360 METHODS AND FORMULAS DS 13.41-DS 13.42 



13.41 Kricheski 1931 10540b, 6 :97 



REAGENTS REQUIRED: A. 0.25% acid fuchsin; B. 2% methyl blue 30, 1% orange G 30, 



1% phosphomolybdic acid 30 

 METHOD : water — * A, 1-3 mins. -^ water, thorough rinse —> B, 3-5 mins. — > water, quick 



rinse -^ 70% ale, 2 or 3 dips -^^ 95% ale, 2 or 3 dips -^ abs. ale. till differentiated 1-3 



mins. -^ balsam, via xylene 

 result: as Mallory 1901. 



13.41 Ladewig 1938 see DS 13.6 Ladewig 1938 



13.41 Lee-Brown 1929 see DS 13.41 Mallory 1901 (note) 



13.41 Lewis and Miller 1938 see DS 21.421 Lewis and Miller 1938 



13.41 Mallory 1901 11189,5:15 



REAGENTS REQUIRED: A. 1 % acid fuchsin; B. 1% phosphomolybdic acid; C. water 100, 

 methyl blue 0.5, orange G 2, oxalic acid 2 



method: [water] — > .4, 2 mins. — * water, thorough rinse — > B, 2 mins. — > water, quick 

 rinse -^ C, 15 mins. -^ water, thorough wash — > abs. ale, till differentiated -^ balsam, 

 via xylene 



results: nuclei, red; coUagens, blue; nerves and glands, violet; muscle, red; erythro- 

 cytes and keratin, orange. 



note: Mallory 1936 (20540b, 11:101) substituted 1% phosphotungstic acid in B. A 

 detailed description of the application of this stain is given under DS 13.40 above. 

 Lee-Brown 1929 (11597, 21:259) differs only in using sol. C before sol. B. Rexed 

 and Wohlfart 1939 (23632, 56:212) recommend that A above be buffered to pH 3.3 

 with citrate. 



13.41 Masson 1912 4956, 14:291 



reagents required: A. 4% ferric alum at 50°C.; B. 1% hematoxylin at 50°C.; C. 2% 

 ferric alum; D. 0.1% acid fuchsin; E. 1% phosphomolybdic acid; i^. 1% anilin blue 

 50, 1 % phosphomolybdic acid 50 

 method: [water] —> A, 5 mins., 50°C. -^ water, 50°C. rapid rinse-* 5, 10-15 mins., 

 50°C. — ^ C, till nuclei alone colored — > running water, 15 mins. — > D, 10 mins. -* tap 

 water, if overstaining has occurred — * E, 5-10 mins. — > F, 20 mins. to 1 hr. — » water, 

 quick rinse — > 95% ale, quick rinse — > abs. ale till dehydrated — > balsam, via xylene 



13.41 Maxwell 1938 see DS 21.421 Maxwell 1938 



13.41 Milligan 1946 Tech. Bull, 7 :57 



reagents required: A. water 75, 95% ale 25, potassium dichromate 2.25, hydrochloric 



acid 2.5; B. 0.1% acid fuchsin; C. 1% phosphomolybdic acid; D.2% orange G in 1% 



phosphomolybdic acid; E. 1; acetic acid; F. 0.1% either fast green FCF or anilin blue 



in 0.2% acetic acid 

 method: [sections of formaldehyde material] — * water — * A, 5 mins. rinse —* B, 5 mins. 



— > rinse — > C, 1-5 mins. — > D, 5-10 mins. —^ rinse —^E,2 mins. — » F, 5-10 mins. — > E, 



3 mins. — > 95% ale. — ♦ balsam, via usual reagents 



13.41 Rexed and Wohlfart 1939 see DS 13.41 Mallory 1901 (note) 



13.41 Schneidau 1937 21400a, 56:260 



reagents required: A. 1% acid fuchsin; B. 10% phosphomolybdic acid; C. 0.1% 



thionine 

 method: [whole objects] — > A, 2-4 mins. — > wash —* B, 1 min. — » rinse —* C, 2-4 mins. 



—> wash — » balsam, via usual reagents. 

 recommended for: double stained wholemounts. 



13.42 METHODS EMPLOYING THE ACID FUCHSIN-PHOSPHOTUNGSTIC REACTION 



13.42 Cason 1950 20540b, 25 :225 



formula: water 100, phosphotungstic acid 0.5, orange G 1, anilin blue 0.5, acid fuchsin 



1.5 

 method: [Gju sections] —> water -^ stain, 5 mins. —> water, rinse ^ balsam, via usual 



reagents 



