394 METHODS AND FORMULAS DS 21.16 



21.16 OTHER SKELETAL AND CONNECTIVE TISSUES 



21.16 Baird 1935 20540b, 10:35 



REAGENTS REQUIRED: A. 1% trj^pan blue; B. 2% neutralized formaldehyde; C. 1% 



methylene blue; D. \% acid fuchsin 

 method: [inject (for rat) 1 ml. A daily for 4 days] -^ [spread piece of subcutaneous con- 

 nective tissue on slide: dry till edges adhere] -^ B, ^ 2 to \ hr. — > wash — > C, 1 min. — » 

 wash — > D, 30 sees. -^ wash — > balsam, via acetone and xylene 

 RECOMMENDED FOR: Connective tissue spreads for class instruction. 



21.16 Bowell test. 1948 Verdcourt 20540b, 23:145 



REAGENTS REQUIRED: A. Water 99, acetic acid 1, potassium permanganate 16; B. sat. aq. 



sol. {circ. 10%) oxalic acid; C. 0.1% Hoffman's violet 

 method: [radulae isolated by potassium hydroxide treatment]-^ wash -^ A, 100 ml., 



till black -^ B, till decolorized — » wash — ^ C, till stained, 30 mins. -^ balsam, via 



usual reagents 

 RECOMMENDED FOR: radulae of mollusca. 



21.16 Daniell927 11360,47:253 



REAGENTS REQUIRED: A. 0.05% quiuone in abs. ale; B. abs. ale. 50, methyl salicylate 50 

 method: [crustacea in 70% ale] ^ abs. ale, till dehydrated —> A, overnight—^ B, till 



diffusion currents no longer visible -^ methyl salicylate 

 RECOMMENDED FOR: demonstration of muscles in wholemounts of crustacea. 



21.16 Dietrich test. 1928 Schmcrl Schmorl 1928, 273 



REAGENTS REQUIRED: A. 1% brilliant black 3B in 0.1% acetic acid; B. 1% safranin; 



C. methanol 

 method: [3-4 m sections of mercuric chloride fixed material] —> A, 1-5 mins. — + rinse -^ 



B, 3-10 mins. -^ drain — > abs. ale, till differentiated -^ C ^ balsam, via xylene 

 RECOMMENDED FOR: structure of heart muscle. 



21.16 Hueter 1911 test. 1948 Romeis Romeis 1948, 351 



REAGENTS REQUIRED: A. 10% phosphotungstic acid; B. DS 11.124 Hueter 1911 

 method: [sections] -^ water -^ A, 15-20 sees. — > quick wash -^ B, 3-5 mins. — > 50% 



ale, till differentiated -^ balsam, via usual reagents 

 RECOMMENDED FOR: collagcn fibers. 



21.16 Jasswoin 1932 23632, 49:191 



REAGENTS REQUIRED: A. 5% fcrric alum 75, 1% hematoxylin 25 



method: [sheets of neutralized formaldehyde-fixed material] -^ thorough wash -^ spread 

 on slide —> 95% ale, till dehydrated —> water, tiU rehydrated — >• A, 15-20 sees. — » 

 tap water, wash — ^ [counterstain if desired] —>■ balsam, via usual reagents 

 recommended for: collagen fibers in wholemounts. 

 note: See also DS 11.121 Yasvoin (1946). 



21.16 Karlson 1924 6630,90:1122 



REAGENTS REQUIRED: A. water 100, eosin 0.7, gallic acid 0.15; B. 3% pyrogallic acid; 



C 5% methylene blue 

 method: [sections] — > water -^ A, 5-20 mins. -^70% ale, till no more color comes away 



— > B, 24 hrs. —> wash -^ C, 15 mins. -^70% ale, tiU no more color comes away — * 



balsam, via usual reagents 

 RECOMMENDED FOR: differentiation of muscle (red) from other connective tissues. 



21.16 Miller 1933 11431,37:127 



REAGENTS REQUIRED: A. DS 22.21 Altmau 1898 (sol. A); B. 0.5% aurantia in 70% ale; 



C. 0.25% toluidine blue 



method: [sections of F 3700.1000 Helly 1903 material] — > water -^ A, on slide, warmed to 

 steaming, 2-4 mins. —> quick wash —^ C, 10-20 sees. — > rinse —> blot — » C, 10 sees. — > 

 methanol, till differentiated — > balsam, via usual reagents 



recommended for: striae in striated muscle. 



21.16 Perdrau 1921 see MS 33.1 Perdrau 1921 



