408 METHODS AND FORMULAS DS 21.212 



21.212 Spielmeyer 1930 test. 1938 Mallory Mallory 1938, 237 



REAGEi^Ts required: A. 2.5% ferric alum; B. water 100, 10% ripened hematoxylin in 



abs. ale. 5 

 method: [20-30 m frozen sections of formaldehyde-fixed material] ^ ^, 6 hrs. -^ rinse 



-^70% ale. 10 mins. — > rinse -^ A, till differentiated — > balsam, via usual reagents 

 RECOMMENDED FORt myelin sheaths. 



21.212 Tschernyschew and Karusin 1896 see DS 21.212 Pal 1887 (note) 



21.212 Vassale 1889 see DS 21.212 Weigert 1885 (note) 



21.212 "Weigert-Pal" see almost any method in this section. Gatenby and Cowdry 1937, 

 526, state Wolters 1890 to be "the standard 'Weigert-Pal' technique." 



21.212 Weigert 1885 8645, 3:230 



REAGENTS REQUIRED: A. 5% potassium dichromate; B. 3.5% copper acetate; C. water 



90, 95% ale. 10, hematoxylin 1, sat. aq. sol. lithium carbonate 1; D. ADS 21.1 Weigert 



1885 

 method: [pieces of brain] — > A, till white matter dark brown (some wks.) -^ [embed in 



celloidin] -^ B, 55°C., 2 days — > 80% ale, till required — ^ section 15-20 m — » water — > 



C, 2 hrs. to 1 day — > wash — * D, till differentiated -^ wash -^ balsam, via usual 



reagents 

 RECOMMENDED FOR: myelin sheaths. 

 note: Vassale 1889 (19460, 15 : 102) uses B after C but it is otherwise identical. Weigert 



1886 (7936a, 6:10) substitutes for A his F 6700.0000 fixative. 



21.212 Weigert 1904 see DS 11.121 Weigert 1904 



21.212 Weil 1928a method for thin sections — auct. 1879,20:392 



REAGENTS REQUIRED: A. water 100, ferric alum 2, hematoxylin 0.5; B. 4% ferric alum; 



C. ADS 21.1 Weil 1928a; D. 0.1% ammonia 

 PREPARATION OF A: Add 50 1% hematoxylin to 50 4% ferric alum. 

 method: [sections less than 30 /x thick] —^70% ale. — > water -* A, 20-30 mins., 55°C. — > 



B, 5 mins. -^ C, till differentiated -^ wash -^ D, till blue -^ balsam, via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



21.212 Weill928b method for thick sections—auci. 1879,20:32 



reagents required: A. 3% potassium dichromate; B. (see Weil 1928a, sol. A, above); 



C. 4% ferric alum; D. ADS 21.1 Weil 1928a; E. ADS 21.1 Weil 1928b A & B sols.; 

 F. 0.1% ammonia 



method: [sections more than 30 /x thick] -^ 70% ale. — > water -^ A, overnight—* wash 

 -^ B, 20-30 mins., 55°C. -^ C, 10 mins. -^ D, till differentiated -> E (A), 10 mins. 

 -^ E (B), till bleached — > [repeat E (A) — > E (B) till background sufficiently bleached] 

 -^ wash -^ F, till blue —* balsam, via usual reagents 



21.212 Wharton 1937 763, 67 :467 



reagents required: A. water 76, acetic acid 12, glycerol 12, chloral hydrate 0.75; 



B. water 76, DS 11.123 Ehrlich 12, glycerol 12, chloral hydrate 

 method: [thin organs stranded on paper] -^ A, 18 hrs. -^ B, 24 hrs. — > either glycerol 



or -^ balsam, via carbol-xylene 

 RECOMMENDED FOR: demonstration of nerves in wholemounts. 



21.212 Wolters 1890 23632, 7 :466 



REAGENTS REQUIRED: A. DS 11.113 Wolters 1890; B. F 7000.0000 Miiller 1859; C. ADS 



21.1 Pal 1887 (A & B sols.) 

 method: [15-20 m celloidin sections of Erlitzky 1877 F 4700.0000 hardened material] -^ 



A, 24 hrs., 45°C. — > B, rinse -+ rinse — > C, till differentiated -* water, wash -^ balsam, 



via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



21.212 Wolters test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 172 



REAGENTS REQUIRED: A. water 100, vanadium chloride 2, ammonium acetate 6; B. water 

 100, acetic acid 3, hematoxylin 2; C. 0.1 hydrochloric acid in 80% alcohol 



