DS 13.7 DYE STAINS OF GENERAL APPLICATION 371 



method: [whole objects] — > A, till stained — > B, till differentiated -^ 70% ale, wash — ♦ 



C, 15 mius. -~f D, till violet --> balsam, via usual reagents 

 RECOMMENDED FOR: double staining wholemounts. 



13.7 Lopez 1946 Tech. Bull., 7 :53 



REAGENTS REQUIRED: A. water 100, DS 11.43 Ziehl 1882 10, acetic acid 0.2; B. water 100, 

 40% formaldehyde 4, acetic acid 0.2; C. water 100, phosphomolybdic acid 1, anilin 

 blue WS 0.5, methyl orange to sat. 

 PREPARATION OF c: Dissolve the acid with gentle heat. Add the anilin blue. After com- 

 plete solution, add an excess of orange. Warm a few minutes. Filter. 

 method: [sections of formaldehyde material] —> water -^ ^, 1 min. — > wash — » B, 3 

 mins. — + wash -^ C, 3-^-1 min. —> quick rinse —>■ abs. ale, least possil)le time — » bal- 

 sam, via xylene 



13.7 Lynch 1930 carviine-indulin 23632, 46 :465 



reagents required: A. DS 11.22 Grenacher 1879; B. hydrochloric acid; C. 0.5% hydro- 

 chloric acid in 70% ale; D. sat. sol. indulin in 80% ale. 



method: [whole objects, preferably mercuric-fixed]^ A, till thoroughly saturated-^ 

 add B, drop by drop, till brick red ppt. formed; leave 12 hrs. -^ C, till object clear 

 pink -^ add D, drop by drop till solution faint blue, leave till blue round edges ^95% 

 ale, 1 hr. -^ balsam, via usual reagents 



result: thick or dense, structures, red; thin or diffuse structures, blue. 



recommended for: double-staining wholemounts. 



13.7 Mallory 1900 see DS 11.124 Mallory 1900 



13.7 Mann 1892 see DS 13.7 Mann 1894a (note) 



13.7 Mann 1894a jnethyl hlue-eosin 23632, 11 :490 



reagents required: A. water 55, 1% methyl blue 20, 1% eosin 25; B. 0.005% potas- 

 sium hydroxide in abs. ale; C. 1% eosin 

 method: [smears or sections] -^ water -^ A, 24-48 hrs. -^ water, rinse — > abs. ale, till 

 dehydrated -^ B, till red -^ abs. ale, thorough wash — > water — » C, till differentiated 

 -^ balsam, via usual reagents 

 note: Alzheimer 1910 (Nissl and Alzheimer 1910, 409) gives A as water 150 to 35 of 

 each of the dye solutions. Langeron 1942, 598, attributes this formula, without refer- 

 ence, to "1892." Perdrau 1939 (11431, 48:009) recommends mordanting in 2% 

 ammonium molybdate before this method. 



13.7 Mann 1894b toluidine blue-erythrosin 23632, 11 :489 



reagents required: A. water 100, erythrosin 0.1; B. water 100, toluidine blue 1.0; 



C. 0.2% acetic acid 

 method: [water] -^ A, 1-2 mins. — > rinse -^ B, on slide, 1-2 rains. -^ C, till differenti- 

 ated -^ balsam, via usual reagents 

 result: nuclei, cartilage, blue; other structures, polychrome red and violet. 



13.7 Masson test. 1942 Langeron erythrosin-toluidine blue-orange G 



Langeron 1942, 613 

 reagents required: A. ADS 12.2 Lugol (1905); B. 5% sodium thiosulfate; C. water 

 100, erythrosin 0.2, orange G 1; D. water' 100, toluidine blue 1; E. 0.2% acetic acid 

 method: [sections of F 3700.1000 Helly 1903, F 7000.1000 Orth 1896. or F 3700.0010 

 Zenker 1894 fixed material] — > water — > A, 30 mins. — > jB, 2 mins. -^ thorough wash 

 -^ C, 1-2 mins. — > rinse — > D, on slide, 1-2 mins. -^ E, till differentiated —> balsam, 

 via usual reagents 

 result: as 13.7 Mann 1894b but with bacteria deep blue. 



13.7 Matsura 1925 polychrome neutral red 8542a, 3:107 



reagents required: A. 1% congo red in 95% ale; B. 1% phosphomolybdic acid in 



abs. ale 

 method: [sections] ^^ a, 12-24 hrs. -^ abs. ale, rinse —* B, 5 mins. —> abs. ale, till 



differentiated — > neutral mountant, via oil of thyrne 

 result: nuclei, red; elastic fibers, red violet; collagen, green; white blood cells, violet; 



other tissues, brown. 



