3GG METHODS AND FORMULAS DS 13.6 



13.6 Lillie 1945 4349, 25 :33 



REAGENTS required: A. sat. 95% ale. sol. picric acid; B. DS 11.121 Weigort 1903; C 1% 



Biebrich scarlet in 1% acetic acid; D. 3% ferric chloride; E. water 99, acetic acid 1 



and either anilin blue 1 or methyl blue 1 or wool green S 1 ; F. 1 % acetic acid 

 method: [sections] — > 95% ale. — > A, 2 mins. -^ thorough wash -> B, 6 mins. — > wash 



-^ C, 4 mins. — > D, 2 mins. -^ £^, 3-5 mins. -^ rinse -^ F, 2 mins. — > balsam, via 



acetone and xylene 

 result: nuclei, brown black; muscle and cytoplasm, red; connective tissue, blue or 



green. 



13.6 mile 1948 Lillie 1948, 149 



REAGENTS REQUIRED: A. DS 11.121 Weigert 1904; B. 0.02% fast green FCF; C. 1% 



acetic acid; D. 0.1% Bismarck brown Y in 1% acetic acid 

 method: [sections] -^ water -^ A, Q mins. -^ wash — ♦ 5, 3 mins. — >• C, wash — >■ D, 4-6 



mins. -^ balsam, via usual reagents 

 result: nuclei, black; general cytoplasm, gray green; mucus, cartilage, cell granules, 



brown. 

 note: Magenta, or new magenta, may be substituted for Bismarck brown in D. Eosin Y 



may be substituted in B, in which case crystal violet or malachite green should be 



substituted in D. 



13.6 Lowenthal 1892 test. 1907 Bohm and Oppel Bohm and Oppel 1907, 119 



preparation of stock solutions: I. Dissolve 0.4 carmine in 100 0.05% sodium hydrox- 

 ide. Add 0.25 picric acid; II. to 0.5 hematoxylin dissolved in 50 abs. ale. add 50 1: 

 ammonium alum. 



WORKING solution: stock I 100, stock II 20, acetic acid 2.5 



PREPARATION OF WORKING SOLUTION: Mix the stock solutions slowly and with constant 

 agitation. Leave 24 hours. Filter. Ripen 4 weeks. 



method: [sections] -^ water — > stain, 24 hrs. -^ wash -^ balsam, via usual reagents 



13.6 Mollier 1938 23632,55:472 



REAGENTS REQUIRED: A. DS 21.12 Unua-Tanzcr (1896); B. DS 11.121 Weigert 1904; 

 C 1% hydrochloric acid in 70% ale; D. water 100, azocarmine 2, acetic acid 1; E. 

 5% phosphotungstic acid; F. water 100, naphthol green B 1, acetic acid 1 

 method: [sections] —> 70% ale. -^ A, 12 hrs. — > wash, till no more color comes away 

 — > B, 1-3 mins. — > rinse -^ C, till nuclei well differentiated — > wash, 15 mins. -^ D, 

 15-30 mins. —^ rinse -^ E, 3 changes, 2-6 hrs., till collagen decolorized — * rinse -^ F, 

 15-30 mins. — > 95% ale., 30 sees, with constant agitation -+ balsam, via usual reagents 

 result: nuclei, deep blue; general cytoplasm, purple; elastic fibers, black; collagen, 

 green; erythrocytes, scarlet. 



13.6 Mollendorf test. 1946 Roskin Roskin 1946, 158 



reagents required: A. DS 11.121 Hansen 1905; B. 1% eosin in 0.3%, acetic acid; C. 2% 



phosphomolybdic acid; D. 1% methyl blue 

 method: [sections] —> water —> a, 5 mins. ^ distilled water, rinse ^ running water, 



wash -^ B, 20 mins. —>■ rinse -^ C, 10 sees. -^ rinse -^ D, 1-2 mins. -^ rinse -^95% 



ale. till color clouds cease — > abs. ale. balsam, via xylene 



13.6 Paquin and Goddard 1947 4349, 27:198 



REAGENTS REQUIRED: A. DS 11.121 Paquiu and Goddard 1947; B. 0.5% picric acid in 



95% ale; C. water 100, phosphotungstic acid 0.1; eosin 0.07, phloxine 0.03, orange G 



0.1; D. 0.2% phosphotungstic acid; E. 0.4% acetic acid; F. 0.04% anihn blue in 1% 



acetic acid 

 method: [sections of F 5600.1000 Masson (1947) material]-^ water-* A, 5 mins. -► 



wash, 5 mins. -^ B, 15-20 sees. -> wash ->■ C, 5 mins. -> D, 5 mins. -^ E, double 



rinse -> F, 5 mins. -^ E, double rinse -^ D,b mins. -^ E, 30 mins. -^ 95% ale, 3 dips 



-^ balsam, via isoamyl ale. and toluene 

 result: nuclei, black; elastic tissue, cherry red; other connective tissues, blue; general 



cytoplasm, pink. 



13.6 Papanicolaou 1941 see DS 23.4 Papanicolaou 1941 



