DS 21.212 DYE STAINS OF SPECIAL ArrLICATION 407 



21.212 Neumen 1915 4349, 6:71 



REAGENTS REQUIRED: A. watcf 100, chroiTiic acid 5, potassium diclironiate 4; B. 3% 

 ferric chloride; C. sat. aq. sol. {circ. 7.5%) cupric acetate; D. sat. ale. sol. {circ. 35%) 

 hematoxylin; E. water 100, potassium ferrocyanide 7, sodium borate 0.5; F. sat. sol. 

 Lithium carbonate 



method: [celloidin sections] -^ water -^ A, 2-5 mins. —>■ wash -♦ B, 2-5 mins. ->■ wash 

 -^ C, 2-5 mins. -^ wash — > Z), 2-5 mins. -^ wash — > C (fresh sol.), till copious color 

 clouds are produced -> wash -> D, till differentiated -» wash -+ F, 2 mins. -» wash 

 — » balsam, via usual reagents 



21.212 Olivecrona 1917 23681,28:521 



REAGENTS REQUIRED: A. Water 30, 95% ale. GO, hematoxylin O.G, ferric chloride 0.15, 



hydrochloric acid 0.3; B. water 100, ferric chloride 0.8, hydrochloric acid 1.0; C. sat. 



sol. lithium carbonate 

 PREPARATION OF A: Add the dye dissolved in ale. to the iron and acid dissolved in water. 

 method: [sections by freezing technique] -^ 70% ale— >.4, 1 hr. — > wash — > B, till 



differentiated —^ wash -^ C, till blue -^ balsam, via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



21.212 Pal 1887 Weigert-Pal—compl. script. 23632, 6 :92 



REAGENTS REQUIRED: A. 5% potassium dichromate; B. DS 21.212 Weigert 1885 (sol. C) 

 C. ADS 21.1 Pal 1887 (.4 & B sols.) 



method: [pieces of brain] -+ .1, till white matter dark brown (some wks.) — > wash -* 80% 

 ale, till required — ♦ [section 15 /x to 20 p] -^ water -^ B, 2 hrs. to 1 day — » wash — > C 

 (sol. A), 15-20 sees. -^ rinse —>■ C (sol. B), few sees. -> [repeat C (A) — > C (B) cycle 

 till sufficiently differentiated] —> balsam, via usual reagents 



recommended for: myelin sheaths. 



note: Tschernyschew and Karusin 1897 (23632, 13:354) substitute Kultschitsky 1889 

 DS 11.113 for B above. Kozowsky 1904 (15058,23:1041) substitutes his ADS 21.1 for 

 C above. Hadjioloff 1928 (4285a, 5:431) counterstains in a sat. ale. sol. light green. 



21.212 Schroder 1930 23430, 166:588 



reagents required: a. ADS 12.1 Schroder 1930; B. DS 11.124 Schroder 1930; C. 0.25% 



potassium permanganate; D. water 100, oxalic acid 0.5, potassium sulfite 0.5; E. 0.02% 



lithium carbonate 

 method: [20-30 /x frozen sections of formaldehyde-fixed material) — > A, 1 day, 37°C. — » 



rinse -^ B, 12 hrs., 37°C. — > wash -^ C, 30 sees. -^ rinse -^ D, 1 min. —>■ thorough 



wash — » [repeat C ^> D cycle if differentiation insufficient] -^ £", 15 mins. -^ wash — ♦ 



balsam 

 recommended for: myelin sheaths. 

 note: Schroder 1939 (23430, 166:588) does not differ significantly from the above. 



21.212 Schultze test. 1933 Cajal and de Castro Cajal and de Castro 1933, 234 



reagents required: A. 1% osmic acid; B.\% potassium dichromate; C. 0.5% hema- 

 toxylin in 70% ale. 

 method: [small blocks of brain tissue] — > A, overnight — ♦ B, in dark, 3 or 4 changes in 

 24 hrs. — > 50% ale, 24 hrs. in dark -^ C, till stained throughout —> [celloidin or 

 paraffin sections] 

 recommended for: myelin sheaths. 



21.212 Seidelin 1911 see DS 11.121 Weigert 1904 (note) 



21.212 Sihler lest. 1907 Bohm and Oppel cit. Gad Bohm and Oppel 1907, 268 



REAGENTS REQUIRED: A. Water 75, glycerol 12, acetic acid 12, chloral hydrate 0.75; 



B. water 75, DS 11.123 Ehrlich 1886 12, glycerol 12, chloral hydrate 0.75; C. 0.1% 

 acetic acid in glycerol 



method: [pieces of fresh muscle] —> A, 18 hrs. -^ B, 3 to 10 days—* C, till differenti- 

 ated — » glycerol 



21.212 Smith and Quiegley 1937 608b, 13:491 



RE.'VGENTS REQUIRED: A. 4% ferric alum; B. water 97, acetic acid 3, hemato.xylin 1; 



C. 0.5% lithium carbonate 



method: [sections] — > water — > A, 15 mins. -^ 70% ale, short wash — » B, 30-60 mins., 

 55°C. — » rinse — * C, till blue — > wash — > balsam, via usual reagents 



