372 METHODS AND FORMULAS DS 13.7 



13.7 Merbel 1877 carmine-indigo carmine 645,1:242 



STOCK solutions: I. water 100, sodium borate 7, carmine 1.7; II. water 100, .sodium 



borate 7, indigocarmine 7 

 PREPARATION OF STOCK SOLUTIONS: Boil 15 mimites. Cool. Filter. 

 REAGENTS REQUIRED: A. stock I 50, stock II 50; B. sat. sol. oxalic acid 

 method: [sections of F 7000.0000 Miiller 1859 material]-^ water-* A, 15-20 mins. -^ 



B, till differentiated -^ wash — » balsam, via usual reagents 



13.7 Norris and Shakespeare lest. 1895 Rawitz carmine-indlgocarmine 



Rawitz 1895, 67 

 formula: water 130, sodium borate 8, carmine 1, indigocarmine 4 

 preparation: Boil the carmine in 65 water with 4 borax for 15 minutes. Cool. Filter. 

 Boil indigocarmine in 65 water with 4 borax 5 minutes. Cool. Filter. Mix filtrates. 

 Filter. 



13.7 Petersen 1924 test. 1946 Roskin Roskin 1946, 230 



reagents required: A. water 100, aluminum sulfate 10, acid alizarin blue 0.5; B. 5% 



phosphotungstic acid; C. water 100, acetic acid 8, orange G 2, anilin blue 0.5 

 method: [sections] —> water —♦ a, 5 mins. —» rinse -h. i?, several minutes —> distilled 



water, rinse — > C, 2 mins. -^ rinse -^ abs. ale, till dehydrated -+ balsam, via xylene 



13.7 Plehn test. 1896 Kahlden and Laurent methyl blue-eosin 



Kahlden and Laurent 1896, 126 

 formula: water 20, sat. aq. sol. methyl blue 60, 0.5% eosin in 70% ale. 20, 20% sodium 

 hydroxide 0.5 



13.7 Proescher, Zapata and McNaught 1946 Tech. Bull., 7:50 



STOCK solutions: I. 0.1% azophloxine; II. DS 11.4 anonymous 1936 

 working solution: stock I 60, stock II 30 

 method: [frozen sections of hot-formaldehyde-fixed materials]—* stain, 10-30 sees. — > 



abs. ale, least possible time for dehydration -^ M 23.1 mountant 

 recommended for: rapid staining for diagnosis. 



13.7 Roskin 1946 Roskin 1946, 231 



REAGENTS REQUIRED: A. 70% alc. 100, hydrochloric acid 1, orcein 1; B. any DS 11.122 

 formula; C. DS 12.221 van Gieson 1896 100, acetic acid 0.3; D. water 100, C (preced- 

 ing) 4 

 method: [sections] —> water —> a, 1 hr. -^ wash, 3 mins. -^ B, till thoroughly over- 

 stained — > distilled water, 3 mins. -^ running water, 3 mins. -^ distilled water, 3 mins. 

 — » C, 5 mins. -^ D, quick rinse — » blot — * abs. ale, minimum possible time — > balsam, 

 via xylene 



13.7 Schleicher 1943 Tech. Bull, 4:35 



REAGENTS REQUIRED: A. 0.1% azocarmine B in 5% acetic acid; B.b% phosphotungstic 



acid; C. water 100, acetic acid 0.3, orange G 0.13, anilin blue 0.07 

 method: [sections of F 3700.1000 Helly 1903 fixed material with mercury removed by 



iodine treatment] -^ A, 15-30 mins., 56°C. — » rinse — > B, 15-30 mins. till nuclei deep 



carmine in pink cytoplasm^ rinse—* C, 15-30 mins. — » rinse—* 95% ale, till color 



clouds cease -^ balsam, via usual reagents 



13.7 Schmorl 1928 see DS 21.13 Schmorl 1928 



13.7 Shumway 1926 magenta-picro-indigocarmine 20540b, 1 :37 



REAGENTS REQUIRED: A. sat. sol. (circ. 1%) magenta; B. sat. sol. (circ. 0.2%) indigo 



carmine 50, sat. sol. {circ. 1.2%) picric acid 50 

 method: [sections] -^ water —* a, 20 mins. -^ B, 5 mins. —* 70% alc. till pink, few 



sees. — * abs. ale, till blue green, several sees. -* balsam, via xylene 

 result: resting nuclei, dark blue; mitotic figures, dark red; cartilage, pink; procartilage, 



light blue; bone, dark blue; muscle, bright green; nerves, purple. 



13.7 Twort test. Minchin 1909 neutral red-light green 17510, 53:755 



preparation of dry stock: DUute 50 sat. sol. (circ. 3%) neutral red to 100. Dilute 

 50 sat. sol. {circ. 20%) light green to 100. Mix at 50°C. Cool. Filter. Wash and dry 

 ppt. 



