DS21.il DYE STAINS OF SPECIAL APPLICATION 385 



21.11 von Recklinghausen tcit. 1928 Schmorl Sohmorl 1928, 2(;0 



REAGENTS REQUIRED: A. 0.1% tliioiiiiie; B. sut. sol. phosphoinolybdic acid in glycerol 



C. 2% hydroquinone in glycerol; D. 3% phenol in sat. sol. potassium alum.; E. sat. 



sol. hydroquinone; F. anhydrous glycerol; G. toluene 60, abs. ale. 30 

 method: [hand or ground sections of nondecalcified bone preserved in P12.3 Kaiserling 



1897] — > A, till deep blue -^ rinse — > B, overnight — > C, till no more color comes away 



-^ D, 1-2 hrs. -^ thorough wash —> E, thorough wash -^ wash — > F, till dehydrated 



-^ G, till glycerol removed —y balsam, via toluene 

 RECOMMENDED FOR: general bone structure. 



21.11 Roehl kd. 1933 Cajal and de Castro Cajal and de Castro 1933, 314 



REAGENTS REQUIRED: A. coppcr tetramino solution (see note below); B. DS 11.121 



Weigert 190-4; C. ADS 21.1 Weigert 1885 50, water 50 

 method: [paraffin sections]^.!, 5 mins. -^ wash -^ 7^, 15 uiiiis. —> rinse —> C, till 



differentiated -^ wash -^ balsam, via usual reagents 

 recommended for: pathological calcareous deposits. 

 note: Copper tetramine is prepared by adding sufficient ammonium hydro.xide to a 



solution of a copper salt to redissolve the ppt. first formed; neither Cajal and de 



Castro {loc. cit.) nor Schmorl 1928, 196 specify the strength of the solution. 



21.11 Schmorl 1928a Schmorl 1928, 265 



reagents required: A. 0.1% thionin; B. sat. sol. phosphotungstic acid; C. 20% 



formaldehyde 

 method: [paraffin sections of decalcified bone]—* water, thorough wash -^ A, 5 mins. 

 —> wash ^95% ale, 1-2 mins. —> wash ^ 5, till differentiated, few moments—* 

 wash -^ C, 1-2 mins. ^95% ale. -^ balsam, via usual reagents 

 recommended for: general bone structure. 



21.11 Schmorl 1928b Schmorl 1928, 266 



reagents required: A. 0.1% thionin; B. sat. sol. phosphotungstic acid; C. 5% potas- 

 sium alum 

 method: [paraffin sections of bone fixed in formaldehyde, hardened in F 7000.0000 

 Muller 1850 and decalcified in AF 21.1 Schmorl] — > water, thorough wash — * A, 10- 

 30 mins. -^ wash — > 95% ale, 1-3 mins. -^ wash -^ B, 10-25 mins. — * wash, 2 hrs. 

 -^ C, 1-2 hrs. — > running water, 3-12 hrs. — >• balsam, via usual reagents 

 recommended for: demonstration of lamellae. 



21.11 Schmorl 1889 23681,10:745 



reagents required: A. DS 11.44 NicoUe 1871; B. sat. sol. picric acid 



method: [celloidin sections] -^ water —» A, 5-10 mins. -^^ wash —> B, J2~l nain. -^ 



thorough wash— » 70% ale. till no more color comes away —> balsam, via usual 



reagents 

 result: pro-cartilage, yellow; cartilage, blue; bone, brown black. 



21.11 True 1947 20540b, 22:107 



REAGENTS REQUIRED: A. Water 100, potassium hydroxide 5, hydrogen peroxide (3%) 



0.1; B. 0.01% alizarin red S in 2% hydroxide 

 method: [vertebrate embryos] —* yl, till bones clearly visible —♦ /?, till bones red -^ 



glycerol, via graded series 

 RECOMMENDED FOR: boncs in wholemounts. 



21.11 Weil test. cirr. 1938 Wellings Wellings circ. 1938, 156 



REAGENTS REQUIRED: .1. sat. aq. sol. uiercuric chloride; B. 0.1% iodine in 90% ale; C. 

 DS 11.22 Grenacher 1879; D. 1 % hydrochloric acid in 70% ale; E. 40% dried Canada 

 balsam in chloroform 

 method: [1 mm. slices of tooth] —> A, 6-8 hrs. — > wash -^ 70% ale via graded ales. — » 

 B, 12 hrs. — > abs. ale, till decolorized — > water, till rehydrated — > C, 3-7 days — » D, 

 24 hrs. --> al)S. ale, via graded ales., till ilcliydratcd » chloroform, 24 hrs. — > E, till 

 impregnated — > evaporate till hard — > [grind section (see Chapter 10)] 

 RECOMMENDED FOR: structurc of dentine.' 



