21 



Formulas and Techniques for Dye Stains of 

 Special Application 



Decimal Divisions Used in Chapter 



DS 20 DYE-STAINING TECHNIQUES OF SPECIAL APPLICATION 

 21 Selective dye stains for histological elements 



21.1 Special stains for skeletal tissues 



21.10 Typical examples 



Preparation of a wholemount of a small salamander with the 

 skeleton stained by the alizarin technique of Gray 1929 

 Preparation of an embryo salamander to show the cartilagenous 

 skeleton by the method of van Wijhe 1902 

 Preparation of a transverse section of a root using the acid fuch- 

 sin-iodine green technique of Chamberlain 1915 



21.11 Bone and calcified tissues 



21.12 Cartilage 



21.13 Elastic fiber 



21.14 Chitin 



21.15 Plant skeletal tissues 



21.16 Other skeletal and connective tissues 



21.2 Special stains for nervous tissues 



21.20 Typical examples 



Preparation of a transverse section of the brain of a frog using 



the stain of Bethe 1896 



Preparation of a section of spinal cord using the "Weigert- 



Pal" stain of Anderson 1922 



Demonstration of the neuroglial cells of the white matter of the 



cerebral cortex using the crystal violet stain of Galescu 1908 



21.21 Nerve cells and processes 



21.211 Methylene blue and toluidinc blue methods 



21.212 Hematoxylin methods 



21.213 Other methods 



21.22 Methods for neuroglia 



21.23 Methods for other nervous elements 



21.3 Special stains for blood 



21.3 Special stains for other tissues 



21.41 Designed to differentiate types of tissue not covered under 

 21.1, 21.2, or 21.3 



21.411 Plant tissues 



21.412 Animal tissues 



21.42 Designed to differentiate types of cells 



21.421 In pituitary 



21.422 In other glands 



21.423 In other structures 



374 



