DS 13.6-DS 13.7 DYE STAINS OF GENERAL APPLICATION 367 



13.6 Petragnani 1928 see DS 23.13 Petragnani 1928 



13.6 Renaut k^t. 1889 Friedlander Friedlander 1889, 94 



formula: sat. sol. potassium alum in glycerol 65, sat. sol. eosin Y 15, sat. ale. sol. hema- 

 toxylin 20 



13.6 Reeve 1948 205401), 23:1:5 



REAGENTS REQUIRED: A. watcr 90, DS 11.122 Dolaficid (1885) 10; li. water 40, 95% ale. 



60, safranin 0.01, sodium acetate 0.01; ('. xylene 75, abs. ale. 25, sat. sol. fast green 



FCF in 50:50 clove oil— abs. ale. 2-5 

 method: [sections] —^ water — * A, 5-15 mins. —' wash -^ B, 5-15 mins. — * rinse — » 95% 



ale., till no more color comes away -^ C, 1-3 mins. -^ balsam, via xjdene 

 recommended for: general plant histology. 



13.6 Reynolds 1936 see DS 23.34 Reynolds 1936 



13.6 Romeis 1948 Romeis 1948, 364 



REAGENTS REQUIRED: A. DS 21.13 Wcigert 1898 (working sol.); B. DS 11.121 Weigert 



1903; C. water 100, azophloxine 0.5, acetic acid 0.2; D. 1% acetic acid; E. water 100, 



phosphomolybdic acid 4, orange G 2; F. water 100, light green 0.2, acetic acid 0.2 

 method: [sections] — * 80% ale. —>■ A, 15 mins. -^ wash — » B, 2-3 mins. — > thorough 



wash — ^ C, 5 mins. — > D, wash — > E, till collagen decolorized -^ D, rinse -^ F, 5 mins. 



-^ D, 5 mins. — » abs. ale, least possible time -^ balsam, via xylene 

 result: nuclei, black; muscles and general cytoplasm, red; collagen, green; elastic 



fibers, black. 

 note: The C, D, E, F solutions are from DS 12.31 Goldncr 1938. 



13.6 Slater and Dornfeld 1939 20540b, 14:103 



REAGENTS REQUIRED: A. DS 11.123 Harris 1900; B. 1% safranin () in sat. aq. sol. aniline; 



C. 0.5% fast green in 95%, ale. 

 method: [sections by dioxane technique of amphibian embryos from F 5000.1010 



Puckett 1937] -^ A, 5 mins. — » C, 2-5 mins., till yolk granules red on green cytoplasm 



—>■ balsam, via usual reagents 



13.7 Other Complex Techniques of General Application 



This is the final division of complex clear stain and picric-indigo-carmine as 

 staining techniques in which it has been the contrast. This gives magnificent pic- 

 necessary to place all those miscellaneous tures on embryonic material and is so easy 

 techniques which do not fall into the pre- to use that it is safe even in the hands of 

 ceding classifications. Few of them are well beginning classes. The mixture of Tw-ort, 

 known, and the three techniques of Becher of neutral red and liglit green, was at one 

 1921 should receive wider attention than time even more popular for staining blood 

 they have received. People have become films than the conventional methylene 

 accustomed to regarding the soluble lakes blue-eosonates; it is now rarely heard of, 

 of the oxazines, when they are used at all, and then only for staining of blood para- 

 as nuclear stains, hence no attention ap- sites. It would be an excellent general- 

 pears to have been given to those other purpose tissue stain if it were not for the 

 formulas published by Becher in which extreme sensitivity of both of its ingrcdi- 

 these dyes are used with varying mordants ents to variations in the pll of the final 

 for the purpose of providing a single-solu- mounting media. The methods of Lonn- 

 tion polychrome stain. These stains can berg 1891 and Lynch 1930 are of interest 

 quite safely be used for research purposes, only in that they provide methods of 

 since their results are reproducible and double-staining wholemounts. There is no 

 they are stable to fight and acid. Attention justification for this from a research point 

 should also be drawn to the formula of of view, but they make the most amusing 

 Shumway 1926, which is a rather unusual mounts, which can be used for popular dis- 

 triple stain involving magenta as the nu- play very cfi"ectively. 



