DS 13.42-DS 13.43 DYE STAINS OF GENERAL APPLICATION 3G1 



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13.42 Heidenhain 1905 Heidenhain's Azan — compl. script. 



23G32, 22 :33'J 

 REAGENTS REQUIRED: A. water 100, azocarmine 2, acetic acid 1; B. 0.1% aniline in 95% 

 ale; C. 0.1% hydrochloric acid in abs. ale; D. 5% phosphomolybdic acid; E. water 

 100, orange G 2, anilin blue 0.5, acetic acid 7.5 

 method: water— » A, 1 hr., 60°C. -^ water, quick rinse -^ B, till nuclei well marked 

 dip in C, before examining — » C, thorough rinse — > jD, 2 hrs. — > E, 2-3 hrs. — > water, 

 rinse — >• balsam, via usual reagents 

 result: nuclei, scarlet; muscle, orange; coUagens, blue. 



13.42 Kohashi 1937 . 8542a, 15:175 



reagents required: A. water 100, azocarmine 0.1, acetic acid 1; B. 90% ale. 100, 

 aniline 0.1; C. 1% acetic acid in 95% ale; D. 5% phosphotungstic acid; E. DS 12.31 

 Pasini (1928) (sol. B) 

 method: [sections] —* water — » A, 12-15 mins. 60°C. — > wash -^ B, till nuclei differenti- 

 ated — » C, li-1 mins. — > rinse — >• D, l-i-l hr. -^ rinse -^ E, 15-20 mins. —+95% ale. 

 till differentiated — > balsam, via c^.rbol-xylene 



13.42 Walter 1930 23632, 46 :458 



REAGENTS REQUIRED: A. 2.5% ferric alum; B. 2% phosphotungstic acid; C. DS 12.31 



Pasini (1928) sol. B 

 method: water -^ A, 2-4 hrs. — > water, quick rinse — » B, 10 mins. — * water, quick rinse 



— » C, 15-20 mins. — » 95% ale, till color clouds cease — > abs. ale, 1 min. — > balsam, 



via usual reagents 

 result: nuclei and elastic fibers, purple; coUagens, blue; erythrocytes, orange. 



13.43 METHODS USING NEITHER PHOSPHOTUNGSTIC NOR PHOSPHOMOLYBDIC ACID 



13.43 Auerbach see DS 13.32 Auerbach (1930) 



13.43 Bohm and Oppel 1907 Bohm and Oppel 1907, 115 



stock formulas: I. water 100, orange G 0.05, acid fuchsin 0.05, acetic acid 1, 40% 



formaldehyde 1; II. water 90, methanol 10, 0.25% brilliant cresyl blue 0.25, 40%, 



formaldehyde 1 

 WORKING solution: stock I 50, stock II 50 

 method: water —> stain, 20-30 mins. -^ abs. ale, till differentiated —> balsam, via 



xylene 

 result: nuclei, red-purple; cartilage, blue; bone, orange; muscle, red. 



13.43 Buchholz 1931 see DS 21.41 Buchholz 1931 



13.43 Delamare 1905 see DS 13.7 Delamare 1905 



13.43 Maresch 1905 23681,16:41 



REAGENTS REQUIRED: A. sat. methanol sol. methyl green 50, sat. methanol sol. picric acid 



50; B. Q.lb7o acid fuchsin 

 method: [sections] -* abs. ale -^ A, 10 mins. — > water, rinse -» B, 5-10 sees. -» blot -» 

 — > abs. ale dropped on slide, till color changes from dark violet to blue gray -^ bal- 

 sam, via xylene 



13.43 Roskin 1946 Roskin 1946, 154 



REAGENTS REQUIRED: A. 0.1% indigocarmine in sat. sol. picric acid; B. sat. aq. sol. 



magenta 

 method: [sections] -> A, 10-30 mins. -^ B, poured on slide, left till greenish scum ap- 

 pears — » abs. ale, till difJerentiated 



13.43 Waterman 1937 20540b, 12:21 



PREPARATION OF STOCK SOLUTION! water 100, phcnol 5, magenta 3 

 REAGENTS REQUIRED: A. Water 100, acetic acid 0.6, 40% formaldehyde 0.6, stock 10; 



B. water 100, picric acid 1, acetic acid 2, indigocarmine 0.25 

 method: [sections of material fixed in F 4000.0040 Watermann 1937] -^ water -^ A, 

 5 mins. -^ rinse, 2 sees. -^ B, 90 sees. -+ rinse, 2 sees. -^50% dioxane, 2 sees. -^ bal- 

 sam, via dioxane and xylene 

 result: nuclei, red; collagen, blue; other tissues, yellow and green 



