356 METHODS AND FORMULAS DS 13.31-DS 13.32 



13.31 Walton 1939 see DS 23.219 Walton 1939 



13.32 METHYL GREEN IN COMBINATION WITH OTHER DYES 



13.32 Auerbach test. 1930 Guyer acid fuchsin-methyl green 



Guyer 1930, 230 

 STOCK solutions: I. 0.1% acid fuchsin; II. 0.1% methyl green 

 WORKING solution: stock I 40, acetic acid 0.005, stock II 60 



method: [3 X sections of mercuric fixed material] -^ water — * stain, 15 mins. — > 95% ale, 

 till green clouds cease — ♦ abs. ale. rinse — > balsam, via xylene 



13.32 Biondi test. 1888 Heidenhain orange G-acid fuchsin-methyl green 



16155, 63 (suppl.): 40 



stock solution: mix 100 sat. sol. (circ. 11%) orange G with 20 sat. sol. {circ. 13%) acid 

 fuchsin. Add slowly and with constant agitation 50 sat. sol. {circ. 5%) methyl green 



WORKING formula: Water 100, stock 1-2 



method: [sections] -^ water —>■ stain, 6-24 hrs. -^ balsam, via usual reagents 



notes: This stain is variously attributed to Ehrlich, Ehrlich-Biondi, and Ehrlich- 

 Biondi-Heidenhain. Heidenhain (loc. cit.) explains quite clearly " — welche dieselben 

 Ingredientien enthdlt, ivie die von Babes empfohlene Ehrlich'sche Mischung {Berhend's 

 Ztschr f. Mikroskopie, Bd IV, S 232) aber in anderen Verhdltnissen." This would seem 

 to dispose of the "Ehrlich" in the name of the mixture. These new proportions, how- 

 ever, were stated {loc. cit.) as "nach Versuchen von Biondi" and the mixture was later 

 referred to (p. 43) as Biondi'sche Flussigkeit. 



13.32 Cooper 1931 see DS 22.11 Cooper 1931 



13.32 Ehrlich 1888 Ehrlich's Triacid—compl. script, see DS 13.32 Biondi (1888) (note) 



13.32 Ehrlich 1898 test. Lee 1905 orange G-acid fuchsin-methyl green 



Lee 1905, 212 

 preparation: Mix 16 sat. sol. {circ. 11%) orange G with 7.5 sat. sol. (circ. 13%) acid 

 fuchsin. Dilute mixture with 40 50% ale. Add slowly and with constant agitation 15 

 sat. sol. {circ. 5%) methyl green. Then add 10 each 95% ale. and glycerol. 



13.32 Foley 1930 rnethyl green-acid fuchsin 763, 45 :340 



REAGENTS REQUIRED: A. 2% methyl green 80, 0.1% acid fuchsin 20; B. 0.1% hydro- 

 chloric aeid 



method: [sections of osmic fixed, or mordanted, material] -^ running water, overnight -^ 

 A, 24 hrs. -^ blot — > B, till differentiated — » balsam, via carbol-xylene 



result: chromatin, green; muscle, brick red; other tissues, pink. 



13.32 Foley 1931 methyl green-acid fuchsin-orange G 763,49:15 



preparation: To 10 glycerol and 20 0.1% acid fuchsin and 30 0.1% orange G, add 



drop by drop, with constant agitation, 30 0.25% methyl green. 

 method: [water] — > stain, 12-24 hrs. -^ blot — * 95% ale, till differentiated, 5-30 sees. — > 



balsam, via carbol-xylene 

 note: a detailed description of the use of this stain is given under 13.30 above. 



13.32 Grosso 1914 see DS 12.211 Grosso 1914 



13.32 Guinard 1889 methyl green-acid fuchsin 19076,1:19 



preparation: Mix 1% methyl green with 1% acid fuchsin in such proportion as give a 

 violet solution. Add acetic aeid to pH 3. 



13.32 Heidenhain 1888 see DS 13.32 Biondi (1888) (note) 



13.32 Kahlden and Laurent 1896 see DS 21.3 Kahlden and Laurent 1896 



13.32 Kardos 1911 see DS 21.3 Kardos 1911 



13.32 Krause 1893 mttliyl green-acid fuchsin-orange G 1780,42:59 



formula: sat. sol. {circ. 13%) aeid fuchsin 0.4, sat. sol. {circ. 11%) orange G 0.7, sat. 

 sol. {circ. 5%) methyl green 0.8, water 100 



