DS 21.13 DYE STAINS OF SPECIAL APPLICATION 389 



21.13 Romeis 1948 see DS 13.7 Romeis 1938 



21.13 Roskin 1946 see DS 13.7 Roskin 1946 



21.13 Rubens-Duval lest. 1938 Carleton and Leach Carleton and Leach 1938, 220 



formula: water 70, 95% ale. 30, nitric acid 10, orcein 0.1 

 method: water — > stain, 21 hrs. — > wash — > balsam, via usual reagents 



21.13 Schmorl 1928a S(;hinorl 1928, 168 



REAGENTS REQUIRED: A. DS 21.13 Wclgcrt 1898 (working sol.); B. DS 11.121 Weigert 



1904; C. DS 12.221 Weigert 1904 

 method: [sections] -^ water —» A, '2-1 l^f- -^ 95%, till differentiated, '^-l Jif. — > water 

 — » B, 5-10 niins. — > wash -^ C, till ditTerentiated — > balsam, via usual reagents 



21.13 Schmorl 1928b Schmorl 1928, 168 



REAGENTS REQUIRED: A. DS 11.28 Orth (1892); B. DS 21.13 Weigert 1898 (working sol.) 



CDS 21.41 Weigert 1887 (.4 solution); D. 0.6% hydrochloric acid; ^. ADS 11.1 Gram 



1880; F. aniline 60, xylene 30 

 method: [sections] —> waters A, 2-5 mins. — ♦ 95% ale, wash — » B, 10-30 mins. -^ 



95% ale. till differentiated — » wash — >• C, 5-10 mins. -^ D, wash —»■£', 5 mins. — * blot 



— » F, till differentiated -^ balsam, via xylene 

 recommended for: differentiation of elastic fibers in tissues containing Gram-positive 



bacteria. 

 note: Bismarck brown may be substituted for magenta in the preparation of B above. 



21.13 Schmorl 1928c Schmorl 1928, 168 



REAGENTS REQUIRED: A. DS 11.43 Zichl 1882; B. DS 21.13 Weigert 1898 (working sol.); 



C. 1 % methylene blue 

 method: [sections] -^ water —* A, 1 hr., 37°C. -^ 70% ale, wash -^ B, 20-30 mins. — > 

 abs. ale. till differentiated — > water -^ C, 5-10 mins. — > wash — ♦ balsam, via usual 

 reagents 

 recommended for: differentiation of elastic fibers in tissues without destaining acid- 

 fast bacteria. 



21.13 Schmorl 1928d Schmorl 1928, 169 



reagents required: A. DS 11.28 Orth (1892); B. 1% hydrochloric acid in 70% ale; 

 C. sat. sol. crystal violet in sat. sol. aniline; 1). Vesuvelin (see DS 21.13 Weigert 1898, 

 note) 

 method: [sections] — > water -^ A, 10-20 mins. — > B, till differentiated, 10-20 mins. — > 

 wash -^ C, 1 hr., 37°C. — > 95% ale, till no more color comes away — » D, 20-30 mins. 

 -^95% ale, wash — > abs. ale, till differentiated —* balsam, via xylene 

 recommended for: differentiation of elastic fibers in tissues without destaining acid- 

 fast bacteria. 



21.13 Sheridan 1929 11571b, 12:103 



formula of dry stock: water 100, crystal violet 1, resorcinol 2, 30% ferric chloride 9 

 preparation: as French 1929 



working solution: Prepare from ppt. exactly as for French 1929 above. 

 method: [sections] ^90% ale — > stain, 1-2 hrs. —* abs. ale till differentiated—* bal- 

 sam, via xylene 

 result: as French 1929 but much paler. 



21.13 Unna test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 143 



reagents required: A. sat. aq. sol. Bismarck brown; B. 50% ale 100, magenta 1, 



nitric acid 5; C. 25% nitric acid; D. 0.01% acetic acid 

 method: [sections] -^ water — > A, some hours to overnight —* wash — » B, 24 hrs. — > C, 

 quick dip — » D, till differentiated -^ balsam, via usual reagents 



21.13 Unna-Taenzer test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 144 

 reagents reqitired: ^4. 50% ale 100, orcein 0.8, hydrochloric acid; B. 0.1% hydro- 

 chloric acid in 80% ale. 

 method: sections-^ water—* A, 6-12 hrs. — > B, till differentiated—* balsam, via usual 

 reagents 



