DS 13.7 DYE STAINS OF GENERAL APPLICATION 3C9 



13.7 Calleja 1897 airminr-picro-indiyocannine 23632, 15:323 



REAGENTS UKyuiHKD: .1. sat. sol. Uthiuiii carl)onatc 100, canniiic 2; li. {).[% liydro- 



chloric acid; C. sat. sol. picric acid 100, indigocarmine 0.25; D. 0.2% acetic acid 

 method: [sections] —>■ A, 5-10 mins. — > B. 20-30 sees. -^ wash—* C, 5-10 mins. — » D, 

 few sees. — > balsam, via usual reagents 



13.7 Canon 1937 14900,139:549 



formula: 70% ale. 100, chlorazol black E 1 

 method: [sections, plant or animal tissue] — > stain, 15-30 mins. — > wash — > balsam, via 



usual reagents 

 result: a very pleasing, and well-differentiated series of gray tones with some green. 

 note: This stain is often attributed to Darrow 1940 (20540b, 15:()7) who introduced it 



into American literature. 



13.7 Castroviejo 1932 magenta-picro-indigo carmine 5i)lb, 2:135 



reagents required: A. water 100, 40% formaldehyde 0.6, DS 11.43 Ziehl 1882 5; B. 



DS 12.221 Cajal 1895 100, acetic acid 0.6 

 method: [sections of formaldehyde material]^ waters A, till nuclei well stained^ 



wash — > B, till stained -^ wash — » balsam, via usual reagents 



13.7 Darrow 1940 see DS 13.4 Canon 1937 



13.7 Dobell 1919 methyl bluc-eosin Y-orange G Dobell 1919, 7 



REAGENTS REQUIRED: A. DS 13.5 Mann 1894; B. 70% ale. 90, sat. sol (circ. 0.2%) orange 



G in abs. ale. 10 

 method: [smears]-^ waters .4, 12 hrs. — > B, applied from drop bottle, till diiTerenti- 



ated — > abs. ale, minimum possible time — > balsam, via xylene 



13.7 Drew-Murray 1919 picro-acid fuchsin-nile blue 1200, 6 :77 



REAGENTS REQUIRED: A. DS 12.221 van Gieson 1896; B. 2% nile blue sulfate 

 method: [water] —> a, 1-3 mins. ^ water, thorough rinse ^ 5, 2-24 hrs. -^ water, 



wash — > A, 1-5 mins. — > wash — > abs. ale, minimum possible time —^ xylene — > clove 



oil till differentiated — >• xylene -^ balsam 

 result: nuclei and some cell inclusions, blue black; keratin, orange-yellow: collagens, 



red. 



13.7 Ehrlich test. 1905 Lee cit. Grubler indulin-auraniia-eosin Y 



Lee 1905, 218 

 formula: glycerol 90, aurantia 6, indulin 6, eosin Y 6 

 preparation: Digest at 40°C. till completely dissolved. 

 method: [smears] ^ stain, 4-5 hrs., 40°C. ^ water, thorough rinse —> balsam, via 



acetone and xylene 

 result: nuclei, deep blue; some cytoplasmic inclusions, violet; plasma in general, 

 orange. 



13.7 Fraenkel see DS 21.13 Fraenkel (1928) 



13.7 Freeborn 1888 picro-nigrosin 645, 9:231 



formula: water 100, nigrosin 0.05, picric acid 0.4 



method: [sections] — > stains, 5-10 mins. — > wash — > balsam, via usual reagents 

 result: nuclei, black; collagens, blue; other tissues, yellow. 



13.7 Gomori 1946 see DS 21.423 Gomori 1946 



13.7 Hruby 1933 see DS 22.12 Hruby 1933 



13.7 Koneff 1938 see DS 21.421 Koneff 1938 



13.7 Kornhauser 1943 Quad Stain— auct. 20540b, 18 :95 



reagents required: A. 95% ale. 90, water 10, nitric acid 0.4, orcein 0.4; B. water 

 100, aluminum sulfate (cryst.) 10, ferric chloride 0.8, acid alizarin blue 2 B 0.35; C. 5% 

 phosphotungstic acid; D. water 100, acetic acid 2, orange G 2, fast green FCF 0.2 

 preparation of B: Boil the dye in the sulfate solution 10 minutes. Add the ferric chlo- 

 ride dissolved in a little water. 



