388 METHODS AND FORMULAS DS 21.13 



21.13 Goldmann test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 144 



REAGENTS REQUIRED: A. sat. alc. sol. Crystal violet; B. 0.01% potassium hydroxide in 



95% alc. 

 method: [sections]-^ A, 24 hrs. — ^> 95% alc, rinse —> B, till differentiated-^ balsam, 



via usual reagents 



21.13 Gomori 1950 aldchyde-fuchsin—auct. Tech. Bull, 20 iQQS 



formula: water 30, 95% alc. 70, magenta 0.5, hydrochloric acid 1, paraldehyde 1 

 method: [paraffin sections] — » 95% alc. — * stain (aged at least 24hrs.) 5-10 mins. — > 95% 



alc, wash — ^ balsam, via usual reagents 

 note: This stain will also differentiate mast cells, cells of the pancreas (15-20 minutes 



in stain), certain cells in the pituitary (30 minutcs-2 hours in stain). 



21.13 Hart 1908 23681, 19:1 



reagents required: A. DS 11.28 Orth (1892); B. water 30, 95% alc. 70, DS 21.13 



Weigert 1898 (working sol.) 5, hydrochloric acid 1 

 method: [sections] -^ water -^ a, 2-5 mins. — > J5, 12-15 hrs. —> wash -* balsam, via 

 usual reagents 



21.13 Hart 1908 23681, 19:1 



REAGENTS REQUIRED: A. 0.25% potassium permanganate; B. 5% oxalic acid; C. water 



75, 95% alc, 25, DS 21.13 Weigert 1898 (working sol.) 5, hydrochloric acid 1 

 method: [sections] —> A, 10 mins. —* wash — > B, 20 mins. — > wash -^ C, overnight -^ 



95% alc, rinse -^ wash — > counterstain, if desired — > balsam, via usual reagents 



21.13 Herxheimer 1886 8645, 4:785 



REAGENTS REQUIRED: A. abs. alc 50, hematoxylin 2.5, water 50, lithium carbonate 0.04; 



B. 20% ferric chloride 

 method: [sections] — > water -^ A, 5 mins. to 1 hr. — > rinse -^ B, till fibers differentiated 

 — >• wash -^ balsam, via usual reagents 



21.13 Krajian 1934 1789a, 18 :378 



reagents required: A. 2% aluminum chloride; B. water 80, sodium citrate 4, neutral 



red 3, glycerol 20; C. ADS 12.2 Lugol (1905); D. water 100, anilin blue 1.5, orange G 



2.5, resorcinol 3, phosphomolybdic acid 1 

 method: [sections of formaldehyde-fixed material] — > A, 10 mins. — > water, rinse — > B, 



30 mins. — > water, rinse — > C, 30 sees. -^ water, rinse — > D, 30 mins. -^ water, rinse 



— > abs. alc, minimum possible time —^ balsam, via oil of thyme and xylene 

 result: elastic fibers, bright red; other connective tissues, dark blue. 



21.13 Kultschitzky 1896 1780, 46 :675 



formula: 95% alc 100, water 5, potassium carbonate 0.05, magdala red 2, methylene 



blue 1 

 use: elastic fibers in sections of F 7000.0000 Mliller 1859 fixed material 

 note: Conn 1946, p. 112 points out that Kultschitzky {loc. cit.) may have used phloxine 

 rather than magdala red. 



21.13 Mallory 1938 see DS 22.8 Mallory 1938 



21.13 Manchot test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 144 



reagents required: A. sat. aq. sol magenta; B. water 100, sucrose 100, sulfuric acid 2 

 method: [sections] -^ water ^^ A, 30 sees. — > rinse -^ B, till differentiated M 11 or 12 

 mountant 



21.13 Pfitzner 1887 23632, 4:82 



formula: abs. alc. 100, safranin 10 

 method: [sections of chrome-fixed material] -^ stain, 48 hrs., 40°C. — > water, wash -^ 



balsam, via usual reagents 

 result: elastic fibers black on gray. 

 note: This may be u.sed only after chrome fixation. 



21.13 Pranter t^st. 1905 Hall and Herxheimer Hall and Herxheimer 1905, 86 



formula: water 30, 95% alc. 70, nitric acid 2, orcein 0.1 

 method: as Unna-Taenzer (1896) 



