DS 21.15 DYE STAINS OF SPECIAL APPLICATION 393 



result: lignified tissues, green; other tissues, red. 



note: Laugeron {loc. cil.) refers to tliis as la inMhoilc clasxiiiuc Johansen 1940, IKi, uses 

 1% acid fuchsin as the red counterstain iu tliis tec-huiquc; this modification is essen- 

 tially the method of Chamberlain 1915a. 



21.15 Langeron 1902 /rs/. 1942 ips. methylene blue-ruthenium red 



Langeron 1912, 12G7 

 REAGENTS RiOQUiUEu: .1. water lUO, i)otassiuni alum 10, methylene blue 1; B. 0.2% 



ruthenium red 

 method: [sections] -^ water — ♦ A, 5-10 mins. -^ water, wash -^ B, 5-10 mins. — > water, 



wash — > Ixilsam, via usual reagents 

 result: suberizcd tissues, green; sclerenchyma, violet; lignified tissues, blue; paren- 

 chyma, deep rose. 



21.15 Langeron see also DS 21.15 Bugnon 1919 (note) 



21.15 Margolena 1934 Bismarck brcnvn-fast green 20540b, 9:71 



reagents required: A. 0.5% Bismarck brown; B. 0.3% fast green FCF in clove oil 

 method: [sections of F 5000.1010 Bouin 1897 fixed buds] -^ A, 10 mins. —* rinse — > de- 

 hydrate — » B, till differentiated -^ balsam, via xylene 

 recommended for: Differentiation of walls of devcloi)ing pollen grains. 



21.15 Northen 1936 safranin-tannin-crystal violet 20540b, 11 :23 



reagents required: A. \% safranin in 50% ale; B. 0.5% tannin in 50% ale; C. 1% 



ferric chloride in 70% ale; D. 0.5% crystal violet in clove oil 

 method: [sections] — > water — »• .4, 24 hrs. -^ 50% ale, rinse -^ B, 30 sees. -^ 70% ale, 



two rinses -^ C, 10-20 sees. — > abs. ale, minimum possible time —> D, 30-CO sees. 



balsam, via xylene 

 result: lignified tissue, red; other tissues, black and purple. 



21.15 Petit 1903 alkanet-iodine green-chrome yellow 0630,55:507 



reagents required: A. DS 11.3 Guinard 1890; B. 0.01% iodine green in 95% ale; 



C. sat. sol. lead acetate; D. sat. sol. potassium dichromate 



method: [sections]-^ waters A, till suberized tissue red ^ 95% ale, rinse —> B, till 

 lignified tissues green —♦70% ale, rinse — > C, 5 mins. — > water, thorough wash -^ D, 

 few moments — > water, thorough wash -^ balsam, via usual reagents 



21.15 Popham, Johnson and Chan 1948 20540b, 23:185 



reagents required: A. 1% safranin O; B. 2% tannic acid; C. water 60, DS 11.122 

 Delafield (1885); D. 0.01% hydrochloric acid; E. 0.5% lithium carbonate; F. sat. sol. 

 anilin blue in methyl cellosolve 

 method: [sections] — ♦ water — > A, 24 hrs. — > rinse -^ B,2 mins. — > rinse — > C, 2 mins. — > 



D, wash -^ E, 5 mins. — ♦ dehydrate -^ F, 5-10 mins. -^ balsam, via usual reagents 

 recommended for: cell walls in stem apex. 



21.15 Sharman 1943 20540b, 18:105 



reagents required: A. 2% zinc chloride; B. 0.004% safranin O; C. water 100, hydro- 

 chloric acid 0.15, tannic acid 5, orange G 2; Z). 5% tannic acid; E. \% iron alum 

 method: [sections] — ♦ water ^ A, 1 mm. -^ rinse — > J?, 5 mins. — > rinse -^ C, 1 min. -^ 



rinse -^ D, 5 mins. — * quick rinse —* E, 2 mins. — > balsam, via usual reagents 

 recommended for: cell walls of stem apex. 



21.15 Smith test. 1903 Cole acid green-carmine Cross and Cole 1903, 170 



reagents required: A. water 75, glycerol 25, acid green 0.1; B. DS 11.26 Smith (1903) 

 method: [sections] -^ water -^ A, 5-10 mins. -> wash — > B, 10-15 mins. — > 96% ale, 

 wash — > balsam, via clove oil 



21.15 Smith 1924 safranin-picro-anilin blue 19938,59:557 



reagents required: A. DS 11.42 Chamberlain 1915; B. sat. sol. (circ. 1.2%) picric acid 



78, sat. sol. {circ. 5%) anilin blue WS 22 

 method: [sections] —> A, 2-6 hrs. — > water, thorough wash -^ 95% ale, till differenti- 

 ated -^ B, 2 hrs. -^ abs. ale, quick rinse — > balsam, via clove oil and xylene 

 note: Johansen 1940, 82 substitutes his DS 11.42 Johansen 1940 for A above and cites 

 the technique without reference to Smith, as do Conn and Darrow 1946, IIa-11. 



