370 METHODS AND FORMULAS 



DS 13.7 



method: [sections of mercuric, or mercuric-chromic, fixed material after removal of 

 mercury by iodine treatment] ^ 85% ale. ^/l, 2-24 hrs. -^85% ale, thorough 

 wash -^ water, via graded ales. -^ B; 5-10 mms. -^ rmse -^ C, till collagen destained 

 -> rmse -^ D, 10 mms. -^ 50% ale, wash -^ balsam, via usual reagents 



result: elastic fibers, brown; nuclei, blue; cytoplasm and muscle, violet; collagen, green; 

 erthyrocytes, orange. 



13.7 Kornhauser 1945 20540b, 20:33 



REAGENTS REQUIRED: A. as Kornhauser 1943 above; B. water 100, acetic acid 0.475, 

 sodium acetate 0.023, acid alizarin blue 2 B 0.35, ammonium alum 5; C. water lOo' 

 phosphotungstic acid 4, phosphomolybdic acid 1; £). as Kronhauser 1943 above 

 method: [material as for Kornhauser 1943 above] -» 85% ale, -^ A, 2-24 hrs. -^85% 

 ale, thorough wash -^ water, via graded ales. -^ B, 5-10 mins. —> rinse ^ C, 10-30 

 mins. -^ quick rinse -^ D, 10 mins. -^ 50% ale wash -^ balsam, via usual reagents 

 result: As Kornhauser 1943 above. 



13.7 Krugenberg and Thielman 1917 23632,34:234 



REAGENTS REQUIRED: .4.. water 108, anilin blue 0.45, eosin'^. 0.22, phloxine 0.45 

 PREPARATION OF A: The eosins are each dissolved in 45 water and mixed with the blue 



dissolved in 18. 

 method: [sections of alcohol-fixed material] -^ A, 2-10 mins. -* rinse ^ abs. ale, 



minimum possible time -^ balsam, via usual reagents 



13.7 Lillie 1945a 4349^ 25 :27 



reagents required: A. water 99, acetic acid 1, brilliant purpurin R 0.6, azofuchsin 



G 0.4; B. 1% acetic acid; C. water 100, picric acid 1, naphthol blue black 1 

 method: [sections, nuclei stained by DS 11.121 Weigert 1903] ^ water -^ A, 5 mins. -* 



B, rinse -* C, 5 mins. -^ B, 2 mins. -^ balsam, via usual reagents 



result: collagen, reticulum, basement membranes, dark green; muscle and glands, 

 brown; erythrocytes, brownish red. 



13.7 Lillie 1945b 4349^ 25 :28 



REAGENTS REQUIRED: A. either 0.1% fast green FCG in 1% acetic acid or 0.3% wool 



green FCF in 1% acetic acid; B. 1% acetic acid; C. either 0.2% acid fuchsin or 0.2% 



violamine R 

 method: [sections, nuclei stained in DS 11.122 Lillie 1948] -^ water -^ A, i mins. -^ B, 



wash -^ C, 10-15 mins. -^ B, 2 mins. -^ balsam, via usual reagents 

 result: connective tissue, red; erythrocytes, green; muscle and cytoplasm, gray-green. 



13.7 Lillie 1945c 4349^ 25 :32 



REAGENTS REQUIRED: ^.1% eosiu Y; B. 3% ferric chloride; C. 1% naphthol green B; 



D. 1% acetic acid 

 method [sections, nuclei stained by DS 11.121 Weigert 1903] -^ water -^ A, 3 mins. -^ 



rinse -^ .B, 4 mins. -^ rinse ^ C, 5 mins. -^ D, 2 mins. -^ balsam, via acetone and 



xylene 

 result: general cytoplasm, pink; collagen, green. 



13.7 LiUie 1945d 4349, 25 :32 



REAGENTS REQUIRED: A. 1% Biebrich scarlet in 1% acetic acid; B. 1% acetic acid; C. 



0.5% methyl blue in 0.3% hydrochloric acid 

 method: [sections, nuclei stained by DS 11.121 Weigert 1903]^ waters A, 5 mins. 



— > S, 2 mins. —* C, 5 mins. -^ balsam, via usual reagents 



13.7 Lillie 1945e 4349, 25:41 



REAGENTS REQUIRED: A. 1% phloxine B; B. 1% acetic acid; C. water 100, hydrochloric 



acid 0.25, methyl blue 0.1, orange G 0.6 

 method: [sections with nuclei stained by DS 11.121 Weigert 1903]^ water —> A, 10 

 mins. —> B, 2 mins. -^ C, 10 mins. —* B, 5 mins. — > balsam, via acetone and xylene 



13.7 Lonnberg 1891 carmine-spirit blue 20796,6:1 



REAGENTS REQUIRED: A. Any DS 11.22 formula; B. 0.1% hydrochloric acid in 70% ale; 



C. sat. 60%, ale sol. spirit blue 100, hydrochloric acid 1 drop; D. 85% ale adjusted 

 with ammonia to pH 8 



