406 METHODS AND FORMULAS DS 21.212 



21.212 Liber 1937 1887a, 24 :230 



REAGENTS REQUIRED: A. ADS 12.1 Weigcrt 1896; B. DS 11.113 Kultschitsky 1889; C. 



1% lithium carbonate; D. ADS 22.1 Pal 1887 (A and B sols.) 

 method: [paraffin sections of formaldehyde material] — > water — > A, 12 hrs. — > rinse — * 



B, 1 hr. —>■ rinse —y C, till blue — > thorough wash — > D (A sol.), few seconds — » D, 

 (B sol.), till differentiated — * balsam, via usual reagents 



21.212 Lillie 1948a Lillie 1948, 171 



REAGENTS REQUIRED: A. 4% ferric alum 50, 1% hematoxylin in 95% ale. 60; B. 0.5% 



ferric alum; C. ADS 21.1 Lillie 1948; D. 0.1% safranin in 1% acetic acid 

 method: [sections of dichromate fixed or mordanted material] —> 80% ale. — > A, 40 



mins., 50°-60°C. — * rinse —> B, 1 hr. -^ C, till blue —* wash — > D, 5 mins. — > balsam, 



via usual reagents 

 RECOMMENDED FOR: myelin sheatlis. 



21.212 Lillie 1948b Lillie 1948, 160 



reagents required: A. 2.5% potassium dichromate; B. DS 21.212 Weil 1928 (sol. A) 



C. 0.5% ferric alum; D. ADS 21.1 Lillie 1948; E. working solution of Sudan I pre- 

 pared as in DS 22.4 Lillie 1948 



method: [frozen sections of formaldehyde-fixed material] -+ A, 2-4 days — > wash — > B, 

 45 mins. 55-60°C. — > wash -^ C, tUl decolorized — > wash — > D, 10 mins. — > wash — > 

 E, 10 mins. — > wash -^ M.ll mountant 



21.212 Loyez iest. 1929 Anderson Anderson 1929, 65 



reagents required: A. 4% ferric alum; B. abs. ale. 10, hematoxylin 1, water 90, sat. 



aq. sol. lithium carbonate 2; C. 0.1% hydrochloric acid in 70% ale. 

 preparation of reagent B: Dissolve hematoxylin in water. Add ale. Then add car- 

 bonate solution. 

 method: [15-20 n sections] — > water — * A, 24 hrs. -^ rinse -^ B, 2-4 hrs. 50°C. -^ wash 

 -^ C, till differentiated — > balsam, via usual reagents 



21.212 MacConaill test. 1951 Gurr Gurr 1951, 36 



stock solutions: I. water 92, acetic acid 8, lead nitrate 2, acid fuchsin 0.5; II. water 96, 



acetic acid 4, hematoxylin 1 

 reagents required: A. stock I 50, stock II 50; B. water 100, ammonium molybdate 14, 



ammonium acetate 0.4 

 method: [6-12 n sections of formaldehyde-fLxed material] — > 30% ale. — » A, 5 mins. —* 



wash -^ B, 1-2 mins. -^ thorough wash — ^ balsam, via usual reagents 



21.212 Mallory 1936 608b, 12 :569 



reagents required: A. sat. aq. sol. (circ. 1%) lead chloride; B. water 90, ADS 12.2 



Lugol 10; C. 0.05% hematoxylin 

 method: [3 mm. slices of formaldehyde-fixed material] —>■ A, 6 wks. room temperature 

 or 1 wk. 37°C. -^ wash, 24 hrs. -> 80% ale, till required — > [8 /x sections] — > water —y 



B, 1 min. ^•95% ale, till color removed — > C, J^ to 1 hr. —* tap water, till blue 

 note: All solutions must be freshly prepared immediately before use. 

 recommended for: general neurological staining. 



21.212 Mitrophanov 1896 23632, 13 :470 



reagents required: A. ADS 12.1 Mitrophanov 1896; B. DS'll.llS Kultschitsky 1889; 



C. ADS 21.1 Weigert 1885 



method: [15-20 /t celloidin sections] -^ A, 24 hrs. 45°C. -^ rinse — ♦ B, 10 mins. — > rinse 



— » C, till differentiated 

 recommended for: myelin sheaths. 



21.212 Nelson test. 1930 Guyer Guyer 1930, 104 



reagents required: A. 1% potassium hydroxide; B. water 75, acetic acid 12, glycerol 



12, chloral hydrate 0.75; C. water 75, glycerol 12, DS 11.123 Ehrlich 1886 12, chloral 



hydrate 0.75 

 method: [alcohol-hardened small vertebrates]-^ A, till translucent—* B, 3 days — » C, 



7 days -^ B, till differentiated -^ glycerol 

 recommended for: staining nerves in wholemounts of small vertebrates. 



