DS 13.32-DS 13.40 DYE STAINS OF GENERAL APPLICATION 3o7 



13.32 Krause 1911 test. 1948 Romeis methyl green-acid fuchsin-orange G 



Romeis 1948, 1G9 

 formula: water 100, methyl green 3.4, acid fuchsin 4.2, orange G 3 

 preparation: Grind the (hy dyes together to a line powder. Dissolve in water. 

 method: As Biondi (1888) 



13.32 Maresch 1905 see DS 13.43 Maresch 1905 



13.32 Mayer 1901 test. 1901 Lee and Mayer methyl green-acid fuchsin-orange G 



Lee and Mayer 1901, 197 

 formula: water 60, glycerol 15, 95% ale. 25, orange G 2.6, acid fuchsin 4, methyl green 



1.3 . . . • 



preparation: Grind dyes together and dissolve in mixed solvents. 



13.32 Moore 1882 see DS 21.3 Moore 1882 



13.32 Morel and Doleris 1902 6630, 54:1255 



formula: DS 13.32 Ehrlich 1898 50, 8% formaldehyde 50, acetic acid 0.1 



13.32 Oppell test. 1895 Rawitz methyl green-acid fuchsin-picric acid 



Rawitz 1895, 71 

 REAGENTS REQUIRED: A. 1% methyl green 60, 1% eosin 1, 1% acid fuchsin 20, abs. ale. 



20; B. sat. sol. picric acid 80, abs. ale. 20 

 method: [sections] — > A, 15 mins. — > B, 30 sees. — > abs. ale, minimum possible time -^ 

 balsam, via usual reagents 



13.32 Squire 1892 methyl green-acid fuchsin Squire 1892, 37 



preparation: Mix 30 0.5% methyl green with 10 1.5% acid fuchsin. 



13.32 Stropeni 1912 methyl green-acridine red 23632, 29 :3()2 



reagents required: A. water 100, sodium borate 1; B. water 100, glycerol 20, methanol 



30, phenol 2, methyl green 0.05, acridine red 0.25 

 preparation of B: Grind each dye with 1 phenol and wash out each mortar with 50 



water. Mix washings and add other ingredients. 

 method: water -^ A, 10 mins. — > rinse — » B, 30 mins. — > abs. ale. till differentiated —* 



balsam via xylene 



13.32 Thome 1898 methxjl green-acid fuchsin-orange G 1780, 52 :820 



formula: sat. sol. {circ. 13%) acid fuchsin 0.15, sat. sol. (circ. 11%) orange G 0.35, sat. 

 sol. (circ. 5%) methyl green 0.6, water 100 



13.4 Techniques Employing Acid Fuchsin as the Nucleak Stain 



Within this subdivision of the complex 20540b, 11:101) prefers phosphotungstic 



staining techniques he the majority of acid. No one formula in either of these two 



the methods which are understood today groups can be singled out as better than 



whenever the term triple stain is used, another, and only one of them (Heiden- 



Originated by Mallory in 1901, they de- hain 1905) has become sufficiently well 



pend for the most part upon the fact that known to acquire a popular name. This 



phosphomolybdic acid will extract acid stain is frequently referred to as Heiden- 



fuchsin from collagens and leave it in hain's azan because azocarmine is used as 



muscle and nuclei. Various mixtures are the first solution, 

 then used differentially to stain the decol- 

 orized tissues. The original method of 13.40 typical examples 



Mallory used methyl blue and orange G 



and has been widely copied. So numerous p^p^j, nation of a transverse section of 



have these formulas become that it is Treparation ot a transverse section oi 



.., ■• , „+„i;;j« Amphioxus using the acid fuchsm- 



necessary in the present instance to divide •,• i , r^ ^ ■ <• 



,, • 1 xu • I, V, 1 u.i;^ anihn blue-orange G stain oi 



them into those using phosphomolybdic ° 



acid (DS 13.41), and those using phospho- Maiiory lyui 



tungstic acid (DS 13.42), though in point Amphioxus is a difficult subject from 



of fact the results of the stains can scarcely wliich to prepare satisfactory sections, the 



be distinguished. Mallory himself (1936; more so as it is almost impossible now- 



