernant liquid which is added to 50 



0.62% eosin. Mix thoroughly, filter, wash, and dry ppt. 

 WORKING solittion: methanol 100, sodium phosphate, dibasic 0.125, potassium di- 



hydrogen phosphate 0.080, potassium hydroxide 0.004 



13.13 Ugruimov 1928 see DS 21.3 Ugruimov 1928 



13.13 Villain and Comte 1,933 see DS 23.3 Villain and Comte 1933 



13.13 Wolbach see DS 13.13 Langeron 1942b (note) 



13.13 Wolbach 1911 see DS 13.13 Pappenheim 1908 



13.13 Wolbach 1919 see DS 23.12 Wolbach 1919 



13.2 Techniques Employing the eosinates themselves. The first and still 



Thiazins and Their Eosinates the best known formula is that of Mann 



IN Combination with Other 1894, though in French literature it has 



Dyes largely been replaced by the method of 



The combination of the thiazin-eosin- Dominici 1902. The preoccupation of the 



ates with orange G (DS 13.21) is almost French school with the work of Dominici 



as old as the utilization of the thiazin- has led to the production of such almost 



