368 METHODS AND FORMULAS DS 13.7 



13.7 Alzheimer 1910 see DS 13.7 Mann 1894a (note) 



13.7 Becher 1921a polychrome gallamin blue Becher 1921, 70 



formula: water 100, sodium alum 5, gallamin blue 0.5 

 preparation: BoU 5 minutes. Cool. Filter. 

 method: [sections] —> water -^ stain, 24 hrs. —> water, wash —» balsam, via usual 



reagents 

 result: nuclei, black; cartilage, violet. 



13.7 Becher 1921b polychrome quinalizarine Becher 1921, 55 



formula: water 100, chrome alum 5, quinalizarin 0.5 

 preparation: Boil 5 minutes. Cool. Filter. 

 result: nuclei, blue; muscle and nerves, red. 



13.7 Becher 1921c polychrome coeleslin blue Becher 1921, 73 



formula: water 100, chrome alum 5, coelestin blue 0.5 

 preparation: Boil 5 minutes. Cool. Filter. 



method: [sections] -^ water — > stain, 24 hrs. -^ water, wash —* balsam, via usual reagents 

 result: blue black nuclei, with a violet to red polychrome staining of connective tissues. 

 For coelestin blue as a nuclear stain, see DS 11.41. 



13.7 Bensley and Bensley 1938 acid fuchsin-crystal violet 



Bensley and Bensley 1938, 97 

 preparation of dry stain: Add a sat. sol. acid fuchsin to a sat. sol. crystal violet till 



no further ppt. produced. Filter. Wash and drj- ppt. 

 preparation of stock solution: abs. ale. 100, diy stain from above to sat. 

 reagents required: .1. water 72, abs. ale. 18, stock solution 10; B. clove oil 75, abs. 



ale. 25 

 method: [sections] -^ water -^.4,5 mins. — > blot — * acetone, till dehydrated —* benzene 

 — » B, till differentiated -^ benzene —> balsam 



13.7 Bohm and Oppel 1907 Bismarck brown-dahlia violet-methyl green 



Bohm and Oppel 1907, 127 

 REAGENTS REQUIRED: A. Sat. sol. {circ. 1.5%) Bismarck brown: B. 12.212 Roux 1894 

 method: water — > A, 10 mins. — > water, quick rinse -^ B, 1 min. — > abs. ale. till differ- 

 entiated, few sees. -^ carbol-xylene — * balsam, via xylene 

 result: as 12.212 Roux 1894 but with black nuclei and brown cartilage. 



13.7 Bonney 1908 methyl violet-orange G-pyronin 22575,193:547 



REAGENTS required: A. Water 100, methyl violet 0.25, pyronin 1; B. acetone 100 2% 



orange G q.s. 

 PREPARATION Of B: To 100 acetone add 2% orange G till ppt. first formed just redis- 



solves. 

 method: [sections, mercuric-fixed, or mordanted]—* water —> A, 2 mins. — > B, flooded 



on drained slide, 1 min. — > balsam, via acetone and xylene 

 result: nuclei, purple; plasma, red and yellow. 



13.7 Borrel 1901 see DS 23.33 Borrel 1901 



13.7 Buzaglo 1934 quinalizar in-acid alizarin blue-alizarin viridine 



4285a, 11 :40 



reagents required: A. DS 11.41 Becher 1921b; B. 70% ale. 100, hydrochloric acid 1, 

 orcein 1; C. water 100, aluminum sulfate 10, acid alizarin blue 5; D. 5% phospho- 

 molybdic acid; E. water 100, hydrochloric acid q.s. to make pH 5.8, alizarin viridine 

 0.2 



PREPARATION OF C: Boil 10 minutes. Cool. FUter. 



method: [sections] —> water -^ A, 2i hrs. -^ rinse -^ B, S changes, 5 mins. in each — » 

 rinse — * C, 7 mins. — » rinse — > D, till muscles differentiated —* wash -^ E, 7 mins. — > 

 blot -^ abs. ale. minimum possible time — » balsam, via carbol-xylene and xylene 



result: nuclei, dark blue; elastic fibers, brown; muscle, blue; cartilage, green. 



