DS 13.51 DS 13.6 DYE STAINS OF GENERAL APPLICATION 3G5 



REAGENTS REQUIRED: A. stock I 50, stock IT 15, stofk TIT 15; B. 1% safranin; C. 0.5% 



potassium dichroinate 

 method: [sections] — > watei- —* A, 10 mins. —^ wash -^ B, 10 mins. — » thorough wash — ♦ 



C, 10-30 mins. — > wash — > balsam, via usual reagents 

 result: nuclei, black with red granules; protoplasm violet; collagen, blue; elastic fibers, 



red 

 recommended for: skin. 



13.51 Unna test. 1928 Schmorl safranin-anilin blue Schmorl 1928, 176 



reagents required: ^1. 1% safranin; B. water 100, tannin 15, anilin blue 0.5 

 method: [sections] ^ a, 10 mins. —^ thorough wash — > B, 10-15 mins. —> thorough 

 wash — ^ abs. ale. minimum possible time -^ balsam, via xylene 



13.6 Techniques Employing Hematoxylin as the Nuclear Stain 



Hematoxylin is, of course, the commonest nuclear stain to be employed before any of the 

 plasma techniques (DS 12). The formulas here given are those in which the nuclear staining 

 is an integral portion of a complex technique which cannot be employed in combination with 

 any other nuclear stain. 



13.6 Barbrow 1937 11284, 22:1175 



STOCK solutions: I. 1% hematoxylin in 95% ale. (well "ripened"); II. water 99, ferric 



chloride 2, hydrochloric acid 1; III. water 100, picric acid 1, acid fuchsin 1 

 working solution: stock I 25; stock II 10; stock III 50 



method: [frozen sections of unfixed tissues] -^ stain, 1 min. —» wash ^ balsam, via 

 usual reagents. 



13.6 Brown and Brenn 1931 see DS 23.221 Brown and Brenn 1931 



13.6 Delamare 1905 test. 1907 Bohm and Oppel Bohm and Oppel 1907, 131 



stock solutions: I. abs. ale. 100, hydrochloric acid 2, orcein 2; II. sat. aq. sol. picric 



acid 100, sat. aq. sol. acid fuchsin 0.5, DS 11.123 Ehrlich 1886 1 

 reagents required: A. stock I 50, stock II 50; B. 0.1% hydrochloric acid 

 method: [sections] — * water —> A, 30 mins. -^ B, rinse -^ tap water, to "blue" hema- 

 toxylin -^ balsam, via usual reagents 

 result: nuclei, violet; muscle and general cytoplasm, yellow; collagens, red; elastic 

 fibers, black. 



13.6 Friedlander 1889 Friedlander 1889, 94 



formula: abs. ale. 30, hematoxylin 0.5, glycerol 30, sat. sol. potassium alum 30, 1% 



eosin 10 

 preparation: Dissolve the hematoxylin in the ale. Add the glycerol and alum. Leave 

 1 week. Filter. Add the eosin sol. to filtrate. 



13.6 Galiano 1928 test. 1928 Findlay 11360, 48:314 



reagents required: A. 3% ferric alum; B. water 80, acetic acid 20, hematoxylin 0.2; 



C. 95% ale. 75 m acetic acid 25, eosin 1.5; D. 0.1% ammonia in 95% ale. 

 method: [sections] —> water -^ a, 15 mins. — > 5, till nuclei darkly stained —>• wash, 



15 mins. -^70% ale, 1 min. -^ C, till differentiated — » D, wash— > balsam, via usual 



reagents 



13.6 Kefalas 1926 11360, 46:277 



reagents required: A. DS 11.121 Kefalas 1926; B. sat. sol. Biebrich scarlet in acetone 

 method: [sections] —> acetone -^ a, till slightly overstained — > B, till counterstained 

 and differentiated —> acetone —y balsam, via xylene 



13.6 Koneff 1936 763, 66:173 



reagents required: A. 5% ferric alum; B. DS 11.122 Harris 1900; C. water 100, anilin 

 blue 0.03, phosphomolybdic acid 5, oxalic acid 0.6 



13.6 Ladewig 1938 23032,55:215 



reagents required: A. DS 11.121 Weigert 1903; B. 1% phosphotungstic acid; C. water 



100, methyl blue 0.5, orange G 2, oxalic acid 2, acid fuschin 1 

 method: [sections of formaldehyde material] -^ water —» .1, 3-5 mins. rinse —> B, 2 



mins. — > rinse ^ C, 4 mins. -^ quick rinse — > 95% ale, till dehydrated — > balsam, via 



xylene 



