404 METHODS AND FORMULAS DS 21.212 



1926 (20540b, 1 :72) states that this can be avoided by leaving the sections for 12 hours in 

 80% alcohol between the staining and differentiating processes. In the technique of Olivo- 

 crona 1917, however, the mordant is incorporated with the staining mixture. In spite of the 

 numerous modifications which have been made, the methods of Weigert 1885 and Weigert 

 1894 are probably the easiest and simplest to employ, though the rationalization of Anderson 

 1922 is now almost universal in Europe. The numerous techniques which are given below are 

 offered less as suggestions to be followed than in the hope that they may tend to diminish the 

 confusion by assigning to the correct author the technique which he has invented. 



21.212 Anderson 1922 11977, 5:65 



REAGENTS REQUIRED: A. ADS 12.1 Auderson 1922; B. ADS 12.1 Weigert 1891; C. water 



to make 100, acetic acid 3, abs. ale. 10, hematoxylin 0.5, 2% calcium hypochlorite 3; 



D. F 7000.0000 Miiller 1859; E. ADS 21.1 Pal 1887 (A & B sols.) 

 PREPARATION OF c: Dissolve dye in ale. Add hypochlorite. Shake well. Dilute with water 



and add acid. 

 method: [15-20 m celloidin sections]^ A, 48 to 72 hrs. 38°C. -^ B, 10-30 mins. -^ 



wash -^ C, 1 hr., 50°C. -^ D, 10-20 mins. — * wash — » E, till differentiated -^ balsam, 



via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 

 note: a detailed description of the use of this technique is given under DS 21.20 above. 



21.212 Anderson 1929 see either DS 11.121 Anderson 1929 or DS 11.123 Anderson 1929. 



21.212 Anderson 1942 11431, 54:258 



reagents required: A. ADS 21.1 Anderson 1942; B. DS 11.113 Kultschitsky 1889; C. 

 2.5% potassium dichromate; D. ADS 21.1 Pal 1880 {A and B sols.); E. DS 11.21 

 Anderson 1926 

 method: [30 fi frozen sections]-^ A, 1 hr., 50°C. ^ wash -^ B, 30 mins., 50°C -* C, 

 2-3 mins. -^ wash -^ D (A sol.), 2 mins. -^ D (B sol.), 1 min. -♦ [repeat D (A) -^ D 

 {B) cycle till differentiated] -* wash -^ E, 45-60 mins., 50°C. -* rinse -^ 80% ale. -^ 

 balsam, via usual reagents 



21.212 Bolton 1898 11025, 32:245 



reagents required: A. 1% osmic acid; B. DS 11.113 Kultschitzky 1889; CADS 21.1 



Pal 1887 (A & B sols.) 

 method: [15 fi sections of formaldehyde-fixed material]-^ distilled water -^ A, few 



mins. -+ rinse — > 5, 3 to 24 hrs. —>■ rinse -^ C, till differentiated -^ balsam, via 



usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



21.212 Clark and Ward 1934 20540b, 9 :34 



REAGENTS required: A. 4% ferric alum; B. DS 21.212 Weigert 1885 (sol. C); C. ADS 



21.1 Pal 1887 {A and B sols.); D. sat. sol. lithium carbonate 

 method: [sections] -^ A, 2-24 hrs. -^ quick wash -^ B, 1-2 hrs. -^ quick wash -* A, till 



gray and white matter just distinguishable -^ C (A sol.), till brown -^ rinse -^ C (B 



sol.) till gray matter bleached-^ wash—* D, 5 mins. -^ wash -^ balsam, via usual 



reagents 



21.212 Donaggio 1939 1820, 22:171 



REAGENTS REQUIRED: A. ADS 12.2 Lugol ; B. DS 11.124 Donaggio 1904; C. ADS 21.1 



Pal 1880 (A and B solutions) 

 method: [20 fi sections from F 3700.0010 Zenker 1894 material]-^ water -^ A, few 



moments —> 90%, ale, 1 hr. -^ water, via graded ales. — > B, six hrs. -^ C, (A sol.), 1 



min. -^ C (B sol.), 1 min. -> [repeat C (A) --^ C (B) cycle till differentiated] -^ wash 



— > balsam, via usual reagents 

 recommended for: differentiation of anesthetized nerves (stained) from normal nerves 



(unstained). 



21.212 Fajerstajn 1901 16341, 1:189 



REAGENTS REQUIRED: A. 0.5% chromic acid; B. 1% hematoxylin; C. ADS 21.1 Pal 1887 

 (A and B sols.) 



