DS 21.212 DYE STAINS OF SPECIAL APPLICATION 405 



method: [frozen sections of formaldehyde-fixed material] —> water — > A,2i hrs. — > wash 

 -^ B, 24 hrs. -^ wash -^ C (B sol.), till decolorized -> [repeat C (A) -> C (B) till 

 differentiated] — > balsam, via usual reagents 



RECOMMENDED FOR: myelin sheaths. 



21.212 Gudden 1897 15058, 16:24 



REAGENTS REQUIRED: A. 0.5% chromic acid; B. water 90, 95% ale. 10, hematoxylin 1, 



10% nitric acid 0.5; C. ADS 21.1 Pal 1887 (A & B sols.) 

 METHOD [20 fj. celloidin sections of formalin-hardened material] —> A, 10 hrs. rinse—* 

 80% ale, till no more color comes away — » B, 6-24 hrs. — > wash — * C, (sol. A), 15-20 

 sees. -* C, (sol. B) few sees. — » [repeat C (A) ^ C (B) cycle till sufficiently differenti- 

 ated] -* balsam, via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



21.212 Hadjioloff 1928 .see DS 21.212 Pal 1887 (note) 



21.212 Howden 1936 see DS 21.212 Bolton 1898 (note) 



21.212 Kaiser 1893 15058, 12:364 



REAGENTS required: .4. F 7000.0000 Muller 1859; B. F 1700.0000 Marchi 1886; C. 

 water 50, 95% ale. 50, ferric chloride 25; D. DS 21.212 Weigert 1885 (sol. C); E. ADS 

 21.1 Pal 1887 (sols. A & B) 

 method: [whole brains or pieces] —> .4, till hardened (some methods) -^ 15 mm. slices 

 -* B, 1 wk. -> [25 M sections] -> C, 5 mins. -* wash -» D, 2 to 12 hrs. -^ E, till differ- 

 entiated -^ balsam, via usual reagents 

 recommended for: myelin sheaths. 



21.212 Knower 1930 see DS 11.113 Knower 1930 



21.212 Kozowsky 1904 see DS 21.212 Pal 1887 (note) 



21.212 Kultschitsky 1890 766,6:519 



reagents required: A. DS 11.113 Kultschitsky 1889; B. 1.5% lithium carbonate; C. 



ADS 21.1 Kultschitsky 1889 

 method: [15-20 n celloidin sections of F 4700.0000 Erlitzky 1877 hardened material] -> 



A, 1-24 hrs. —>■ B, till no more color comes away -* C, till differentiated — * wash -^ 



balsam, via usual reagents 

 recommended for: myelin sheaths. 



21.212 La Manna 1937 23632, 54:257 



reagents required: A. 30% ferric chloride; B. 0.5% ferric chloride; C. DS 11.121 



La Manna 1937 

 method: [paraffin sections of F 7800.1000 La Manna 1937 fixed material] -+ water — > A, 



1 hr. — > S, quick rinse — > C, 1 hr. -^ running water, overnight — > B, till differentiated 



— > thorough wash — > balsam, via usual reagents 

 recommended for: myelin sheaths. 



21.212 Landau test. 1924 Spielmeyer Spielmeyer 1924, 97 



reagents required: A. 3.5% ferric alum; B. 1% hematoxj-lin; C. "weak" hydrogen 



peroxide 

 method: [celloidin sections of formaldehyde fixed material]^ water —> A, 12-24 hrs. 



-» rinse -♦ B, 12-24 hrs. —>■ tap water, 1 hr. -♦ C, till differentiated -> balsam, via 



usual reagents 



21.212 Landau 1938 4285a, 15:181 



reagents required: A. ADS 12.1 Landau 1938; B. 10% ferric alum; C. 1%, hema- 

 toxylin D. ADS 21.1 Landau 1938 

 method: [large pieces of formaldehyde fixed brain tissue] —> thorough wash — > .4, 24 

 hrs., 25°-30°C. — * thorough wash -^ [15 m paraffin sections] — > water — > B, 3-6 hrs., 

 25°-30°C. -*■ quick rinse -> C, several hrs., 25°-30° -> thorough wash -^ D, till dif- 

 ferentiated —>■ thorough wash -* balsam, via usual reagents 



