DS 13.7 DYE STAINS OF GENERAL APPLICATION 373 



WORKING solution: water 70, propanol 30, dry stock 0.5 



method: [sections from F 0000.0010 or F 7000. ioOO fixed material] -^ water -^ A, 40°C., 



10 mins. — > distilled water, rinse -+ abs. ale, till differentiated — ♦ balsam, via xylene 

 result: nuclei, purple; blood, green; connective tissues, blue green. 

 note: see also DS 23.3 Twort 192-1. Langeron 1942, 565 recommends the addition of 1% 



phenol to the working solution. 



13.7 Volkman and Strauss 1934 azocarmine-naphlhol green-crystal violet 



23632, 51 :244 

 REAGENTS REQUIRED: A. DS 21.13 Volkman and Strauss 1933 {see DS 21.13 Weigert 

 1898 — note); B. 0.1% azocarmine in 1% acetic acid; C. 0.1% aniline in 95% ale; D. 

 5% phosphotungstic acid; E. 1% naphthol green B in 1% acetic acid 

 method: [sections] — > water —* A, 1-2 hrs. — > 70% ale, rinse —* wash —* B, 30 mins. — » 

 — > C, quick wash — > D, till collagen decolorized — » wash — + E, 15 mins. -^ wash — » 

 abs. ale, least possible time — > balsam, via xylene 

 result: nuclei and muscles, red; collagen, green; elastic fibers, black. 



13.7 Westphal 1880 test. Bohm and Oppel carmine-crystal violet 



Bohm and Oppel 1907, 193 

 formula: water 40, abs. ale. 40, glycerol 20, acetic acid 4, ammonium alum 0.4, car- 

 mine 0.4, phenol 0.4, crystal violet 5 

 preparation: Boil the carmine and alum in the water 15 minutes. Filter. Dissolve the 

 crystal violet in the ale Add to cooled filtrate. Leave overnight. Filter; add the 

 glycerol and acid. 



13.7 Williams 1935 11284,20:1185 



preparation of stain: Dissolve 1 cresj'l violet and 1 potassium carbonate (anhyd.) in 

 95 water and 5 40% formaldehyde. Shake for 30 minutes. Add slowly and with con- 

 stant agitation, 3 acetic acid. Shake 30 minutes. Filter and add 5 iospropyl ale 

 method : [frozen sections of unfixed tissues] — + stain, 6 sees. — > wash -^ examine 

 result: nuclei and muscle, blue; fat, yellow; other tissues, pink. 



